赤芍黄柏制剂对致病性大肠杆菌人工感染雏鸡的肠道菌群多样性的影响

doi:10.11843/j.issn.0366-6964.2021.08.019

摘要/Abstract

摘要： 为了解不同浓度赤芍黄柏制剂对致病性大肠杆菌（Escherichia coli）感染的雏鸡肠道菌群多样性的影响，本研究对240只15日龄健康雏鸡随机编号后分为空白对照组（CON）、感染组（BC）、低剂量治疗组（TL）和高剂量治疗组（TH）4个组，除对照组外，其余各组（BC、TL、TH）均使用致病性大肠杆菌进行感染，感染0.5 d后，分别用不同浓度的赤芍黄柏制剂对TL组（4 mL·L^-1灭菌水）和TH组（12 mL·L^-1灭菌水）进行饮水治疗，治疗5 d后统计各组发病率及死亡率，采用高通量测序技术检测雏鸡十二指肠肠道菌群多样性。结果显示，与感染组相比，TH组显著降低了雏鸡的发病率和死亡率（P<0.05）。菌群分析显示，赤芍黄柏制剂处理改变了雏鸡肠道菌群多样性组成，提高了乳杆菌属的丰度。CON组优势菌群以厚壁菌门、肠球菌属为主；BC组肠道优势菌群主要为厚壁菌门、变形菌门、肠球菌属、大肠杆菌属及葡萄球菌属；TL组优势菌群以厚壁菌门、乳杆菌属、肠球菌属及葡萄球菌属为主；TH组优势菌群以厚壁菌门、乳杆菌属为主。热图分析显示，乳杆菌属与发病率和死亡率呈负相关，大肠杆菌属与发病率和死亡率呈正相关（P<0.05）。综上，大肠杆菌感染及赤芍黄柏制剂治疗改变了雏鸡肠道菌群多样性及组成，而这种肠道菌群的变化可能对雏鸡腹泻发病及抗病力产生重要影响。

关键词: 赤芍黄柏制剂, 雏鸡, 致病性大肠杆菌, 肠道菌群, 高通量测序

Abstract: The study aimed to understand the effects of Radix paeoniae rubra and Cortex phellodendri preparation on the intestinal flora diversity of chickens infected with pathogenic Escherichia coli. Two hundred and forty 15-day-old chicks were randomly divided into 4 groups:blank control group (CON), infection group (BC), low-dose treatment group (TL) and high-dose treatment group (TH). Except for the control group, all the treatment groups (BC, TL, TH) were infected with pathogenic Escherichia coli. TL (4 mL·L^-1 sterilized water) and TH (12 mL·L^-1 sterilized water) groups were treated with different concentration of Radix paeoniae rubra and Cortex phellodendri preparation by drinking water after infection of 0.5 d. After 5 days of treatment, the morbidity and mortality of each group were calculated, and high-throughput sequencing technology was used to detect the diversity of bacterial community in the duodenum of chicks. The results showed that, compared with the BC group, the TH group significantly reduced the morbidity and mortality of chicks (P<0.05). Bacterial community analysis showed that, the preparation processing changed the intestinal bacterial community in the chicks. The dominant bacteria were Firmicutes and Enterococcus in CON group; Firmicutes, Proteobacteria, Enterococcus, Escherichia and Staphylococcus in BC group; Firmicutes, Lactobacillus, Enterococcus and Staphylococcus in TL group; Firmicutes and Lactobacillusin in TH group. The heatmap analysis showed that Lactobacillus was negatively correlated with morbidity and mortality, while Escherichia was positively correlated with morbidity and mortality (P<0.05). In summary, the infection of Escherichia coli and the preparation treatment of Radix paeoniae rubra and Cortex phellodendri changed the diversity and composition of intestinal bacterial community in chicks, and the changes of intestinal bacterial community may have an important impact on the incidence and disease resistance of diarrhea in chicks.

Key words: Radix paeoniae rubra and Cortex phellodendri preparation, chick, pathogenic Escherichia coli, intestinal bacterial community, high throughput sequencing

中图分类号:

郭双, 蔡小利, 胡宗福, 牛化欣, 马德慧, 王梓, 付丽云, 陈志强, 刘锴. 赤芍黄柏制剂对致病性大肠杆菌人工感染雏鸡的肠道菌群多样性的影响[J]. 畜牧兽医学报, 2021, 52(8): 2265-2274.

GUO Shuang, CAI Xiaoli, HU Zongfu, NIU Huaxin, MA Dehui, WANG Zi, FU Liyun, CHEN Zhiqiang, LIU Kai. Effect of Radix Paeoniae Rubra and Cortex Phellodendri Preparation on Intestinal Bacterial Community Diversity of Chickens Artificially Infected with Pathogenic Escherichia coli[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2265-2274.

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