鸡传染性支气管炎病毒感染HD11细胞的转录组分析

doi:10.11843/j.issn.0366-6964.2023.11.039

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (11): 4860-4865.doi: 10.11843/j.issn.0366-6964.2023.11.039

鸡传染性支气管炎病毒感染HD11细胞的转录组分析

陈璐, 常新宇, 沈嘉忱, 沈瑞廷, 赵振华, 侯晓林*

北京农学院动物科学技术学院, 北京 102206

收稿日期:2023-02-09 出版日期:2023-11-23 发布日期:2023-11-26
通讯作者: 侯晓林,主要从事家禽病毒学研究,E-mail:hxlsx@163.com
作者简介:陈璐(1998-),女,河北唐山人,硕士生,主要从事家禽病毒学研究,E-mail:chenluyoo@163.com
基金资助:
国家自然科学基金(31372485)

Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus

CHEN Lu, CHANG Xinyu, SHEN Jiachen, SHEN Ruiting, ZHAO Zhenhua, HOU Xiaolin*

College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China

Received:2023-02-09 Online:2023-11-23 Published:2023-11-26

摘要/Abstract

摘要： 旨在探究感染早期宿主细胞的天然免疫应答和抗病毒反应,本研究将鸡传染性支气管炎病毒(infectious bronchitis virus, IBV) Beaudette株感染鸡巨噬细胞HD11,2 h后提取细胞总RNA反转录成cDNA,采用Illumina进行转录组高通量测序。结果显示,感染组筛选出1 363个上调基因,653个下调基因。通过对差异表达基因GO和KEGG分析发现,IBV感染HD11细胞早期,差异表达基因大多与免疫反应、抗病毒作用有关,富集到的信号通路主要集中在细胞因子和细胞因子受体之间的相互作用、病毒蛋白与细胞因子或细胞因子受体之间的相互作用、TNF信号通路、MAPK信号通路等。随机挑选10个与免疫反应、抗病毒相关基因进行RT-qPCR验证,结果与转录组测序分析一致。本研究为进一步探究IBV致病机制和感染早期宿主细胞免疫反应奠定了基础。

关键词: 鸡传染性支气管炎病毒, 鸡巨噬细胞HD11, 高通量测序, 转录组

Abstract: In order to investigate the innate immune response and antiviral response of host cells at the early stage of infection, infectious bronchitis virus (IBV) Beaudette strains was used to infect chicken macrophage HD11 cells. After 2 h, total RNA was extracted from the cells into cDNA, and high throughput transcriptomic sequencing was performed by Illumina. The results showed that 1 363 up-regulated genes and 653 down-regulated genes were screened out in the infection group. According to GO and KEGG analysis, it was found that in the early stage of the infection of IBV-infected HD11 cells, the differentially expressed genes were mostly related to immune response and antiviral effect. The enriched signaling pathways mainly focus on the cytokine-cytokine receptor interaction, viral protein interaction with cytokine and cytokine receptor, TNF signaling pathway, MAPK signaling pathway, etc. Ten randomly selected genes related to immune response and antiviral were verified by RT-qPCR, and the results were consistent with the transcriptome sequencing analysis. This study lays a foundation for further investigation of the pathogenesis of IBV and host cell immune response in the early stage of infection.

Key words: infectious bronchitis virus, chicken macrophage HD11, high-throughput sequencing, transcriptome

中图分类号:

陈璐, 常新宇, 沈嘉忱, 沈瑞廷, 赵振华, 侯晓林. 鸡传染性支气管炎病毒感染HD11细胞的转录组分析[J]. 畜牧兽医学报, 2023, 54(11): 4860-4865.

CHEN Lu, CHANG Xinyu, SHEN Jiachen, SHEN Ruiting, ZHAO Zhenhua, HOU Xiaolin. Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4860-4865.

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鸡传染性支气管炎病毒感染HD11细胞的转录组分析

Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus

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