荷斯坦牛血浆抗缪勒氏管激素浓度的影响因素分析及遗传参数估计

doi:10.11843/j.issn.0366-6964.2022.01.016

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (1): 161-168.doi: 10.11843/j.issn.0366-6964.2022.01.016

荷斯坦牛血浆抗缪勒氏管激素浓度的影响因素分析及遗传参数估计

万涛¹, 王澳¹, 张海亮¹, 胡丽蓉¹, 赵善江², 张翰霖³, 王炎³, 郭刚³, 俞英¹, 王雅春^1*

1. 中国农业大学动物科学技术学院, 北京 100193;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 北京首农畜牧发展有限公司, 北京 100029

收稿日期:2021-05-11 出版日期:2022-01-23 发布日期:2022-01-26
通讯作者: 王雅春,主要从事分子及数量遗传学研究,E-mail:wangyachun@cau.edu.cn
作者简介:万涛(2000-),男,湖北武汉人,本科生,主要从事动物科学研究,E-mail:3258594702@qq.com
基金资助:
现代农业（奶牛）产业技术体系建设专项资金（CARS-36）；北京三元种业科技股份有限公司自立科研课题（SYZYZ20190005）；长江学者和创新团队发展计划（IRT_15R62）

Influencing Factors and Genetic Parameters of Plasma Anti-Müllerian Hormone Concentration in Holstein Cows

WAN Tao¹, WANG Ao¹, ZHANG Hailiang¹, HU Lirong¹, ZHAO Shanjiang², ZHANG Hanlin³, WANG Yan³, GUO Gang³, YU Ying¹, WANG Yachun^1*

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Beijing Sunlon Livestock Development Co. Ltd., Beijing 100029, China

Received:2021-05-11 Online:2022-01-23 Published:2022-01-26

摘要/Abstract

摘要： 为探究荷斯坦牛血浆抗缪勒氏管激素（anti-Müllerian hormone，AMH）浓度的群体特征和影响因素，并初步估计其遗传参数，本研究采集了北京地区某规模化牛场398头健康泌乳母牛共625份血样，采用ELISA酶免法测定血浆AMH浓度，利用SAS 9.2软件的MIXED过程建立模型，分析血浆AMH浓度的影响因素；此外，基于DMU软件使用单性状动物模型对血浆AMH浓度进行方差组分和遗传参数估计，并计算AMH与奶牛部分繁殖性状之间的近似遗传相关。结果表明，北京地区荷斯坦牛血浆AMH浓度为（96.30±14.25） ng·L^-1；采样季节对血浆AMH浓度有极显著影响（P<0.01），胎次、妊娠天数以及泌乳天数对血浆AMH浓度均无显著影响；血浆AMH浓度属于中等遗传力性状，遗传力为（0.10±0.08）；血浆AMH浓度与产犊后首次配种间隔、青年牛和经产牛的首末次配种间隔及死产率均存在中高遗传相关，近似遗传相关系数介于0.3~0.7之间。综上，本研究对AMH浓度的影响因素和遗传参数进行了初步分析，为进一步研究荷斯坦牛AMH浓度的遗传基础提供了参考，有助于通过AMH浓度对奶牛的繁殖性能进行研究。

关键词: 抗缪勒氏管激素, 荷斯坦牛, 影响因素, 遗传分析

Abstract: The objective of this study was to explore the population characteristics and influencing factors of plasma anti-Müllerian hormone (AMH) concentration in Holstein cows, and to estimate its genetic parameters. A total of 625 blood samples were collected from 398 healthy Hols-tein cattle in a large-scale dairy farm in Beijing. The plasma AMH concentration was determined by ELISA method. The MIXED procedure in SAS 9.2 was used to analyze the influencing factors of the plasma AMH concentration. A single trait animal model was used to estimate the variance components and genetic parameters of the plasma AMH concentration and the approximately genetic correlations between the plasma AMH concentration and some reproductive traits were calculated by DMU. The results from this study showed that the mean of the plasma AMH concentration in Holstein cattle was (96.30±14.25) ng·L^-1. The plasma AMH concentration was significantly (P<0.01) influenced by the sampling season, and was not significantly influenced by the parity, pregnant stage and lactation stage. The plasma AMH concentration was a moderately heritable trait with a heritability of (0.10±0.08). The plasma AMH concentration was associated with the interval from calving to the first service, interval from the first to the last inseminations and stillbirth in heifer and cow with moderate to high genetic correlations and approximate genetic correlation coefficients ranging from 0.3 to 0.7. In conclusion, this study provided a reference for further research on the genetic basis of the plasma AMH concentration in Holstein cattle, which was helpful to investigate the reproductive performance of dairy cows by AMH concentration.

Key words: anti-Müllerian hormone, Holstein cattle, influencing factors, genetic analysis

中图分类号:

S823.3

万涛, 王澳, 张海亮, 胡丽蓉, 赵善江, 张翰霖, 王炎, 郭刚, 俞英, 王雅春. 荷斯坦牛血浆抗缪勒氏管激素浓度的影响因素分析及遗传参数估计[J]. 畜牧兽医学报, 2022, 53(1): 161-168.

WAN Tao, WANG Ao, ZHANG Hailiang, HU Lirong, ZHAO Shanjiang, ZHANG Hanlin, WANG Yan, GUO Gang, YU Ying, WANG Yachun. Influencing Factors and Genetic Parameters of Plasma Anti-Müllerian Hormone Concentration in Holstein Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 161-168.

参考文献 24

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荷斯坦牛血浆抗缪勒氏管激素浓度的影响因素分析及遗传参数估计

Influencing Factors and Genetic Parameters of Plasma Anti-Müllerian Hormone Concentration in Holstein Cows

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