副猪嗜血杆菌SC096株荚膜多糖抗猪肺泡巨噬细胞吞噬能力的研究

doi:10.11843/j.issn.0366-6964.2016.06.019

Abstract

Abstract:

The aim of the experiment was to clarify the role of the capsular polysaccharide in anti-porcine alveolar macrophage phagocytosis of Haemophilus parasuis.The anti-phagocytosis test was conducted by using indirect immunofluorescence method and flow cytometry with H.parasuis SC096 wild-type strain，and capsular deficient strain.The results showed that the H.parasuis SC096 wild-type strains were observed mainly outside the macrophages，while capsular polysaccharide-deficient were observed in macrophages by indirect immunofluorescence experiments.Flow cytometry results showed that the total macrophage phagocytosis rate was 12.51% and swallowed efficiency was 89.61% in the wild-type strain group；the total macrophage phagocytosis rate was 27.18% and swallowed efficiency was 91.06% in the capsular polysaccharide-deficient group.By adding exogenous capsular polysaccharide，total macrophage phagocytosis rate was reduced to 2.72%，swallowed efficiency was reduced to 32.72% in the wild-type strain group；the total macrophage phagocytosis rate dropped to 3.59%，swallowed efficiency was nearly unchanged at 91.36% in the capsular polysaccharide-deficient group.The ability of anti-macrophage phagocytosis of H.parasuis was reduced through removing capsular polysaccharide，which demonstrated that capsular polysaccharide may inhibit the ability of macrophage phagocytosis.

CLC Number:

S852.61

ZHOU Qi，HE Xian-hui，YANG Qiao-mei，FENG Sai-xiang，FAN Hui-ying，LIAO Ming. Role of Capsular Polysaccharide in Haemophilus parasuis SC096 Strain Anti-Porcine Alveolar Macrophage Phagocytosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1232-1238.

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Role of Capsular Polysaccharide in Haemophilus parasuis SC096 Strain Anti-Porcine Alveolar Macrophage Phagocytosis

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