定量蛋白质组学技术分析猪繁殖与呼吸综合征病毒感染肺泡巨噬细胞的差异表达蛋白质

doi:10.11843/j.issn.0366-6964.2016.06.018

Abstract

Abstract:

The purpose of our research was to study the differential expression proteins of Porcine reproductive and respiratory syndrome virus (PRRSV) infected porcine alveolar macrophage (PAMs) relative to the un-infected control PAMs.Differential proteins in response to PRRSV infection were analyzed by High Performance Liquid Chromatography coupled with tandem mass spectrometry，and then identified using software Max-Quant 1.0.7.4 combining with the protein database PaxDb.Thirty-nine differential proteins were identified in the PRRSV infected group，including thirty up-regulated proteins and nine down-regulated proteins.The up-regulation of SPP1，IFIT3 and IL-8，and down-regulation of STAT3 in PRRSV infected PAMs were verified by Western blot and ELISA.This study provides new evidences for further studies on the molecular mechanism of PRRSV pathogenesis by using LC-MS/MS.

CLC Number:

S858.285.3

LIU Long，CHEN Hong-ying. Analysis of Differential Expression Proteins in Porcine Reproductive and Respiratory Syndrome Virus Infected Pulmonary Alveolar Macrophage by Quantitative Proteomics[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1222-1231.

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Analysis of Differential Expression Proteins in Porcine Reproductive and Respiratory Syndrome Virus Infected Pulmonary Alveolar Macrophage by Quantitative Proteomics

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