利用牛子宫内膜上皮细胞和小鼠子宫内膜炎模型评价胎盘组织源脂质体载药复合物的效果

doi:10.11843/j.issn.0366-6964.2025.10.043

Abstract

Abstract:

This study aimed to evaluate the feasibility of placenta-derived phospholipids as carriers for antibacterial drugs and explore their application in the treatment of endometritis. An in vitroinflammatory model of bovine endometrial epithelial cells and an in vivomodel of endometritis in mice were established using Escherichia coli (E. coli), and the therapeutic effect of gentamicin-placenta lipid complex (L-GEN) was evaluated. Results were as follows: Gentamicin liposomes (L-GEN) were prepared by the film dispersion method; the L-GEN suspension was milky white with a bluish tinge and good fluidity. The particle size of L-GEN was measured by a nanoparticle size and zeta potential analyzer to be 293.7 nm±2.4 nm, with a polydispersity index of 0.21±0.02. No significant change in the particle size of L-GEN was observed within 30 days, indicating the stability of the liposomes. Transcriptome sequencing analysis showed that L-GEN had a smaller impact on bovine endometrial cells compared to the control group. KEGG pathway enrichment analysis revealed that L-GEN mainly enriched in the PPAR signaling pathway, affecting cellular lipid metabolism, reducing cell damage and inflammation, and enhancing antibacterial activity. In vivo experiments found that L-GEN could alleviate the congestion and swelling of the mouse uterus tissue and inflammatory cell infiltration after E. coli perfusion, and the expression levels of inflammatory-related genes IL-2, IL-6, IL-1β, TNF-α, and TLR2 decreased (P≤ 0.05), thereby alleviating endometrial damage and inflammatory response in mice. Through the endometritis cell model and the in vivomodel of endometritis in mice, it was found that the placenta-derived lipid drug delivery complex can effectively alleviate endometrial inflammatory responses and enhance therapeutic effects, providing a reference for the subsequent use of tissue-derived liposomes to carry antibiotics for the treatment of endometritis.

Key words: gentamicin lipid complex, Escherichia coli, endometrial cells, endometritis

CLC Number:

S859.53

ZHOU Jin, ZHUANG Yujie, ZHANG Yalin, XIE Xiaoyu, SUN Mingzhu, ZHANG Xuemei, WANG Xiaolong, ZHANG Zhiping, ZHANG Juntao. Antibacterial Test of Placental Tissue-derived Liposome Drug Complex in Endometritis Model[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 5239-5250.

Figures/Tables 11

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References 19

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引物Primer	产物大小/bp Product size	序列(5′→3′)Sequence
TNF-α	133	F: CTTCTCATTCCTGCTTGTG R: ACTTGGTGGTTTGCTACG
IL-1β	139	F: CCTGGGCTGTCCTGATGAGAG R: TCCACGGGAAAGACACAGGTA
β-actin	102	F: CCTGCGGCATTCACGAAAC R: GGGGGCGCGATGATCTTG
IL-2	125	F: TTGGACCTCTGCGGCATGTT R: CTCAAATGTGTTGTCAGAGCCC
IL-6	132	F: GGCGGATCGGATGTTGTGAT R: GGACCCCAGACAATCGGTTG
TLR2	113	F: TTTGCTCCTGCGAACTCC R: GCCACGCCCACATCATTC
CD36	120	F: GTGTAACTGTCCTCTGTGTCTAAGC R: GCCTCAAGTGCTGGGTCAAATC
PLIN2	127	F: GATGCTGTGACGACTACTGTGAC R: TGCCACTGACCACGGACTTG
SCD	112	F: CTCCGCTCCGCAGTCTCAG R: TGGTGGTGGTGGTTGTGTAGG
HMGCS1	128	F-CACTACAGCCGAGCATATTCCAAG R-AAGTCCTCCACCTCACAGAAGTATC
ANGPTL4	81	F-TCTGGTGGCTGGTGGTTTGG R-CTGCCGCTGCCTTGGAATG

差异表达基因集名称 Differentially expressed gene set name	差异表达基因数目 Number of differentially expressed genes	上调基因数目 Up-regulate the number of genes	下调基因数目 Number of down-regulated genes
E.coli_vs_GEN	4 344	2 093	2 251
E.coli_vs_L	3 476	1 595	1 881
E.coli_vs_L-GEN	3 268	1 517	1 751
GEN vs_L-GEN	70	44	26
GEN_vs_L	101	61	40

基因名称 Gene name	对照组 Control FPKM	L-GEN组 Treat FPKM	对数 log₂ (Treat /Control)	P值 P-value (Control-vs Treat)
CD36	0.156 739	0.497 800	2.196 005 757 505 25	1.09×10^-6
PLIN2	46.520 181	113.105 376	1.244 822 273 767 47	1.27×10^-42
SCD	19.684 074	11.821 800	1.192 551 399 957 22	4.64×10^-26
HMGCS1	21.208 651	16.174 757	1.048 692 230 259 87	4.65×10^-13
ANGPTL4	1.229 391	13.984 678	3.260 825 231 994 90	2.91×10^-45

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Antibacterial Test of Placental Tissue-derived Liposome Drug Complex in Endometritis Model

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