枯草芽孢杆菌制剂对肉牛生产性能、瘤胃发酵、血液生化及免疫指标的影响

doi:10.11843/j.issn.0366-6964.2024.12.022

Abstract

Abstract:

The aim of this experiment was to study the effects of Bacillus subtilis preparation on rumen fermentation, performance, blood biochemistry, immune indicators, and meat quality in beef cattle. A completely randomized design was employed, involving 48 Simmental beef cattle in similar physical condition during the fattening period. The cattle were randomly divided into four groups: control group (fed with basic diet, CON), low-dose group (Bacillus subtilis preparation added to the basic diet at 0.3 g·kg^-1 DM, LAL), medium-dose group (Bacillus subtilis preparation added to the basic diet at 0.45 g·kg^-1 DM, LAM), and high-dose group (Bacillus subtilis preparation added to the basic diet at 0.6 g·kg^-1 DM, LAH). Each group had 4 replicates, with 3 cattle in each replicate. The pre-trial period lasted for 10 days, and the formal period lasted for 90 days. The results showed as follows: 1) Adding Bacillus subtilis preparation to the diet did not significantly affect rumen fluid pH, rumen fluid ammonia nitrogen (NH₃-N), dry matter intake (DMI), body measurements, slaughter performance, and meat quality of the experimental cattle (P>0.05). 2)The serum total antioxidant capacity (T-AOC) in the LAL group was significantly higher than that in the CON group (P < 0.05). 3) Compared to the CON group, the molar concentration of butyrate in rumen fluid was significantly lower in LAL and LAM groups (P < 0.05). Additionally, compared to CON group, the ratio of acetate/propionate in rumen fluid in the LAL and LAM groups, total protein (TP) and globulin (GLB) content in blood of the LAM and LAH groups, as well as the activity of alanine transaminase (ALT) and aspartate transaminase (AST) in blood of the LAM and LAH groups, were significantly higher (P < 0.05). 4)Compared to the CON group, the LAH group showed a significant decrease in feed/gain ratio (F/G) (P < 0.05), and the LAH group exhibited significantly higher average daily gain (ADG), triglyceride (TG) levels in blood, haptoglobin (Hp), and serum amyloid A (SAA) compared to the CON group (P < 0.05). In conclusion, Bacillus subtilis preparation can improve the rumen environment, blood antioxidant function, and immune function in beef cattle to varying degrees. The highest weight gain efficiency in cattle was observed when the addition rate was 0.6 g·kg^-1 DM.

Key words: Bacillus subtilis, beef cattle, fermentation parameters, performance, meat quality

CLC Number:

S823.5

WU Jiang, WAN Fachun, LIU Lei, SHEN Weijun, LAN Xinyi, WANG Zuo. Effects of Bacillus Subtilis Preparations on Performance, Ruminal Fermentation, Blood Biochemical and Immune Indices of Beef Cattle[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5575-5589.

Figures/Tables 6

Table 1

Table 2

Table 3

Table 4

Table 5

Effect of Bacillus subtilis preparation on blood biochemical indexes of fattening beef cattle"

