脂多糖诱导奶牛子宫内膜上皮细胞焦亡的分析

doi:10.11843/j.issn.0366-6964.2025.10.042

Abstract

Abstract:

This study aimed to analyze the pyroptosis of endometrial epithelial cells induced by lipopolysaccharide (LPS) in dairy cows. Endometrial epithelial cells of dairy cows were treated with 0.1 μmol·L^-1 LPS. The mRNA transcription levels of related inflammatory factors (IL-1β, Nod-1, TNF-α, IL-18 and IL-6) were detected by real-time fluorescence quantitative PCR (qPCR). The protein levels of related inflammatory factors (IL-1β, IL-6 and TNF-α) were detected by enzyme-linked immunosorbent assay (ELISA). The morphological changes of endometrial epithelial cells of dairy cows were further observed by scanning electron microscopy. The expression levels of pyroptosis-related proteins (NLRP3, IL-18, IL-1β, GSDMD, Cleaved caspase-1 and Cleaved GSDMD) were detected by Western blot. The results showed that the mRNA transcription levels of inflammatory factors (IL-1β, Nod-1, TNF-α, IL-18 and IL-6) in endometrial epithelial cells of dairy cows increased after LPS stimulation for 12 h. Cell swelling and rupture; the expression levels of inflammatory factors IL-1β, IL-6 and TNF-α were increased. The expression of pyroptosis and inflammatory-related proteins (NLRP3, IL-18, IL-1β, GSDMD, Cleaved caspase-1 and Cleaved GSDMD) was significantly enhanced (P < 0.05). LPS stimulation can induce pyroptosis in bovine endometrial epithelial cells.

Key words: cell damage, endometrial epithelial cells of dairy cows, pyroptosis

CLC Number:

S857.23

WANG Zhengyu, FU Chunyu, HUANG Zixuan, GAO Ying, YU Yong, LU Wengeng. Analysis of LPS-induced Pyroptosis of Endometrial Epithelial Cells in Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 5232-5238.

Figures/Tables 5

Table 1

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References 22

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基因Gene	引物Primer	序列(5′→3′) Sequence
β-Actin	Forward	TCCTGCGGCATTCACGAAACTAC
	Reverse	GTGTTGGCGTAGAGGTCCTTGC
IL-1β	Forward	ATGAAGAGCTGCATCCAACA
	Reverse	ATGGAAGACATGTGCGTAGG
TNF-α	Forward	TGACGGGCTTTACCTCATCT
	Reverse	TGATGGCAGACAGGATGTTG
IL-6	Forward	AACGAGTGGGTAAAGAACGC
	Reverse	CTGACCAGAGGAGGGAATGC
IL-18	Forward	CAGATAATGCACCCCAGACCA
	Reverse	TGGTTACGGCCAGACCTCTA
GSDMD	Forward	TGGTGTTGTCCTCCGGAATG
	Reverse	GCGTTTCACTCAGCATGGTC
NLRP3	Forward	ATCTGAAGCTCTGGTTGGTC
	Reverse	GAGTCTCCCAAGGCATTCTCC
Caspase-1	Forward	GTTCCAGGGTGAAGATAATGTTT
	Reverse	TGAAAATCGAACCTTGCGGA

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Analysis of LPS-induced Pyroptosis of Endometrial Epithelial Cells in Dairy Cows

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