牛蒡苷元对猪圆环病毒2型感染仔猪体内病毒复制及组织病理影响

doi:10.11843/j.issn.0366-6964.2018.12.016

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (12): 2672-2679.doi: 10.11843/j.issn.0366-6964.2018.12.016

牛蒡苷元对猪圆环病毒2型感染仔猪体内病毒复制及组织病理影响

陈洁^1,2, 吴利军², 陈夏冰², 刘晓丽³, 金尔光², 邵志勇², 杨文海², 何斌², 童伟文², 刘武^2*, 杨汉春^1*

1. 中国农业大学动物医学院, 北京 100193;
2. 武汉市农业科学院畜牧兽医研究所, 武汉 430208;
3. 华中农业大学动物医学国家级实验教学示范中心, 武汉 430070

收稿日期:2018-07-09 出版日期:2018-12-23 发布日期:2018-12-23
通讯作者: 杨汉春,教授,博导,E-mail:yanghanchun1@cau.edu.cn;刘武,正高职畜牧师,E-mail:1974813043@qq.com
作者简介:陈洁(1981-),女,湖北武汉人,高级兽医师,博士,主要从事畜禽传染病防控技术研究,E-mail:chen_jie_999@163.com
基金资助:
动物胚胎工程及分子育种湖北省重点实验室开放项目（KLAEMB-2016-05）；武汉市畜牧兽医科学研究所院士专家工作站（武科协[2015]31号）

Effects of Arctigenin on Viral Replication and Histopathology after Porcine Circovirus Type 2 Infection of Piglets

CHEN Jie^1,2, WU Li-jun², CHEN Xia-bing², LIU Xiao-li³, JIN Er-guang², SHAO Zhi-yong², YANG Wen-hai², HE Bin², TONG Wei-wen², LIU Wu^2*, YANG Han-chun^1*

1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
2. Animal Husbandry and Veterinary Institute of Wuhan Academy of Agricultural Sciences, Wuhan 430208, China;
3. National Demonstration Center for Experimental Animal Medical Education, Huazhong Agricultural University, Wuhan 430070, China

Received:2018-07-09 Online:2018-12-23 Published:2018-12-23

摘要/Abstract

摘要：

为了评价中药活性成分牛蒡苷元（arctigenin，ACT）对猪圆环病毒2型（porcine circovirus type 2，PCV2）人工感染仔猪体内病毒的复制及组织病变的影响，选取16头30日龄健康断奶仔猪，随机分为空白对照组、攻毒对照组、给药组1（20 μg·kg^-1）和给药组2（50 μg·kg^-1）。两个给药组连续肌内注射给药3 d（空白和攻毒对照组注射生理盐水）后进行攻毒，在攻毒前2 d和攻毒后0、7、14、21 d分别采集仔猪外周血进行PCV2定量检测；攻毒后21 d将试验仔猪全部剖杀，采集腹股沟淋巴结、肠系膜淋巴结、扁桃体、肺和脾组织进行PCV2定量检测和组织病理学观察。结果显示，两个给药组均能显著降低攻毒后7、14和21 d仔猪外周血病毒核酸拷贝数（P<0.05或P<0.01）；攻毒后21 d，给药组1（20 μg·kg^-1）能显著抑制腹股沟淋巴结（P<0.05）、肠系膜淋巴结（P<0.05）组织中PCV2的增殖，给药组2（50 μg·kg^-1）能显著抑制腹股沟淋巴结（P<0.001）、肠系膜淋巴结（P<0.01）、扁桃体（P<0.05）和肺（P<0.05）组织中PCV2的增殖；并且两个剂量均能减轻PCV2感染造成的上述器官组织的病理损伤。ACT对PCV2在人工感染仔猪体内的增殖有明显的抑制效果，并能减轻由PCV2感染引起的病理变化。

Abstract:

This experiment was conducted to study the effects of arctigenin (ACT) on viral replication and histopathology after porcine circovirus type 2 (PCV2) infection of piglets. Sixteen 30-day-old healthy piglets were randomly assigned to 4 groups. Drug treated groups were intramuscular injected of ACT on dose of 20 and 50 μg·kg^-1 respectively. Control group and virus challenge group were intramuscular injected with normal saline. After continuous injection for 3 days, the two drug treated groups and virus challenge group were inoculated with PCV2 and control group were inoculated with normal saline. The peripheral blood samples were collected on day -2, 0, 7, 12, 21 and the viral load in those serums were detected by qPCR. The piglets were sacrificed on day 21 after PCV2 infection, and the lung, spleen, tonsilla, mesenterium lymph nodes, inguinal lymph nodes were collected for PCV2 quantitative detection and histopathological observation. The results showed that both ACT treated groups could significantly inhibit the copy number of PCV2 in piglets peripheral blood after 7, 14 and 21 days of infection (P<0.05 or P<0.01). The results of viral load in tissues showed that, PCV2 proliferation in the tissues of inguinal lymph nodes (P<0.05) and mesenteric lymph nodes (P<0.05) were significantly inhibited in the drug group 1 (20 μg·kg^-1), and the viral proliferation in inguinal lymph nodes (P<0.001), mesenteric lymph nodes (P<0.01), tonsils (P<0.05) and lungs (P<0.05) were significantly inhibited in the dose group 2 (50 μg·kg^-1). Further histopathological sections and histopathological scoring results showed that two doses of ACT could reduce the pathological damage of the above-mentioned organ tissue caused by PCV2 infection. These results showed that ACT had a significant inhibitory effect on the proliferation of PCV2 in artificially infected piglets and could reduce the pathological changes caused by PCV2 infection.

中图分类号:

陈洁, 吴利军, 陈夏冰, 刘晓丽, 金尔光, 邵志勇, 杨文海, 何斌, 童伟文, 刘武, 杨汉春. 牛蒡苷元对猪圆环病毒2型感染仔猪体内病毒复制及组织病理影响[J]. 畜牧兽医学报, 2018, 49(12): 2672-2679.

CHEN Jie, WU Li-jun, CHEN Xia-bing, LIU Xiao-li, JIN Er-guang, SHAO Zhi-yong, YANG Wen-hai, HE Bin, TONG Wei-wen, LIU Wu, YANG Han-chun. Effects of Arctigenin on Viral Replication and Histopathology after Porcine Circovirus Type 2 Infection of Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2672-2679.

