加味枳术散对断奶仔兔肠黏膜屏障和抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2024.12.044

Abstract

Abstract:

The study aimed to investigate the effects of Modified Zhizhu Powder on the ileal intestinal mucosal barrier of weaned rabbits, with the goal of providing new ideas for the prevention and treatment of diarrhea diseases in weaned rabbits. Ninety 35-day-old New Zealand white rabbits, with similar body weights, were randomly divided into 5 groups, based on the principle of 50/50 male and female. Rabbits in control group were fed a basal diet, Enrofloxacin group, low-dose group, medium-dose group and high-dose group were fed the basal diet supplemented with 0.2% Enrofloxacin powder and 0.1%, 0.3% and 0.5% Modified Zhizhu Powder, respectively. The pre-test lasted for 7 days, and the formal test lasted for 14 days. The integrity of the intestinal barrier and its antioxidant capacity were measured after the experiment. The results showed that adding Modified Zhizhu Powder could significantly reduce the intestinal pathological tissue score (P < 0.05). Compared with the blank group, the other treatment groups all significantly increased the relative expression level of ZO2 (zonula occludens protein 2) mRNA in the ileum (P < 0.001). The high-dose group significantly increased the mRNA levels of Occludin, claudin, ZO1 (zonula occludens protein 1), ZO2, andJAM2 (junctional adhesion molecule 2) (P < 0.001). Modified Zhizhu Powder significantly increased the expression of MUC2 (Mucin 2) protein in the ileum (P < 0.05) and the high-dose group significantly reduced MPO (myeloperoxidase) activity (P < 0.01). Modified Zhizhu Powder high-doce group could significantly enhance the secretion of sIgA, reduce the levels of pro-inflammatory cytokinesIL-1β, IL-2, IL-6, andTNF-α, and increase the levels of anti-inflammatory cytokinesIL-10 andTGF-β. Modified Zhizhu Powder could also improve the activity of antioxidant enzymes (SOD, GSH, T-AOC, CAT). In conclusion, the Modified Zhizhu Powder could effectively improve the morphology and structure of the ileum of weaned rabbits, strengthen the tight connection between intestinal cells, maintain the permeability of intestinal mucosa, reduce the inflammatory response of the intestinal tract, enhance the function of the intestinal mucosal barrier, and play an antioxidant role, which can have a positive effect on the use of substitute antibodies.

Key words: weaning diarrhea of young rabbits, Modified Zhizhu Powder, intestinal mucosal barrier, inflammatory response, antioxidation

CLC Number:

S853.74

JIN Ruwen, WANG Yingjie, JIANG Quanxing, LIU Tianqiang, DENG Yang, LUO Jie, ZHAO Ling, YE Gang, SHI Fei, LI Yinglun, TANG Huaqiao. Effects of Modified Zhizhu Powder on Intestinal Mucosal Barrier and Antioxidant Capacity of Weaned Rabbits[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5825-5838.