项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	P值 P-value
项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	Treat	L	Q
谷丙转氨酶/(U·L^－1) ALT	2.75^b	1.92^b	4.43^a	4.06^a	1.437	< 0.001	0.003	0.013
谷草转氨酶/(U·L^－1) AST	5.08^c	4.80^c	8.50^a	6.77^b	2.326	< 0.001	0.003	0.007
胆碱酯酶/(U·L^－1) CHE	76.40	65.13	84.00	71.63	17.218	0.174	0.872	0.984
尿素/(mmol·L^－1) BUN	3.15	3.60	3.19	3.32	0.449	0.184	0.879	0.625
葡萄糖/(mmol·L^－1) GLU	8.08	7.96	8.67	8.84	1.352	0.517	0.174	0.384
总蛋白/(g·L^－1) TP	81.27^c	83.68^bc	89.34^ab	94.21^a	7.734	0.001	< 0.001	< 0.001
白蛋白/(g·L^－1) ALB	35.48	35.56	34.08	33.15	2.982	0.325	0.076	0.190
球蛋白/(g·L^－1) GLB	45.79^b	48.12^b	55.26^a	61.06^a	8.509	< 0.001	< 0.001	< 0.001
总胆固醇/(mmol·L^－1) T-CHO	2.32	2.63	2.53	2.38	0.308	0.182	0.853	0.113
肌酐/(μmol·L^－1) CRE	147.31	141.41	153.71	148.80	20.904	0.733	0.626	0.888
甘油三酯/(mmol·L^－1) TG	0.56^b	0.67^b	0.62^b	0.88^a	0.217	0.015	0.007	0.016
高密度脂蛋白/(mmol·L^－1) HDL	0.41	0.35	0.30	0.37	0.111	0.241	0.303	0.150
低密度脂蛋白/(mmol·L^－1) LDL	1.71^ab	1.86^a	1.60^b	1.45^b	0.271	0.015	0.015	0.013
生长激素/(ng·mL^－1) GH	2.85	3.04	2.66	2.32	0.619	0.119	0.046	0.064
三碘甲状腺原氨酸/(ng·mL^－1) T₃	6.85	7.47	8.00	8.67	1.694	0.183	0.025	0.085
甲状腺素/(ng·mL^－1) T₄	65.59	67.54	74.41	81.24	13.774	0.095	0.012	0.040
肾上腺素/(ng·L^－1) EPI	498.02	508.75	563.14	675.09	157.184	0.096	0.018	0.040
胰岛素/(mIU·L^－1) INS	17.53	18.95	19.48	23.34	4.850	0.101	0.018	0.050
胰高血糖素/(ng·L^－1) GC	185.45	181.85	173.00	146.90	35.369	0.127	0.026	0.056
皮质酮/(ng·mL^－1) CORT	102.45	106.16	127.67	136.58	29.513	0.054	0.007	0.026
谷胱甘肽还原酶/(U·L^－1) GR	12.06	11.86	12.26	9.85	2.169	0.097	0.073	0.069
胰岛素样生长因子1/(ng·mL^－1) IGF-1	85.06	89.28	110.45	115.49	29.337	0.096	0.014	0.053
胰岛素样生长因子2/(ng·mL^－1) IGF-Ⅱ	19.72	19.50	19.65	21.34	2.770	0.537	0.266	0.342