参考文献

[1] TISCHER I, GELDERBLOM H, VETTERMANN W, et al. A very small porcine virus with circular single-stranded DNA[J]. Nature, 1982, 295(5844):64-66.
[2] ELLIS J. Porcine circovirus:a historical perspective[J]. Veterin Pathol, 2014, 51(2):315-327.
[3] KU X, CHEN F, LI P, et al. Identification and genetic characterization of porcine circovirus type 3 in China[J]. Transbound Emerg Dis, 2017, 64(3):703-708.
[4] ZHAO D, WANG X B, GAO Q Q, et al. Retrospective survey and phylogenetic analysis of porcine circovirus type 3 in Jiangsu province, China, 2008 to 2017[J]. Arch Virol, 2018, 163(9):2531-2538.
[5] GILLESPIE J, OPRIESSNIG T, MENG X J, et al. Porcine circovirus type 2 and porcine circovirus-associated disease[J]. J Veterin Intern Med, 2009, 23(6):1151-1163.
[6] JIANG C G, WANG G, TU Y B, et al. Genetic analysis of porcine circovirus type 2 in China[J]. Arch Virol, 2017, 162(9):2715-2726.
[7] 李娇,王文秀,谢金文,等. 我国猪圆环病毒病的流行现状分析[J]. 养猪, 2016(6):97-100.
LI J, WANG W X, XIE J W,et al. Analysis of the prevalence of porcine circovirus in China[J]. Swine Production, 2016(6):97-100. (in Chinese)
[8] 施开创,杨汉春,郭鑫,等. 猪生殖与呼吸综合征病毒和猪圆环病毒2型共感染对猪外周血天然免疫细胞含量的影响[J]. 中国兽医科学, 2007, 37(1):7-11.
SHI K C, YANG H C, GUO X, et al. Effects of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 co-infection on numbers of native immune cells in peripheral blood of piglets[J]. Veterinary Science in China, 2007, 37(1):7-11. (in Chinese)
[9] ZHOU N, FAN C M, LIU S, et al. Cellular proteomic analysis of porcine circovirus type 2 and classical swine fever virus coinfection in porcine kidney-15 cells using isobaric tags for relative and absolute quantitation-coupled LC-MS/MS[J]. Electrophoresis, 2017, 38(9-10):1276-1291.
[10] 高雪敏. 中药学[M]. 北京:中国中医药出版社, 2002:76-77.
GAO X M. Traditional Chinese medicine[M]. Beijing:China Press of Traditional Chinese Medicine, 2002:76-77. (in Chinese)
[11] 马英华,张晓娟. 牛蒡子药物应用的研究进展[J]. 中医药信息, 2017, 34(2):116-119.
MA Y H, ZHANG X J.Research progress on the application of Chinese traditional medicine fructus arctii[J]. Information on Traditional Chinese Medicine, 2017, 34(2):116-119. (in Chinese)
[12] ZHANG W Z, JIANG Z K, HE B X, et al. Arctigenin protects against lipopolysaccharide-induced pulmonary oxidative stress and inflammation in a mouse model via suppression of MAPK, HO-1, and iNOS signaling[J]. Inflammation, 2015, 38(4):1406-1414.
[13] HAYASHI K, NARUTAKI K, NAGAOKA Y, et al. Therapeutic effect of arctiin and arctigenin in immunocompetent and immunocompromised mice infected with influenza A virus[J]. Biol Pharm Bull, 2010, 33(7):1199-1205.
[14] 陈夏冰,付书林,何斌,等. 牛蒡苷元对疫苗免疫猪抗体和T细胞亚群的影响[J]. 中国兽医杂志, 2015, 51(4):52-55.
CHEN X B, FU S L, HE B,et al. Effects of arctigenin on antibody and T lymphocytes subpopulations in the immune response to vaccine[J]. Chinese Journal of Veterinary Medicine, 2015, 51(4):52-55. (in Chinese)
[15] 薛芳喜,姚景春,刘奋. 牛蒡子苷元对小鼠免疫功能的影响[J]. 中华中医药学刊, 2016, 34(2):350-352.
XUE F X, YAO J C, LIU F.Effect of arctigenin on immune function in mice[J]. Chinese Archives of Traditional Chinese Medicine, 2016, 34(2):350-352. (in Chinese)
[16] VLIETINCK A J, DEBRUYNE T, APERS S, et al. Plant-derived leading compounds for chemotherapy of human immunodeficiency virus (HIV) infection[J]. Planta Med, 1998, 64(2):97-109.
[17] 符林春,徐培平,刘妮,等. 牛蒡子苷元复方抗流感病毒的实验研究[J]. 中药新药与临床药理, 2008, 19(4):266-269.
FU L C, XU P P, LIU N, et al. Antiviral effect of arctigenin compound on influenza virus[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2008, 19(4):266-269. (in Chinese)
[18] SWARUP V, GHOSH J, MISHRA M K, et al. Novel strategy for treatment of Japanese encephalitis using arctigenin, a plant lignan[J]. J Antimicrob Chemother, 2008, 61(3):679-688.
[19] CHEN J, LI W T, JIN E G, et al. The antiviral activity of arctigenin in traditional Chinese medicine on porcine circovirus type 2[J]. Res Veterin Sci, 2016, 106:159-164.
[20] DARWICH L, SEGALES J, MATEU E. Pathogenesis of postweaning multisystemic wasting syndrome caused by porcine circovirus 2:an immune riddle[J]. Arch Virol, 2004, 149:857-874.
[21] KRAKOWKA S, ELLIS J A, MCNEILLY F, et al. Immunologic features of porcine circovirus type 2 infection[J]. Viral Immunol, 2002, 15(4):567-582.
[22] CHAE C. A review of porcine 2-circovirus associated syndromes and diseases[J]. Veterin J, 2005, 169(3):326-336.

牛蒡苷元对猪圆环病毒2型感染仔猪体内病毒复制及组织病理影响

Effects of Arctigenin on Viral Replication and Histopathology after Porcine Circovirus Type 2 Infection of Piglets

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