一株牛源变栖克雷伯菌噬菌体的分离鉴定、生物学特性及全基因组分析

doi:10.11843/j.issn.0366-6964.2025.12.038

Abstract

Abstract: This study aimed to isolate a lytic Klebsiella variicola bacteriophage, characterize its biological properties, and conduct whole-genome analysis. The phage was isolated and purified by the double-layer agar culture method from a dairy farm in Sichuan province. Its biological characteristics, including lysis spectrum, thermal stability, acid-base tolerance, optimal infection multiplicity, and one-step growth curve, were determined. Additionally, whole-genome analysis was conducted and phage morphology was observed by transmission electron microscopy (TEM). The results showed that, the isolated lytic Klebsiella variicola bacteriophage vB_Kvc_Y10. The morphology of plaque is round and transparent with clear boundaries, and the TEM shows that Y10 has a short, filamentous tail. The biological results showed that phage Y10 has high host specificity, and is not resistant to high temperatures and acidic environment. Meanwhile, the optimal multiplicity of infection (OMOI) is 0.01. The result of one-step growth curve assay indicates that the latency period is 40 minutes, and the lysis amount is 306. Whole-genome analysis indicates that bacteriophage Y10 belongs to the family of Autographiviridaes and is a dsDNA virus. The complete genome size is 43 281 bp, with a GC content of 56.19%. Among the 51 ORFs, 29 have putative functions and exhibit distinct functional partitions. No tRNA or toxin genes are found in the Y10 genome. Both the phylogenetic tree and ANI analysis indicate that bacteriophage Y10 is closely related to bacteriophage vB_Kpl_K59PH2 (accession number: OY757063). In conclusion, bacteriophage Y10 is a highly specific lytic bacteriophage without virulence genes, presenting potential for the prevention and treatment of bacterial infections.

Key words: Klebsiella variicola bacteriophage, isolation and identification, biological properties, genome-wide analysis

CLC Number:

S852.61

ZENG Yan, YANG Yonggen, WU Zhaomin, ZHANG Jieru, WANG Yin, LUO Yan, YANG Zexiao, CAO Suizhong, YAO Xueping. Isolation and Identification, Biological Properties, and Whole-genome Analysis of a Lytic Bovine Klebsiella variicola Bacteriophage[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6364-6374.

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Isolation and Identification, Biological Properties, and Whole-genome Analysis of a Lytic Bovine Klebsiella variicola Bacteriophage

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