母牛不同发情阶段唾液化合物的比较分析

doi:10.11843/j.issn.0366-6964.2023.11.019

畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (11): 4636-4652.doi: 10.11843/j.issn.0366-6964.2023.11.019

母牛不同发情阶段唾液化合物的比较分析

董智豪^1,2, 时玉新³, 郭冠华^2,4, 原开敏^2,5, 修豪宇^2,4, 汪超^2,5, 白俊艳^1*, 王栋^2*

1. 河南科技大学动物科技学院, 洛阳 471203;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 内蒙古赤峰市敖汉旗农牧局, 赤峰 024300;
4. 山西农业大学动物科学学院, 太谷 030801;
5. 吉林农业大学动物科学技术学院, 长春 130118

收稿日期:2023-06-15 出版日期:2023-11-23 发布日期:2023-11-26
通讯作者: 白俊艳,主要从事动物遗传育种与繁殖研究,E-mail:junyanbai@163.com;王栋,主要从事动物遗传育种与繁殖研究,E-mail:dwangcn2002@vip.sina.com
作者简介:董智豪(1998-),男,安徽肥西人,硕士,主要从事动物遗传育种与繁殖研究,E-mail:18538564084@163.com
基金资助:
国家自然科学基金(32072739);家畜产业技术体系北京市创新团队(BAIC05-2022)

Comparative Analysis of Salivary Compounds in Different Estrous Stages of Cows

DONG Zhihao^1,2, SHI Yuxin³, GUO Guanhua^2,4, YUAN Kaimin^2,5, XIU Haoyu^2,4, WANG Chao^2,5, BAI Junyan^1*, WANG Dong^2*

1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471203, China;
2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Aohan Animal Husbandry Bureau, Chifeng 024300, China;
4. College of Animal Science, Shanxi Agricultural University, Taigu 030801, China;
5. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China

Received:2023-06-15 Online:2023-11-23 Published:2023-11-26

摘要/Abstract

摘要： 旨在基于公、母牛性信息素嗅觉交流的事实,为安静发情鉴定技术的研发提供重要参考。本研究预选择石家庄天泉良种奶牛有限公司的25头3~5周龄健康、产后15~35 d的可繁荷斯坦母牛,联合使用人工观察、活动量监测、直肠检查和B超检测等方法,对荷斯坦母牛进行发情鉴定,并区分正常发情和安静发情,共采集到4只正常发情和5只安静发情母牛的不同发情阶段的唾液样品,后利用固相微萃取-气相色谱-质谱联用仪(SPME-GC-MS),鉴定分析这些样品中的挥发性化合物成分,并比较不同发情状态及不同发情阶段的差异。结果表明,发现40个在所有母牛唾液中都存在的化合物,经过配对分析,从中筛选出23个发情时含量显著高于发情前的化合物(P<0.05),其中,13个化合物(1-戊醇、吡咯、1-己醇、3-己烯-1-醇、苯乙醇、6-甲基-2-庚酮、6-(2,2,2-三氯-1-羟乙基)-1-甲基环己烯和2-戊基呋喃、2-甲基-1-戊醇、2,6,6-三甲基-2-环己烯-1,4-二酮、3-甲基-1-丁醇、二甲基砜、亚磺酰基双甲烷)在所有母牛发情时含量均有升高趋势。这些化合物可作为发情候选标记用于母牛发情鉴定,并且其含量在正常发情和安静发情牛间差异较小,为同时实现正常发情和安静发情鉴定提供了重要参考。

关键词: 荷斯坦母牛, 发情鉴定, 唾液, 信息素

Abstract: This study aimed to provide important reference for the development of silent estrus identification technology based on the fact that male and female cows communicate through sex pheromone. The 25 healthy Holstein cows aged 3 to 5 weeks from Shijiazhuang Tianquan Good Breed Dairy Co. Ltd. were pre-selected, which were 15 to 35 d postpartum, and artificial observation, activity monitoring, rectal examination and B-ultrasound detection and other methods were used to identify the estrus of Holstein cows, and distinguish between normal estrus and silent estrus. The saliva samples of a total of 4 normal estrus and 5 silent estrus cows were collected at different stages of estrus. The Solid Phase Micro-extraction Gas Chromatography-Mass Spectrometry (SPME-GC-MS) was used to identify and analyze the volatile compounds in these saliva samples, and compare the differences between different estrus states and different estrus stages. The results showed that 40 compounds were found in the saliva of all cows, and 23 compounds with significantly higher content during estrus than before estrus (P<0.05) were screened out by paired analysis, among which 13 compounds (1-Pentanol, pyrrole, 1-Hexanol, 3-hexen-1-ol, Phenethyl alcohol, 6-methyl-2-heptanone, 6- (2,2,2-trichloro-1-hydroxyethyl)-1-methyl Cyclohexene and 2-pentylfuran, 2-methyl-1- pentanol,2,6,6-trimethyl-2-neneneba cyclohexene 1,4-dione, 3-methyl-1-butanol, Methylsulfonylmethane, Sulfonyl group bismethane) showed an increasing trend in all cows during estrus. These compounds can be used as estrus candidate markers for cow estrus identification, and their content is small in difference between normal estrus and silent estrus cows, providing important reference for simultaneous identification of normal estrus and silent estrus.

Key words: Holstein cow, estrus identification, saliva, pheromones

中图分类号:

S823.3

董智豪, 时玉新, 郭冠华, 原开敏, 修豪宇, 汪超, 白俊艳, 王栋. 母牛不同发情阶段唾液化合物的比较分析[J]. 畜牧兽医学报, 2023, 54(11): 4636-4652.

DONG Zhihao, SHI Yuxin, GUO Guanhua, YUAN Kaimin, XIU Haoyu, WANG Chao, BAI Junyan, WANG Dong. Comparative Analysis of Salivary Compounds in Different Estrous Stages of Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4636-4652.

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母牛不同发情阶段唾液化合物的比较分析

Comparative Analysis of Salivary Compounds in Different Estrous Stages of Cows

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