Table 5

Table 6

References 57

1	FORTE C , MOSCATI L , ACUTI G , et al. Effects of dietary Lactobacillus acidophilus and Bacillus subtilis on laying performance, egg quality, blood biochemistry and immune response of organic laying hens[J]. J Anim Physiol Anim Nutr (Berl), 2016, 100 (5): 977- 987. doi: 10.1111/jpn.12408
2	FREEDMAN K E , HILL J L , WEI Y R , et al. Examining the gastrointestinal and immunomodulatory effects of the novel probiotic Bacillus subtilis DE111[J]. Int J Mol Sci, 2021, 22 (5): 2453. doi: 10.3390/ijms22052453
3	乌日勒格. 日粮中添加枯草芽孢杆菌对育肥羊生长性能、血液指标及瘤胃微生物的影响[D]. 呼和浩特: 内蒙古农业大学, 2019.
	WU R L G. Adding Bacillus subtillis to the diet to improve the growth performance of the finishing sheep, blood indicators and effects of rumen microbes[D]. Hohhot: Inner Mongolia Agricultural University, 2019. (in Chinese)
4	刘俊斌, 张利平, 宋淑珍. 枯草芽孢杆菌对湖羊胃肠道发育、瘤胃发酵参数及微生物区系的影响[J]. 动物营养学报, 2022, 34 (9): 5984- 5994.
	LIU J B , ZHANG L P , SONG S Z . Effects of Bacillus subtilis on gastrointestinal development, rumen fermentation parameters and microflora of Hu sheep[J]. Chinese Journal of Animal Nutrition, 2022, 34 (9): 5984- 5994.
5	何振虎, 项黎丽. 枯草芽孢杆菌对育肥羊生长性能、抗氧化及免疫机能的影响[J]. 中国饲料, 2023, (16): 21- 24.
	HE Z H , XIANG L L . Effects of Bacillus subtilis on growth performance, antioxidant and immune function of fattening sheep[J]. China Feed, 2023, (16): 21- 24.
6	刘俊斌, 宋淑珍, 张利平. 枯草芽孢杆菌对断奶湖羊生长性能、屠宰性能和肉品质的影响[J]. 动物营养学报, 2022, 34 (5): 3096- 3106. doi: 10.3969/j.issn.1006-267x.2022.05.037
	LIU J B , SONG S Z , ZHANG L P . Effects of Bacillus subtilis on growth performance, slaughter performance and meat quality of weaned Hu sheep[J]. Chinese Journal of Animal Nutrition, 2022, 34 (5): 3096- 3106. doi: 10.3969/j.issn.1006-267x.2022.05.037
7	韩林. 木聚糖酶和枯草芽孢杆菌对牛生长性能和瘤胃发酵的影响[D]. 太原: 山西农业大学, 2021.
	HAN L. Effects of xylanase and Bacillus subtilis on growth performance and rumen fermentation in Holstein dairy bulls[D]. Taiyuan: Shanxi Agricultural University, 2021. (in Chinese)
8	李秀丽, 丁应龙, 孟宪国. 不同水平的酿酒酵母复合物对肉牛增重的影响[J]. 饲料研究, 2021, 44 (9): 25- 28.
	LI X L , DING Y L , MENG X G . Effect of different levels of Saccharomyces cerevisiae complex on weight gain of beef cattle[J]. Feed Research, 2021, 44 (9): 25- 28.
9	李靖. 枯草芽孢杆菌对荷斯坦育成牛生长性能和瘤胃微生物区系的影响[D]. 泰安: 山东农业大学, 2020.
	LI J. Effects of Bacillus subtilis on growth performance and rumen microflora in Holstein cows[D]. Tai'an: Shandong Agricultural University, 2020. (in Chinese)
10	VAN SOEST P J , ROBERTSON J B , LEWIS B A . Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J]. J Dairy Sci, 1991, 74 (10): 3583- 3597. doi: 10.3168/jds.S0022-0302(91)78551-2
11	冯宗慈, 高民. 通过比色测定瘤胃液氨氮含量方法的改进[J]. 畜牧与饲料科学, 2010, 31 (6-7): 37.
	FENG Z C , GAO M . Improvement of colorimetric determination of ammonia nitrogen content in rumen fluid[J]. Animal Husbandry and Feed Science, 2010, 31 (6-7): 37.
12	CONE J W , VAN GELDER A H , DRIEHUIS F . Description of gas production profiles with a three-phasic model[J]. Anim Feed Sci Technol, 1997, 66 (1-4): 31- 45. doi: 10.1016/S0377-8401(96)01147-9
13	曹庆云, 周武艺, 朱贵钊, 等. 气相色谱测定羊瘤胃液中挥发性脂肪酸方法研究[J]. 中国饲料, 2006, (24): 26- 28.
	CAO Q Y , ZHOU W Y , ZHU G Z , et al. Study on the methods of determination of volatile fatty acid in the rumen liquid of lambs by gas chromatography[J]. China Feed, 2006, (24): 26- 28.
14	FEEDAP , BAMPIDIS V , AZIMONTI G , et al. Efficacy of Bacillus subtilis DSM 28343 as a zootechnical additive (gut flora stabiliser) for calves for rearing[J]. EFSA J, 2019, 17 (7): e05793.