Figures/Tables 10

Table 1

Table 2

Table 3

Table 4

Table 5

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

References 51

1	CARABAÑO R , BADIOLA I , CHAMORRO S , et al. New trends in rabbit feeding: Influence of nutrition on intestinal health[J]. Span J Agric Res, 2008, 6 (S1): 15- 25.
2	李亚力. 乳酸菌对幼龄獭兔生产性能与免疫性能的影响[D]. 杨凌: 西北农林科技大学, 2013.
	LI Y L. Effects of lactic acid bacteria on growth performance and immune capability of juvenile rabbits[D]. Yangling: Northwest A & F University, 2013. (in Chinese)
3	CAMPBELL J M , CRENSHAW J D , POLO J . The biological stress of early weaned piglets[J]. J Anim Sci Biotechnol, 2013, 4 (1): 19. doi: 10.1186/2049-1891-4-19
4	CHEN H , HU H Y , CHEN D W , et al. Dietary pectic oligosaccharide administration improves growth performance and immunity in weaned pigs infected by rotavirus[J]. J Agric Food Chem, 2017, 65 (14): 2923- 2929. doi: 10.1021/acs.jafc.7b00039
5	WU Y , TANG L , WANG B , et al. The role of autophagy in maintaining intestinal mucosal barrier[J]. J Cell Physiol, 2019, 234 (11): 19406- 19419. doi: 10.1002/jcp.28722
6	MARTENS E C , NEUMANN M , DESAI M S . Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier[J]. Nat Rev Microbiol, 2018, 16 (8): 457- 470. doi: 10.1038/s41579-018-0036-x
7	GUSTAFSSON J K , JOHANSSON M E V . The role of goblet cells and mucus in intestinal homeostasis[J]. Nat Rev Gastroenterol Hepatol, 2022, 19 (12): 785- 803. doi: 10.1038/s41575-022-00675-x
8	XU Q Q , JIAN H F , ZHAO W Y , et al. Early weaning stress induces intestinal microbiota disturbance, mucosal barrier dysfunction and inflammation response activation in pigeon squabs[J]. Front Microbiol, 2022, 13, 877866. doi: 10.3389/fmicb.2022.877866
9	WEN Z S , DU M , TANG Z , et al. Low molecular seleno-aminopolysaccharides protect the intestinal mucosal barrier of rats under weaning stress[J]. Int J Mol Sci, 2019, 20 (22): 5727. doi: 10.3390/ijms20225727
10	WESTRÖM B , ARÉVALO SUREDA E , PIERZYNOWSKA K , et al. The immature gut barrier and its importance in establishing immunity in newborn mammals[J]. Front Immunol, 2020, 11, 1153. doi: 10.3389/fimmu.2020.01153
11	中华中医药学会脾胃病分会. 泄泻中医诊疗专家共识意见(2017)[J]. 中医杂志, 2017, 58 (14): 1256- 1260.
	Spleen and Stomach Disease Branch of the Chinese Association of Traditional Chinese Medicine . Consensus opinion of experts on diagnosis and treatment of diarrhea in traditional Chinese medicine (2017)[J]. Journal of Traditional Chinese Medicine, 2017, 58 (14): 1256- 1260.
12	薛辉, 王庆其. 结合运气学说辨治泄泻证及医案剖析[J]. 中国中医基础医学杂志, 2010, 16 (2): 103- 104.
	XUE H , WANG Q Q . Diagnosis and treatment combined with Yun-Qi theory and analysis of medical records of diarrhea[J]. Chinese Journal of Basic Medicine in Traditional Chinese Medicine, 2010, 16 (2): 103- 104.
13	史焱, 李君, 傅海燕. 《医学汇函》引用《古今医鉴》版本考[J]. 中国中医基础医学杂志, 2016, 22 (5): 602- 606.
	SHI Y , LI J , FU H Y . Edition reference of Yi Xue Han Hui quoting Gu Jin Yi Jian[J]. Chinese Journal of Basic Medicine in Traditional Chinese Medicine, 2016, 22 (5): 602- 606.
14	杨东亮, 赵翠苗. 枳术散热敷腹部治疗结肠癌术后胃肠功能紊乱临床观察[J]. 中医临床研究, 2016, 8 (5): 73- 74. doi: 10.3969/j.issn.1674-7860.2016.05.044
	YANG D L , ZHAO C M . Clinical observation on treating cladding gastrointestinal dysfunction after abdominal surgery colon cancer[J]. Clinical Journal of Chinese Medicine, 2016, 8 (5): 73- 74. doi: 10.3969/j.issn.1674-7860.2016.05.044
15	张均华. 橘皮枳术散加减预防犊泄泻[J]. 中兽医学杂志, 1986, (4): 49.
	ZHANG J H . Modified orange peel Zhizhu powder to prevent calf diarrhea[J]. Chinese Journal of Traditional Veterinary Science, 1986, (4): 49.
16	李桂甫. 肉兔饲料及饲养规范探讨[J]. 中国养兔, 2019, (2): 43- 44. doi: 10.3969/j.issn.1005-6327.2019.02.016
	LI G F . Discussion on meat rabbit feed and feeding norms[J]. Chinese Journal of Rabbit Farming, 2019, (2): 43- 44. doi: 10.3969/j.issn.1005-6327.2019.02.016
