藏鸡KLF15基因克隆、组织表达谱及其表达与肌内脂肪含量相关性的研究

doi:10.11843/j.issn.0366-6964.2019.02.004

Abstract

Abstract:

The objective of this study was to obtain the sequence of KLF15 gene of Tibetan chicken,clarify its tissue and temporal expression profiles and to analyze the relationship between its expression and the intramuscular fat (IMF) content. Ten healthy Tibetan chicken(5 cock and 5 hen) on 1, 81, 119, 154 and 210 days old were selected, respectively as experimental animals. RT-PCR technology was used to clone KLF15 gene of Tibetan chicken, and bioinformatics softwares were used to analyze the biological characteristics of KLF15. The expression profiles of KLF15 gene in different tissues and different developmental stages was detected by fluorescence quantitative PCR, and its correlation with IMF content was analyzed. The result showed that the cloned Tibetan chicken KLF15 gene sequence was 1 288 bp in length (GenBank accession number:KY747450), an open reading frame of 1 212 bp, encoding 403 amino acids, and it was a hydrophilic unstable basic protein with 3 zinc finger structures. The homology between the nucleotide and amino acid sequences of KLF15 gene of Tibetan chicken and Gallus gallus were 99%. KLF15 mRNA was widely expressed in all detected tissues of Tibetan chicken, and the expression level was the highest in lung, which is significantly higher than that in other tissues (P<0.01). The expression level of KLF15 gene in breast muscle of Tibetan chicken of 1 day old was higher than that of the other age groups, and declined with the increasing age. The expression level of KLF15 gene in leg muscle of Tibetan chicken of 119 days old was higher than that of the other age groups, and the change of the overall expression level showed a trend of decreasing first, then rising, finally falling. The expression level of KLF15 gene before 154 days old was positively correlated with IMF content in Tibetan cocks,after 154 days old, the correlation was reverse, and the Tibetan hens mainly showed a weak negative correlation in all age groups. These results provide the basic data for further research on the role of KLF15 in IMF deposition of Tibetan chicken.

CLC Number:

S831.2

WANG Yingming, XU Ya'ou, WANG Zhimin, XU Longyang, YANG Lei, LIN Yaqiu. Studies on the Cloning of KLF15 Gene, Tissue Expression Profile and the Association between Its Expression and Intramuscular Fat Content in Tibetan Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 261-270.

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Studies on the Cloning of KLF15 Gene, Tissue Expression Profile and the Association between Its Expression and Intramuscular Fat Content in Tibetan Chicken

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