15	仇武松, 王彦芦, 张振威, 等. 日粮添加产朊假丝酵母与枯草芽孢杆菌对湖羊生长性能及养分消化率的影响[J]. 中国畜牧杂志, 2017, 53 (2): 106- 109.
	QIU W S , WANG Y L , ZHANG Z W , et al. Effects of dietary candida utilis and Bacillus subtilis addition on growth performance and nutrient digestibility of Hu sheep[J]. Chinese Journal of Animal Science, 2017, 53 (2): 106- 109.
16	申远航, 聂海涛, 张艳丽, 等. 益生菌发酵饲料在反刍动物生产上的应用[J]. 家畜生态学报, 2018, 39 (7): 6- 10.
	SHEN Y H , NIE H T , ZHANG Y L , et al. Application of probiotic fermentation feed in ruminant production[J]. Journal of Domestic Animal Ecology, 2018, 39 (7): 6- 10.
17	DUMITRU M , SORESCU I , HABEANU M , et al. Preliminary characterisation of Bacillus subtilis strain use as a dietary probiotic bio-additive in weaning piglet[J]. Food Feed Res, 2018, 45 (2): 203- 211.
18	AKHTAR N , CAI H Y , KIARIE E G , et al. A novel Bacillus sp.with rapid growth property and high enzyme activity that allows efficient fermentation of soybean meal for improving digestibility in growing pigs[J]. J Appl Microbiol, 2022, 133 (1): 3- 17.
19	LI H H , JIANG X R , QIAO J Y . Effect of dietary Bacillus subtilis on growth performance and serum biochemical and immune indexes in weaned piglets[J]. J Appl Anim Res, 2021, 49 (1): 83- 88.
20	张娟利, 莫放, 姚刚, 等. 日粮添加枯草芽孢杆菌对13月龄新疆褐牛育肥增重性能和消化率的影响[J]. 中国畜牧杂志, 2020, 56 (12): 153- 157.
	ZHANG J L , MO F , YAO G , et al. Effect of dietary Bacillus subtilis supplementation on feedlotting performance and nutrient digestibility of Xinjiang brown beef cattle at initial age of thirteen month[J]. Chinese Journal of Animal Science, 2020, 56 (12): 153- 157.
21	白天天, 崔浩然, 赵林波, 等. 添加屎肠球菌、枯草芽孢杆菌及其复合菌对绵羊生长性能、养分表观消化率、瘤胃发酵指标和瘤胃微生物区系的影响[J]. 动物营养学报, 2022, 34 (8): 5190- 5205.
	BAI T T , CUI H R , ZHAO L B , et al. Effects of adding Enterococcus faecalis, Bacillus subtilis and their compound bacteria on growth performance, nutrient apparent digestibility, rumen fermentation Indexes and rumen microflora of sheep[J]. Chinese Journal of Animal Nutrition, 2022, 34 (8): 5190- 5205.
22	田跃, 董红刚, 沈泽锋. 饲用枯草芽孢杆菌对育肥猪生长性能、养分消化、粪便特征及经济效益的影响[J]. 中国饲料, 2021, 1 (8): 45- 48.
	TIAN Y , DONG H G , SHEN Z F . Effects of feed Bacillus subtilis on growth performance, nutrient digestion, fecal characteristics and economic benefit of finishing pigs[J]. China Feed, 2021, 1 (8): 45- 48.
23	张安荣, 刘娇, 陈志敏, 等. 枯草芽孢杆菌对肉仔鸡排泄物中钙、磷含量及养分表观代谢率的影响[J]. 中国饲料, 2020, (19): 53- 56.
	ZHANG A R , LIU J , CHEN Z M , et al. Effects of dietary Bacillus subtilis on calcium and phosphorus contents in feces and the apparent metabolic rate of nutrient of broilers[J]. China Feed, 2020, (19): 53- 56.
24	于小杰, 王净, 白园园, 等. 放牧与舍饲饲养方式对小尾寒羊肉品质的影响[J]. 畜牧兽医学报, 2021, 52 (8): 2223- 2232.
	YU X J , WANG J , BAI Y Y , et al. Effects of grazing and confinement feeding systems on the meat quality of small-tailed Han Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52 (8): 2223- 2232.
25	CUI C , SHEN C J , JIA G , et al. Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism[J]. Genet Mol Res, 2013, 12 (2): 1766- 1776.
26	BAI W K , ZHANG F J , HE T J , et al. Dietary probiotic Bacillus subtilis strain fmbj increases antioxidant capacity and oxidative stability of chicken breast meat during storage[J]. PLoS One, 2016, 11 (12): e0167339.
27	MENG Q W , YAN L , AO X , et al. Influence of probiotics in different energy and nutrient density diets on growth performance, nutrient digestibility, meat quality, and blood characteristics in growing-finishing pigs[J]. J Anim Sci, 2010, 88 (10): 3320- 3326.