17	ZENG Y D , WANG Z R , ZOU T D , et al. Bacteriophage as an alternative to antibiotics promotes growth performance by regulating intestinal inflammation, intestinal barrier function and gut microbiota in weaned piglets[J]. Front Vet Sci, 2021, 8, 623899. doi: 10.3389/fvets.2021.623899
18	彭婧, 肖传斌, 王春秀. 板蓝根多糖对少乳仔鼠空肠形态的影响[J]. 养殖与饲料, 2021, 20 (9): 47- 50. doi: 10.3969/j.issn.1671-427X.2021.09.015
	PENG J , XIAO C B , WANG C X . Effects of Isatis indigotica polysaccharide on the morphology of jejunum in young mice[J]. Animals Breeding and Feed, 2021, 20 (9): 47- 50. doi: 10.3969/j.issn.1671-427X.2021.09.015
19	BEUTEL O , MARASPINI R , POMBO-GARCÍA K , et al. Phase separation of zonula Occludens proteins drives formation of tight junctions[J]. Cell, 2019, 179 (4): 923- 936. e11. doi: 10.1016/j.cell.2019.10.011
20	JIN Y H , ZHAI Z A , JIA H , et al. Kaempferol attenuates diquat-induced oxidative damage and apoptosis in intestinal porcine epithelial cells[J]. Food Funct, 2021, 12 (15): 6889- 6899. doi: 10.1039/D1FO00402F
21	许露, 张曼, 钱凯, 等. 白术/防风药对对Caco-2细胞屏障功能的协同保护作用及机制研究[J]. 河南医学研究, 2018, 27 (5): 769- 772. doi: 10.3969/j.issn.1004-437X.2018.05.001
	XU L , ZHANG M , QIAN K , et al. Study of the synergistic effects of Atractylodes macrocephala and Saposhnikovia divaricate on Caco-2 cell and it's mechanism[J]. Henan Medical Research, 2018, 27 (5): 769- 772. doi: 10.3969/j.issn.1004-437X.2018.05.001
22	孙必强, 周英, 刘卫东, 等. 不同剂型七味白术散对腹泻小鼠肠道菌群失调和肠粘膜紧密连接蛋白的影响[J]. 时珍国医国药, 2015, 26 (12): 2835- 2837.
	SUN B Q , ZHOU Y , LIU W D , et al. Effects of different formulated Qiwei Baizhu powder on intestinal flora and tight junction protein in diarrhea mice[J]. Lishizhen Medicine and Materia Medica Research, 2015, 26 (12): 2835- 2837.
23	胡红莲, 高民. 肠道屏障功能及其评价指标的研究进展[J]. 中国畜牧杂志, 2012, 48 (17): 78- 82.
	HU H L , GAO M . Research advance in intestinal barrier function and evaluation index[J]. Chinese Journal of Animal Science, 2012, 48 (17): 78- 82.
24	CHELAKKOT C , GHIM J , RYU S H . Mechanisms regulating intestinal barrier integrity and its pathological implications[J]. Exp Mol Med, 2018, 50 (8): 1- 9.
25	汪荣泉, 房殿春, 罗元辉, 等. 消化道癌患者胃肠道粘膜中MUC2和MUC3基因的表达[J]. 基础医学与临床, 2000, 20 (5): 421- 424. doi: 10.3969/j.issn.1001-6325.2000.05.011
	WANG R Q , FANG D C , LUO Y H , et al. Expression of MUC2 and UUC3 genes in the gastrointestinal mucosas of patients suffered from gastrointestinal neoplasms[J]. Basic and Clinical Medicine, 2000, 20 (5): 421- 424. doi: 10.3969/j.issn.1001-6325.2000.05.011
26	MANTLE M , HUSAR S D . Binding of Yersinia enterocolitica to purified, native small intestinal mucins from rabbits and humans involves interactions with the mucin carbohydrate moiety[J]. Infect Immun, 1994, 62 (4): 1219- 1227. doi: 10.1128/iai.62.4.1219-1227.1994
27	HECZKO U , ABE A , FINLAY B B . In vivo interactions of rabbit enteropathogenic Escherichia coli O103 with its host: an electron microscopic and histopathologic study[J]. Microbes Infect, 2000, 2 (1): 5- 16. doi: 10.1016/S1286-4579(00)00291-4
28	MANTLE M , BASARABA L , PEACOCK S C , et al. Binding of Yersinia enterocolitica to rabbit intestinal brush border membranes, mucus, and mucin[J]. Infect Immun, 1989, 57 (11): 3292- 3299. doi: 10.1128/iai.57.11.3292-3299.1989
29	朱春洋, 赵鲁卿, 赵静怡, 等. 四逆散对FD大鼠十二指肠杯状细胞及MUC2的影响[J]. 世界中医药, 2020, 15 (11): 1575-1578, 1584. doi: 10.3969/j.issn.1673-7202.2020.11.012
	ZHU C Y , ZHAO L Q , ZHAO J Y , et al. Effects of Sini powder on duodenum goblet cells and MUC2[J]. World Chinese Medicine, 2020, 15 (11): 1575-1578, 1584. doi: 10.3969/j.issn.1673-7202.2020.11.012
30	NDREPEPA G . Myeloperoxidase-A bridge linking inflammation and oxidative stress with cardiovascular disease[J]. Clin Chim Acta, 2019, 493, 36- 51. doi: 10.1016/j.cca.2019.02.022
