基于1H-NMR技术的产后卵巢静止奶牛乳清和血清代谢谱分析

doi:10.11843/j.issn.0366-6964.2018.12.009

摘要/Abstract

摘要：

旨在应用代谢组学技术筛选产后卵巢静止奶牛乳清和血清的差异代谢物，明确其与奶牛卵巢静止的关系。本研究选取产后45~60 d的正常发情奶牛和卵巢静止奶牛各14头进行试验。应用一维氢谱核磁共振（¹H-NMR）技术对两组奶牛的乳清和血清进行检测，结合多元统计分析和单变量分析，筛选出组间差异代谢物，并进行生物信息学分析，阐明差异代谢物参与的代谢通路与奶牛卵巢静止的关系。结果表明，在乳清和血清中分别筛选出13种差异代谢物。与发情奶牛相比，卵巢静止奶牛乳清中6种差异代谢物含量升高：琥珀酸、磷酸肌酸、甘氨酸、肌醇、乙醇酸、乳清酸，7种下降：丙氨酸、肌酐、磷酸胆碱、乳糖、牛磺酸、半乳糖、葡萄糖-1-磷酸；血清中4种差异代谢物升高：β-羟丁酸、乙酸、谷氨酰胺、甘氨酸，9种降低：丙氨酸、琥珀酸、柠檬酸、肌酐、磷酸胆碱、葡萄糖、肌醇、酪氨酸、组氨酸。两组奶牛乳清差异代谢物主要参与牛磺酸和亚牛磺酸代谢、半乳糖代谢、原代胆汁酸生物合成等。血清差异代谢物主要参与丙氨酸、天冬氨酸和谷氨酸代谢、乙醛酸和二羧酸代谢、柠檬酸循环、苯丙氨酸、酪氨酸和色氨酸生物合成等。本研究获得了卵巢静止奶牛乳清和血清的代谢谱及其差异代谢物，结果表明了主要的糖代谢、脂代谢和氨基酸代谢的异常途径在奶牛产后卵巢静止发生中所起的作用，为今后深入研究奶牛产后卵巢静止发生机制和防治策略提供了新的方向。

Abstract:

The study aimed to use metabolomics techniques to screen the differential metabolites in whey and serum of ovarian quiescence cows and to determine the relationship between differential metabolites and the ovary quiescence of cows. In the study, 14 estrus cows and 14 ovarian quiescence cows from 45-60 d postpartum were selected for testing. The whey and serum of cows in the two groups were tested using ¹H-NMR technique. Differential metabolites in whey and serum of cows between the two groups were screened by multivariate statistical analysis and univariate analysis. Bioinformatics analysis was performed to elucidate the relationship between metabolic pathways involved in differential metabolites and ovarian quiescence of dairy cows. The results showed that 13 differential metabolites in cows between the two groups were screened in whey and serum, respectively. Compared with estrus cows, the contents of 6 differential metabolites increased in the whey of ovarian quiescence cows, which were succinate, creatine phosphate, glycine, myo-inositol, glycolate,orotate; and the contents of 7 differential metabolites decreased, which were alanine, creatinine, O-phosphorylcholine, lactose, taurine, galactose and glucose-1-phosphate. The contents of 4 differential metabolites increased in the serum of ovarian quiescence cows, which were β-hydroxybutyrate, acetate, glutamine, glycine; and the contents of 9 differential metabolites reduced, which were alanine, succinate, citrate, creatinine, O-phosphocholine, glucose, myo-inositol, tyrosine and histidine. The differential metabolites in whey of cows between the two groups were mainly involved in the metabolism of taurine and hypotaurine, the metabolism of galactose, and the biosynthesis of primary bile acids. The differential metabolites in serum were mainly involved in alanine, aspartate and glutamate metabolism, glyoxylic acid and dicarboxylic acid metabolism, citric acid cycle, phenylalanine, tyrosine and tryptophan biosynthesis.In this study, the metabolic profiles and differential metabolites in whey and serum of ovarian quiescence cows were obtained. The results showed that the main abnormal pathways of glucose, lipid and amino acid metabolism played a role in postpartum ovarian quiescence in dairy cows, which provided a new direction for further studying the mechanism and prevention strategy of postpartum ovarian quiescence in dairy cows.

中图分类号:

S823.3

范子玲, 宋玉锡, 张江, 赵畅, 白云龙, 孙书函, 夏成. 基于¹H-NMR技术的产后卵巢静止奶牛乳清和血清代谢谱分析[J]. 畜牧兽医学报, 2018, 49(12): 2602-2611.

FAN Zi-ling, SONG Yu-xi, ZHANG Jiang, ZHAO Chang, BAI Yun-long, SUN Shu-han, XIA Cheng. Analysis of Metabolic Profiles of Whey and Serum in Postpartum Dairy Cows with Ovary Quiescence Based on ¹H-NMR[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2602-2611.

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基于¹H-NMR技术的产后卵巢静止奶牛乳清和血清代谢谱分析

Analysis of Metabolic Profiles of Whey and Serum in Postpartum Dairy Cows with Ovary Quiescence Based on ¹H-NMR

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