28	宋淑珍, 王彩莲, 吴建平, 等. 枯草芽孢杆菌和紫锥菊提取物对育肥羊生长性能、免疫性能和肉品质的影响[J]. 动物营养学报, 2018, 30 (3): 1084- 1094.
	SONG S Z , WANG C L , WU J P , et al. Effects of Bacillus subtilis and Echinacea extract on growth performance, immunity and meat quality of fattening sheep[J]. Chinese Journal of Animal Nutrition, 2018, 30 (3): 1084- 1094.
29	WU J , SONG S , WANG C , et al. PSXV-23 The effect of Bacillus subtilis or Ehinacea on growth performance, meat quality and immune indexes in lambs[J]. J Anim Sci, 2018, 96 (Suppl_3): 473- 474.
30	刘帅, 熊云霞, 孙铝辉, 等. 枯草芽孢杆菌在猪生产中的应用及其作用机制的研究进展[J]. 动物营养学报, 2022, 34 (4): 2105- 2113.
	LIU S , XIONG Y X , SUN L H , et al. Research progress of application of Bacillus subtilis as feed additive in pigs production[J]. Chinese Journal of Animal Nutrition, 2022, 34 (4): 2105- 2113.
31	郭瑞萍, 肖发沂, 都振玉, 等. 枯草芽孢杆菌对肉鸡生产性能、屠宰性能、免疫性能和肌肉风味的影响[J]. 中国饲料, 2019, (17): 79- 83.
	GUO R P , XIAO F Y , DU Z Y , et al. Effects of Bacillus subtilis on the production performance, slaughter performance, immune performance and muscle flavor of broiler chickens[J]. China Feed, 2019, (17): 79- 83.
32	彭豫东, 康克浪, 曲湘勇, 等. 枯草芽孢杆菌对石门土鸡生长性能、屠宰性能、血清抗氧化指标和肠道形态的影响[J]. 动物营养学报, 2019, 31 (5): 2119- 2126.
	PENG Y D , KANG K L , QU X Y , et al. Effects of Bacillus subtilis on growth performance, slaughter performance, serum antioxidant indexes and intestinal morphology of Shimen chickens[J]. Chinese Journal of Animal Nutrition, 2019, 31 (5): 2119- 2126.
33	李洪涛, 杨在宾, 杨维仁, 等. 不同日粮类型对杜泊羊瘤胃pH、氨态氮和挥发性脂肪酸浓度的影响[J]. 中国畜牧杂志, 2017, 53 (3): 67- 72.
	LI H T , YANG Z B , YANG W R , et al. Effects of different diet types on Ruminal pH, NH₃-N and VFA concentration of Dorper sheep[J]. Chinese Journal of Animal Science, 2017, 53 (3): 67- 72.
34	李宏, 宋淑珍, 高良霜, 等. 饲养水平对阿勒泰羊胃肠道发育、瘤胃发酵参数及瘤胃微生物区系的影响[J]. 草业学报, 2021, 30 (4): 180- 190.
	LI H , SONG S Z , GAO L S , et al. Effects of feeding level on the gastrointestinal development, rumen fermentation and rumen microbiota in Altay sheep[J]. Acta Prataculturae Sinica, 2021, 30 (4): 180- 190.
35	BANNINK A , FRANCE J , LOPEZ S , et al. Modelling the implications of feeding strategy on rumen fermentation and functioning of the rumen wall[J]. Anim Feed Sci Technol, 2008, 143 (1-4): 3- 26.
36	李月明, 栾嘉明, 张敏, 等. 不同浓度枯草芽孢杆菌对体外瘤胃发酵特性的影响[J]. 中国畜牧兽医, 2019, 46 (4): 1031- 1037.
	LI Y M , LUAN J M , ZHANG M , et al. Effects of Bacillus subtilis on in vitro rumen fermentation characteristics[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46 (4): 1031- 1037.
37	程连平, 贺濛初, 夏晓冬, 等. 富硒酵母和枯草芽孢杆菌对湖羊羔羊生长性能、血清指标和消化功能的影响[J]. 动物营养学报, 2018, 30 (8): 3189- 3198.
	CHENG L P , HE M C , XIA X D , et al. Effects of Selenium-enriched yeast and Bacillus subtilis on growth performance, serum indices, digestive function of Hu lambs[J]. Chinese Journal of Animal Nutrition, 2018, 30 (8): 3189- 3198.
38	SUN P , WANG J Q , DENG L F . Effects of Bacillus subtilis natto on milk production, rumen fermentation and ruminal microbiome of dairy cows[J]. Animal, 2013, 7 (2): 216- 222.
39	PENG H , WANG J Q , KANG H Y , et al. Effect of feeding Bacillus subtilis natto fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows[J]. J Anim Physiol Anim Nutr (Berl), 2012, 96 (3): 506- 512.
40	KALMENDAL R , TAUSON R . Effects of a xylanase and protease, individually or in combination, and an ionophore coccidiostat on performance, nutrient utilization, and intestinal morphology in broiler chickens fed a wheat-soybean meal-based diet[J]. Poult Sci, 2012, 91 (6): 1387- 1393.