31	CAO Z H , CHENG G J . Recombinant myeloperoxidase as a new class of antimicrobial agents[J]. Microbiol Spectr, 2022, 10 (1): e0052221. doi: 10.1128/spectrum.00522-21
32	SIRAKI A G . The many roles of myeloperoxidase: from inflammation and immunity to biomarkers, drug metabolism and drug discovery[J]. Redox Biol, 2021, 46, 102109. doi: 10.1016/j.redox.2021.102109
33	AGRAWAL B . Heterologous immunity: role in natural and vaccine-induced resistance to infections[J]. Front Immunol, 2019, 10, 2631. doi: 10.3389/fimmu.2019.02631
34	WISE R D , LICHTER E A , DRAY S . Presence in rabbit colostrum of IgA autoantibodies to a gastric antigen[J]. J Immunol, 1971, 107 (1): 47- 55. doi: 10.4049/jimmunol.107.1.47
35	LAMM M E . Interaction of antigens and antibodies at mucosal surfaces[J]. Annu Rev Microbiol, 1997, 51, 311- 340. doi: 10.1146/annurev.micro.51.1.311
36	YANG Y , PALM N W . Immunoglobulin A and the microbiome[J]. Curr Opin Microbiol, 2020, 56, 89- 96. doi: 10.1016/j.mib.2020.08.003
37	DE FAYS C , CARLIER F M , GOHY S , et al. Secretory immunoglobulin a immunity in chronic obstructive respiratory diseases[J]. Cells, 2022, 11 (8): 1324. doi: 10.3390/cells11081324
38	CORTHESY B . Multi-faceted functions of secretory IgA at mucosal surfaces[J]. Front Immunol, 2013, 4, 185.
39	孙必强, 伍参荣, 周英, 等. 不同剂型七味白术散对肠道菌群失调小鼠小肠黏膜超微结构和sIgA的影响[J]. 中国微生态学杂志, 2016, 28 (2): 125-1288, 137.
	SUN B Q , WU C R , ZHOU Y , et al. Effects of different formulations of Qiwei Baizhu powder on the ultrastructure of small intestinal mucosa and sIgA in mice with intestinal dysbacteriosis[J]. Chinese Journal of Microecology, 2016, 28 (2): 125-1288, 137.
40	BRANCA J J V , GULISANO M , NICOLETTI C . Intestinal epithelial barrier functions in ageing[J]. Ageing Res Rev, 2019, 54, 100938. doi: 10.1016/j.arr.2019.100938
41	韩渤, 刘玥宏, 仇志强, 等. SIgA表达功能及其在肠道疾病的作用[J]. 世界华人消化杂志, 2017, 25 (19): 1757- 1763.
	HAN B , LIU Y H , QIU Z Q , et al. Secretory immunoglobulin A and intestinal diseases[J]. World Chinese Journal of Digestology, 2017, 25 (19): 1757- 1763.
42	罗治彬. 肠道粘膜SIgA免疫系统的研究进展[J]. 细胞与分子免疫学杂志, 1997, 13 (S2): 38- 41.
	LUO Z B . Research progress of intestinal mucosal SIgA immune system[J]. Journal of Cellular and Molecular Immunology, 1997, 13 (S2): 38- 41.
43	FIORANELLI M , ROCCIA M G , FLAVIN D , et al. Regulation of inflammatory reaction in health and disease[J]. Int J Mol Sci, 2021, 22 (10): 5277. doi: 10.3390/ijms22105277
44	MALOY K J , POWRIE F . Intestinal homeostasis and its breakdown in inflammatory bowel disease[J]. Nature, 2011, 474 (7351): 298- 306.
45	BURGE K , GUNASEKARAN A , ECKERT J , et al. Curcumin and intestinal inflammatory diseases: molecular mechanisms of protection[J]. Int J Mol Sci, 2019, 20 (8): 1912.
46	NIKI E . Assessment of antioxidant capacity in vitro and in vivo[J]. Free Radical Biol Med, 2010, 49 (4): 503- 515.
47	王柏森. 绿原酸通过抑制TLR4/MAPK/NFκB通路激活自噬抑制炎症和氧化应激[D]. 长春: 吉林大学, 2021.
	WANG B S. Chlorogenic acid inhibits inflammation and oxidative stress by inhibiting TLR4/MAPK/NFκB pathway and activating autophagy[D]. Changchun: Jilin University, 2021. (in Chinese)
48	OLSVIK P A , KRISTENSEN T , WAAGBØR , et al. mRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2005, 141 (3): 314- 323.
49	SAKAMOTO T , IMAI H . Hydrogen peroxide produced by superoxide dismutase SOD-2 activates sperm in Caenorhabditis elegans[J]. J Biol Chem, 2017, 292 (36): 14804- 14813.
50	GOYAL M M , BASAK A . Human catalase: looking for complete identity[J]. Protein Cell, 2010, 1 (10): 888- 897.
51	TANG Q , SU Y W , XIAN C J . Determining oxidative damage by lipid peroxidation assay in rat serum[J]. Bio Protoc, 2019, 9 (12): e3263.

