新疆幼畜致泻性大肠埃希菌的毒力基因、耐药性和生物膜的相关性分析

doi:10.11843/j.issn.0366-6964.2025.10.032

Abstract

Abstract:

In recent years, bacterial drug resistance has become an increasingly significant issue threatening animal husbandry and human health. However, the regulatory mechanisms of bacterial drug resistance are extremely complicated. This study aims to analyze the correlations between virulence genes, drug resistance and biofilm formation ability of diarrheagenic Escherichia coli(DEC). Virulence genes, phylogenetic grouping, drug sensitivity testing, biofilm formation ability assay and hemolysis tests were carried out on 76 strains of E. coli from young livestock with diarrhea in Xinjiang. The results showed that 76 strains of DEC included ETEC (n=28), STEC (n=6), EPEC (n=5), ETEC/STEC (n=23), ETEC/EPEC (n=1), ETEC/EPEC/STEC (n=13), mainly belonging to group B1 (40.8%) and group A (28.9%). The resistance rates to amoxicillin, ampicillin, tetracycline and cefotaxime were 53.9%-80.3%, to streptomycin, florfenicol, sulfamethoxazole, ceftriaxone, ceftazidime, gentamicin and ciprofloxacin were 21.1%-47.4%, and to levofloxacin, ampicillin sulbactam, amikacin, fosfomycin and polymyxin B were 1.3%-15.8%. About 73.7% of the isolates had strong biofilm formation ability, 17.1% had moderate biofilm formation ability, 7.9% had weak biofilm formation ability, and 1.3% had no biofilm formation ability. Fourteen strains showed β-hemolysis. Strong biofilm-forming strains were more common among DEC strains carrying STb, stx1, hly and eae (P＜0.001). The biofilm formation ability was related to hemolysis (P＜0.05), not related to multidrug resistance (P>0.05), significantly related to ceftazidime resistance (P＜0.001), and related to ampicillin-sulbactam (P=0.027 9, P＜0.05). The research results indicated that the types of DEC in young livestock with diarrhea in Xinjiang were complex, and most strains had the biofilm formation ability. The biofilm formation ability was related to virulence genes and hemolysis, and the related to resistance to cefotaxime and ampicillin sulbactam. The biofilm formation ability might be associated with the failure of antibiotic treatment and persistent infection. This study provides a reference for the prevention and treatment of diarrhea in young livestock.

Key words: diarrheagenic Escherichia coli, biofilm, drug resistance, virulence genes, young livestock

CLC Number:

S852.612

BAI Yixin, ZHANG Mengfei, Dinala·Yeerboli , WANG Lei, JIN Wanjing, HUANG Ying, XIE Jinxin, ZHENG Xiaofeng, WANG Meiling, WANG Chuanfeng, SU Zhanqiang, ZHANG Wei, TONG Panpan. Analysis of Virulence Genes, Drug Resistance and Biofilm of Diarrheagenic Escherichia coli from Young Livestock in Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 5125-5136.

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动物 Animal	DEC类型(数量) DEC type (No.)	进化分群(数量) Phylo-group (No.)	溶血菌株(数量) Hemolytic isolates (No.)	生物膜形成能力(数量) Biofilm formation (No.)
猪 Pig	STEC (1)	B1 (1)	ETEC (8), ETEC/STEC (4), STEC (1) ETEC/STEC/EPEC (1)	强(20) Strong (20)
	ETEC (12)	A (6), B1 (5), C (1)
	ETEC/STEC (6)	A (2), B1 (2), C (2)
	ETEC/STEC/EPEC (1)	E (1)
牛 Cattle	STEC (2)	A (2)	0	强(18), 中(13), 弱(6), 无(1) Strong (18), medium (13), weak (6) and non (1)
	ETEC (9)	A (1), B1 (2), C (2), E (1), unknow (3)
	EPEC (1)	A (1)
	ETEC/STEC (13)	A (5), B1 (5), C (1), E (1), unknow (1)
	ETEC/EPEC (1)	A (1)
	ETEC/STEC/EPEC (12)	A (3), B1 (8), E (1)
羊 Sheep	STEC (3)	A (1), C (1), unknow (1)	0	强(18) Strong (18)
	ETEC (7)	B1 (4), C (2), unknow (1)
	EPEC (4)	B1 (2), C (1), unknow(1)
	ETEC/STEC (4)	B1 (2), C (2)
合计 Total	ETEC (28), ETEC/STEC (23), ETEC/EPEC/STEC (13), STEC (6), EPEC (5), ETEC/EPEC (1),	B1 (31), A (22), C (12), E (4), unknow (7)	ETEC (8), ETEC/STEC (4), STEC (1) ETEC/STEC/EPEC (1)	强(56), 中(13), 弱(6), 无(1) Strong (56), medium (13), weak (6) and non (1)

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Analysis of Virulence Genes, Drug Resistance and Biofilm of Diarrheagenic Escherichia coli from Young Livestock in Xinjiang

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