黄芪多糖对HD11鸡巨噬细胞转录组和代谢组的影响

doi:10.11843/j.issn.0366-6964.2024.03.040

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (3): 1290-1301.doi: 10.11843/j.issn.0366-6964.2024.03.040

黄芪多糖对HD11鸡巨噬细胞转录组和代谢组的影响

陈富斌^1,2, 徐国伟¹, 王磊¹, 刘琴¹, 冯海鹏¹, 张康¹, 郭志廷¹, 韩松伟¹, 刘佳惠¹, 古雪艳¹, 张景艳^1*, 李建喜^1*, Huub F. J. Savelkoul³

1. 中国农业科学院兰州畜牧与兽药研究所, 兰州 730050;
2. 甘肃农业大学动物医学院, 兰州 730070;
3. 荷兰瓦赫宁根大学细胞生物与免疫学教研组, 荷兰瓦赫宁根市

收稿日期:2023-06-29 出版日期:2024-03-23 发布日期:2024-03-27
通讯作者: 张景艳,主要从事生物转化型中兽药研发及天然免疫学研究,E-mail:zwzh1223@126.com;李建喜,主要从事中兽医与临床兽医学研究,E-mail:lzjianxil@163.com
作者简介:陈富斌(1996-),男,甘肃渭源人,硕士生,主要从事中兽医学研究,E-mail:15609312263@163.com
基金资助:
甘肃省国际合作项目（22YF7WA032）；鲁甘协作项目（22CX8NA013）；中国农业科学院创新团队项目（25-LZIHPS-06）

Effects of Astragalus Polysaccharides on Transcriptome and Metabolome of HD11 Chicken Macrophages

CHEN Fubin^1,2, XU Guowei¹, WANG Lei¹, LIU Qin¹, FENG Haipeng¹, ZHANG Kang¹, GUO Zhiting¹, HAN Songwei¹, LIU Jiahui¹, GU Xueyan¹, ZHANG Jingyan^1*, LI Jianxi^1*, HUUB F. J. Savelkoul³

1. Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agicultural Sciences, Lanzhou 730050, China;
2. College of Veterinary Medicine, Gansu Argicultural University, Lanzhou 730070, China;
3. Cell Biology and Immunology Research Group, Wageningen University, the Netherlands, Wageningen, Netherlands

Received:2023-06-29 Online:2024-03-23 Published:2024-03-27

摘要/Abstract

摘要： 旨在通过分析黄芪多糖对鸡巨噬细胞HD11转录组和代谢组的作用，探讨黄芪多糖（Astragalus polysaccharide， APS）对鸡巨噬细胞HD11的免疫调节机制。设置空白对照组（CONT组）和APS处理组（A50组），其中，A50组用含50 μg·mL^-1 APS的完全培养液培养HD11细胞12 h；CONT组用完全培养液培养HD11细胞12 h。采用代谢组学与转录组学技术分析CONT组与A50处理组细胞中差异基因和差异代谢物，并进行2个组学的联合分析。结果显示：在A50处理组与CONT组的转录组分析比较中，共检测到差异基因845个，其中上调基因415个，下调基因430个；差异表达基因主要富集在细胞因子-细胞因子受体相互作用、Toll样受体信号通路、吞噬体、AGE-RAGE等信号通路，其中，细胞因子-细胞因子受体相互作用、Toll样受体信号2条通路最为显著性；在A50处理组与CONT组的代谢组分析比较中，共筛选出差异代谢物89个，其中，下调差异代谢物为70个，上调的差异代谢物为19个。在A50处理组与CONT组的转录与代谢组联合分析中共同富集到可信度排名前十的通路有嘌呤代谢、碳代谢、磷酸戊糖途径、谷胱甘肽代谢、组氨酸代谢、氨基糖和核苷酸糖代谢、α同亚麻酸代谢、ABC转运体、铁死亡和酪氨酸代谢。APS可能是通过TLR2受体信号通路、糖酵解和磷酸戊糖途径的代谢重编程，进而发挥对HD11细胞的免疫调节作用。

关键词: 黄芪多糖, 巨噬细胞, 转录, 代谢

Abstract: This study aimed to explore the immunomodulatory mechanism of Astragalus polysaccharide (APS) on HD11 in chicken macrophages by analyzing the effects of APS on the transcriptomes and metabolomes of HD11. A blank control group (CONT group) and an APS treatment group (A50 group) were set up, in which HD11 cells of the A50 group was treated with complete culture medium contain 50 μg·mL^-1 APS for 12 h; and HD11 cells of the CONT group were cultured in complete culture medium for 12 h. Metabolomics and transcriptomic techniques were used to analyze the differential genes and differential metabolites in CONT and A50 treated cells, and the combined analysis of the two omics was performed. Results:A total of 845 differential genes were detected in the transcriptome analysis between the A50 treatment group and the CONT group, including 415 up-regulated genes and 430 down-regulated genes. Differentially expressed genes were mainly concentrated in cytokine-cytokine receptor interaction, toll-like receptor signaling pathway, phagosome and AGE-RAGE signaling pathways, among which cytokine-cytokine receptor interaction and toll-like receptor signaling pathways were the most significant. In the metabolome analysis and comparison between the A50 treatment group and the CONT group, a total of 89 differential metabolites were screened, of which 70 were down-regulated and 19 were up-regulated. In the combined analysis of transcription and metabolism of the A50 treatment group and the CONT group, the top ten trusted pathways were purine metabolism, carbon metabolism, pentose phosphate pathway, glutathione metabolism, histidine metabolism, amino sugar and nucleotide sugar metabolism, α-homolenic acid metabolism, ABC transporter, iron death and tyrosine metabolism. APS may exert immunomodulatory effects on HD11 cells through the metabolic reprogramming of toll-like receptor signaling pathway, glycolysis and pentose phosphate pathway.

Key words: astragalus polysaccharide, macrophage, transcription, metabolism

中图分类号:

S853.74

陈富斌, 徐国伟, 王磊, 刘琴, 冯海鹏, 张康, 郭志廷, 韩松伟, 刘佳惠, 古雪艳, 张景艳, 李建喜, Huub F. J. Savelkoul. 黄芪多糖对HD11鸡巨噬细胞转录组和代谢组的影响[J]. 畜牧兽医学报, 2024, 55(3): 1290-1301.

CHEN Fubin, XU Guowei, WANG Lei, LIU Qin, FENG Haipeng, ZHANG Kang, GUO Zhiting, HAN Songwei, LIU Jiahui, GU Xueyan, ZHANG Jingyan, LI Jianxi, HUUB F. J. Savelkoul. Effects of Astragalus Polysaccharides on Transcriptome and Metabolome of HD11 Chicken Macrophages[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1290-1301.

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黄芪多糖对HD11鸡巨噬细胞转录组和代谢组的影响

Effects of Astragalus Polysaccharides on Transcriptome and Metabolome of HD11 Chicken Macrophages

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