原料 Ingredient	含量 Content	营养水平² Nutrient level	含量 Content
二级玉米 Two gard corn	12	消化能/(MJ·kg^-1) DE	12.65
豆粕 Soybean meal	5.5	总灰分 Total ash	8.63
膨化大豆 Expanded soybean	2.5	粗蛋白 Crude protein	15.48
玉米胚芽粕 Corn germ meal	12	粗脂肪 Crude fat	4.29
菜籽粕 Rapeseed meal	6	粗纤维 Crude fiber	13.76
苜蓿草颗粒 Alfalfa pellets	22.5	钙 Ca	1.02
统糠 Chaff	13	磷 P	0.51
小麦次粉 Wheat middlings	5	赖氨酸 Lysine	0.79
小麦麸皮 Wheat bran	17	蛋氨酸 Methionine	0.33
302肉兔核心料¹ 302 Meat rabbit core material	2	半胱氨酸 Cystine	0.21
石粉 Limestone	1.1
磷酸氢钙 Ca(HCO₃)₂	0.7
大豆油 Soybean oil	0.7
合计 Total	100

组别 Group	日粮组成 Diet composition	试验动物数量/只 Number of experimental animals
空白组 Control group	基础饲粮	18
恩诺沙星组 Enrofloxacin group	基础日粮+0.2%恩诺沙星粉剂	18
低剂量组 Low-dose group	基础日粮+0.1%加味枳术散粉剂	18
中剂量组 Medium-dose group	基础日粮+0.3%加味枳术散粉剂	18
高剂量组 High-dose group	基础日粮+0.5%加味枳术散粉剂	18

