柴胡多糖通过抑制氧化应激和炎症反应减轻铅诱导小鼠肝肾损伤的研究

doi:10.11843/j.issn.0366-6964.2021.09.030

Abstract

Abstract: This study aimed to investigate the effect of Bupleurum polysaccharide on lead-induced liver and kidney damage in mice. One hundred and fifty SPF male healthy Kunming mice were selected for the experiment. After two weeks of adaptive feeding, they were randomly divided into 5 groups, namely the control group, the lead treatment group, the lead treatment + Bupleurum polysaccharide low, medium and high dose groups (100, 200 and 400 mg·kg^-1). Establishment of the poisoning model:the mice drink lead water (0.5% lead acetate) every day for 4 consecutive weeks. After the modeling was completed, the test group was given Bupleurum polysaccharide once daily for 2 weeks. The lead content and body mass in the blood and tissues of mice were measured, and the antioxidant enzyme activity, Lipid peroxidation level and inflammatory factors were detected and analyzed, and the relative expression of oxidative damage and inflammatory response related factors mRNA was analyzed from the molecular level. The results showed that compared with the lead-treated group, Bupleurum polysaccharides can inhibit the decreasing body weight and the increase in the proportion of liver and kidney in lead-poisoned mice. The addition of Bupleurum polysaccharides reduced the lead concentration in the blood and tissues of mice, but the effect was no significant (P>0.05); compared with the lead-treated group, Bupleurum polysaccharide can significantly increase the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and peroxidase (CAT) activity in the liver and kidney tissues of lead-exposed mice (P<0.05), and reduce malondialdehyde (MDA) contents; Bupleurum polysaccharides can significantly reduce the lead-induced increase in alanine transferase (ALT) and aspartate transferase (AST) activities in the serum and liver tissues of mice, and the mice serum urea nitrogen (BUN) and creatinine (Scr) level decreased, and the tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and myeloperoxidase (MPO) activities in liver and kidney tissues of mice significantly decreased (P<0.05); Bupleurum polysaccharide can significantly increase the expression of Nrf2 signaling pathway, increase the mRNA levels of HO-1, Cu/Zn-SOD, Mn-SOD, CAT, and reduce the mRNA level of Keap1. In addition, Bupleurum polysaccharide can significantly reduce the increase of mRNA levels of inflammation factors (NF-κB, IL-1β and TNF-α) in lead-induced mice liver and kidney (P<0.05). The results showed that low-dose, middle-dose and high-dose of Bupleurum polysaccharides can alleviate lead-induced lipid peroxidation and inflammation in mice, and have protective effects on liver and kidney damage in mice. Among them, the most significant effect of Bupleurum polysaccharides high-dose group on liver and kidney tissue damage in mice.

Key words: Bupleurum polysaccharide, mice, lead, oxidative stress, inflammatory response, liver and kidney injury

CLC Number:

XIE Zhiming, WU Chunmei, CHEN Shaoyuan, WANG Zhao, WANG Yan. Study on Bupleurum Polysaccharide Reduces Lead-induced Liver and Kidney Injury in Mice by Inhibiting Oxidative Stress and Inflammation[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2660-2672.

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Study on Bupleurum Polysaccharide Reduces Lead-induced Liver and Kidney Injury in Mice by Inhibiting Oxidative Stress and Inflammation

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