两株J亚型禽白血病病毒分离鉴定及全基因序列分析

doi:10.11843/j.issn.0366-6964.2025.12.035

Abstract

Abstract: To investigate the evolutionary trends and molecular characteristics of Avian leukosis virus (ALV), this study collected 1 600 blood samples from a poultry farm in Shanghai. Viruses were isolated by inoculating chicken fibroblast cells (DF-1), followed by subgroup identification through PCR amplification of the gp85 gene. Biological characterization was performed using indirect immunofluorescence assay (IFA) and multi-step growth curves. Coding and non-coding regions of the isolated strains were compared with representative ALV subgroups for sequence alignment and molecular conformation analysis. The results showed that six ALV-J strains were successfully isolated and identified via PCR and IFA. Notably, strains ALV-J-345 and ALV-J-480 exhibited significant sequence divergence, with strain ALV-J-480 demonstrating stronger replication efficiency in DF-1 cells than strain ALV-J-345. Further comparison with other ALV-J strains revealed 46 distinct amino acid variations in the gp85 and gp37 protein between the two isolates, and strain ALV-J-480 displayed lower homology to other ALV-J strains. Additionally, a premature termination codon at position 866 in the pol gene of strain ALV-J-480 truncated the protein by eight amino acids. In non-coding regions, strain ALV-J-345 harbored a 7-nucleotide deletion in the E(XSR) element region of the 3'UTR, whereas strain ALV-J-480 carried a 19 nt nucleotide insertion at positions 358-376 in the 5'UTR. These sequence variations likely contributed to the enhanced replication capacity of strain ALV-J-480, endowing it with a competitive advantage to emerge as a potential epidemic strain in poultry populations. Therefore, this study provides updated molecular insights into ALV-J, which will aid in elucidating its evolutionary dynamics and inform the development of targeted control strategies.

Key words: avian leukosis virus, isolation and identification, gp85, sequence analysis, genetic variation

CLC Number:

S852.659.3

XIA Qiqi, GUI Yaping, LIU Jian, YANG Xianchao, CHEN Qi, SHEN Liping, FENG Guidan, XIANG Meng, XU Ping, TANG Congsheng, WEI Longxing, HONG Yunchao, SHI Huihua, WANG Jian, ZHAO Hongjin. Isolation, Identification, and Whole Gene Sequence Analysis of Two J Subtype Avian Leukemia Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6326-6338.

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Isolation, Identification, and Whole Gene Sequence Analysis of Two J Subtype Avian Leukemia Virus

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