评分 Score	病变情况 Pathological condition
评分 Score	黏膜上皮 Mucosal epithelium	固有层 Proper layer
0	未见脱落坏死	绒毛结构清晰
1	无	毛细管充轻微血
2	上皮纹状缘萎缩消失	毛细管充血，炎细胞浸润
3	黏膜上皮细胞脱落坏死	脂肪细胞增生，炎细胞浸润，形成空泡
4	上皮细胞脱落坏死，较重	炎细胞浸润明显
5	绒毛结构破坏，上皮细胞大面积脱落坏死	脂肪空泡较多，炎细胞浸润明显

组分名称 Constituent	体积/μL Volume
cDNA	2
上游引物 Forwad primer	0.5
下游引物 Reverse primer	0.5
SYBR Green Master Mix	5
ddH₂O	2
合计 Total	10

引物名称 Primer name	登录号 Accession No.	引物序列(5′→3′) Primer sequence(5′→3′)
GAPDH	NM_001082253	F：TCGGAGTGAACGGATTTGGC R：GCCGTGGGTGGAATCATAC
ZO2	XM_017341711	F：GAGGAGAGACGCCAGCA R：GGAGGACGTGAACGGAGT
ZO1	XM_017348360	F：TTTCGTGTTGTGGATACCTT R：TGTATACTGCACACTGG
JAM2	XM_017346699	F：TGATAGATTTCAGTATACGCATC R：GGCACTAACTTCACAACGA
Claudin	XM_017347162	F：GTTGCCAGTTCCTCTTACCTT R：AGATACAGGCACAAAGTCCA
Occludin	XM_017344772	F：TGTTTATTGCCACCATTGTCT R：GTGAGCCATACATAGATCCAG
IL-1β	NM_001082201	F：CAAGAGGCACAACAGATCGC R：CGGCCTCAAAGAACAGGTCA
TNF-α	XM_008262537	F：CACTTCAGGGTGATCGGC R：TGCGGGTTTGCTACTACG
IL-2	NM_001163180	F：CATCATCTCAGCATCGAGTTCA R：TGAAGCCATTTTAGAGCCCCT
IL-6	NM_001082064	F：ACCTGCCTGCTGAGAATCAC R：TCGTCACTCCTGAACTTGGC
IL-10	NM_001082045	F：TCCCTCTTACCCTCTGGAGC R：AAGGGGTTGAAAATCGAGGTCA
TGF-β11	XM_008249704	F：CTGGAACGGGCTCAACATCT R：CAGGTCCTTGCGGAAGTCAA

Effects of Modified Zhizhu Powder on Intestinal Mucosal Barrier and Antioxidant Capacity of Weaned Rabbits

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 51

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Xiaofeng, QIN Xiaowei, LÜ Lihua. Protective Effect of a Derivative of MNQ Against LPS-Induced Inflammatory Injury in Bovine Ovarian Follicular Granulosa Cells in Vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2032-2041.
[2]	DAI Fan, LIU Zhanyou, ZHANG Xuyang, LI Wu. Aconitate Decarboxylase 1 Could Regulate the Inflammatory Response Caused by BCG [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1696-1706.
[3]	Yue HU, Ting YI, Jiayun QIAO, Yupeng LI, Haihua LI. Establishment and Molecular Mechanism Study of Diquat Induced Oxidative Damage Model in Broilers Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(11): 5101-5113.
[4]	Hui ZHANG, Doukun LU, Yiqiu ZHANG, Gang ZHAO, Yingyu CHEN, Xi CHEN, Changmin HU, Aizhen GUO. Establishment of Cattle Precision-Cut Lung Tissue Slices (PCLS) Models Infected with Mycoplasma bovis in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4638-4645.
[5]	Yongqing LIU, Gang ZHANG, Yanling XIONG, Zhongxin SUN, Fan GAO, Ting LIU, Hui LI. Effects of Heat Stress on Duodenal Mucosal Structure, HIF-1 and Its Related Protein Expression in Congjiang Xiang Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4690-4699.
[6]	ZHAO Yangfei, YU Yanghuan, WANG Jinming, ZHANG Jianhai, SUN Zilong, NIU Ruiyan, WANG Jundong. Effects of IL-17A Knockout on Fluoride-Induced Hepatic Inflammation and Hepatocyte Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3108-3117.
[7]	HAN Lulu, HAN Deping, ZHAO Qinan, DIAO Qiyu, CUI Kai. Research Progress of Intestinal Injury in Young Farm Animals under Stress Mediated by miRNA [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 877-888.
[8]	XUE Linli, SUN Rui, HAO Xiaojing, CAO Xiaorui, WANG Haidong, LU Jiayin. The Promoting Effect Analysis of Danshensu on Skeletal Muscle Repair and Regeneration after Skeletal Muscle Injury in Mice based on a Mouse Skeletal Muscle Injury Model [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5252-5263.
[9]	LIU Weiwei, ZHANG Yuxin, LI Xiumei, WANG Xiumin, ZHOU Weiwei, DAI Xiaofeng. Mechanism of Mori Folium in Improving Antioxidative Function of Chicken Based on Network Pharmacology [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1958-1970.
[10]	ZHANG Pengguang, YAN Enfa, WANG Liqi, MA Chenghong, ZHANG Xin, YIN Jingdong. Effects of Dietary Supplementation of L-malic Acid on Inflammatory Response and Intestinal Health Status in Weaned Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4306-4314.
[11]	WANG Meng, YANG Chaoqun, WU Silin, TAN Jianbing, DU Xinze, LI Zhenxing, ZAN Linsen, YANG Wucai. Impact of Lycopene on Semen Cryopreservation and Fresh Semen Quality of Qinchuan Bull [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4507-4517.
[12]	HUANG Xiaoyu, YANG Qiaoli, YAN Zunqiang, WANG Pengfei, SHI Hairen, GUN Shuangbao. Characterization of circRNA Expression Profiles Involved in Intestines of Clostridium Perfringens Type C-infected Diarrheal Piglet [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4058-4070.
[13]	XIE Zhiming, WU Chunmei, CHEN Shaoyuan, WANG Zhao, WANG Yan. Study on Bupleurum Polysaccharide Reduces Lead-induced Liver and Kidney Injury in Mice by Inhibiting Oxidative Stress and Inflammation [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2660-2672.
[14]	LIU Yankun, LIN Yan, ZHU Weiyun. Advances in the Bacteriophage Translocation and the Effect of Bacteriophage on the Immunity [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 588-595.
[15]	ZHU Bin, LIU Xiaoqian, LIU Ying, JI Xiaoxia, ZHANG Yuanshu. Angiotensin-converting Enzyme 2 on Ang Ⅱ-induced Mitigation of Inflammatory Injury in Bovine Mammary Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1458-1465.