41	王喜峰, 魏霖, 贺绍君, 等. 枯草芽孢杆菌和乳酸菌对热应激肉鸡生长性能和肝肾功能的影响[J]. 安徽科技学院学报, 2019, 33 (6): 23- 28.
	WANG X F , WEI L , HE S J , et al. Effects of Bacillus subtilis and lactobacillus on growth performance and liver and kidney function of broilers under heat stress[J]. Journal of Anhui Science and Technology University, 2019, 33 (6): 23- 28.
42	王毅, 田斌, 陈亮, 等. 枯草芽孢杆菌在湖羊羔羊强制补饲料中应用效果研究[J]. 中国草食动物科学, 2022, 42 (2): 61- 64.
	WANG Y , TIAN B , CHEN L , et al. Research on the application effect of Bacillus subtilis in compulsory feed for Hu sheep lambs[J]. China Herbivore Science, 2022, 42 (2): 61- 64.
43	苏勇华, 方雷, 应璐, 等. 枯草芽孢杆菌对多浪羊消化率、瘤胃发酵参数及血液指标的影响[J]. 江苏农业科学, 2018, 46 (8): 162- 166.
	SU Y H , FANG L , YING L , et al. Effects of Bacillus subtilis on nutrient digestibility, rumen fermentation parameters and blood indices of Duolang sheep[J]. Jiangsu Agricultural Sciences, 2018, 46 (8): 162- 166.
44	SOLTANZADEH S , ESMAEILI FEREIDOUNI A , OURAJI H , et al. Growth performance, body composition, hematological, and serum biochemical responses of beluga (Huso huso) juveniles to different dietary inclusion levels of faba bean (Vicia faba) meal[J]. Aquacult Int, 2016, 24 (1): 395- 413.
45	张燕. 枯草芽孢杆菌对肉鸡生产性能、血清代谢产物和肠道健康的影响[D]. 泰安: 山东农业大学, 2023.
	ZHANG Y. Effects of Bacillus subtilis on the growth performance, serum metabolites and intestinal health in broiler chickens[D]. Tai'an: Shandong Agricultural University, 2023. (in Chinese)
46	蒋小丰, 方热军. 丁酸在动物体内的作用[J]. 饲料工业, 2008, 29 (20): 51- 54.
	JIANG X F , FANG R J . Action of butyric acid in animal body[J]. Feed Industry, 2008, 29 (20): 51- 54.
47	张安荣, 刘娇, 陈志敏, 等. 饲粮添加枯草芽孢杆菌对肉仔鸡生长性能、血清生化指标和胫骨指标的影响[J]. 动物营养学报, 2020, 32 (9): 4123- 4131.
	ZHANG A R , LIU J , CHEN Z M , et al. Effect of dietary Bacillus subtilis on growth performance, serum biochemical indices and tibia Index of broilers[J]. Chinese Journal of Animal Nutrition, 2020, 32 (9): 4123- 4131.
48	张洪伟, 周英昊, 张磊, 等. 酵母培养物、枯草芽孢杆菌对育肥牛生产性能、生化指标、肠道菌群及经济效益的影响[J]. 饲料研究, 2022, 45 (18): 10- 13.
	ZHANG H W , ZHOU Y H , ZHANG L , et al. Effects of yeast culture and Bacillus subtilis on production performance, biochemical indexes, intestinal flora and economic benefits of fattening cattle[J]. Feed Research, 2022, 45 (18): 10- 13.
49	丁浩, 黄攀, 章文明, 等. 饲粮添加枯草芽孢杆菌对保育猪生长性能和血浆生化参数的影响[J]. 动物营养学报, 2020, 32 (2): 605- 612.
	DING H , HUANG P , ZHANG W M , et al. Effects of dietary Bacillus subtilis on growth performance and plasma biochemical parameters of nursery piglets[J]. Chinese Journal of Animal Nutrition, 2020, 32 (2): 605- 612.
50	宋林锦. 关岭牛IGF1、MSTN对成肌细胞增殖的作用研究[D]. 贵阳: 贵州大学, 2023.
	SONG L J. Study on the effects of IGF1 and MSTN on the proliferation of myogenic cells in Guanling bovine[D]. Guiyang: Guizhou University, 2023. (in Chinese)
51	李泽然. 枯草芽孢杆菌对肉鸡生长性能、屠宰性能、肉品质、免疫功能、抗氧化功能和肠道形态的影响[J]. 饲料研究, 2023, 46 (11): 51- 55.
	LI Z R . Effect of Bacillus subtilis on growth performance, slaughter performance, meat quality, immune function, antioxidant function and intestinal morphology of broilers[J]. Feed Research, 2023, 46 (11): 51- 55.
52	SINGH S , SHARMA R K , MALHOTRA S , et al. Lactobacillus rhamnosus NCDC17 ameliorates type-2 diabetes by improving gut function, oxidative stress and inflammation in high-fat-diet fed and streptozotocintreated rats[J]. Benefic Microbes, 2017, 8 (2): 243- 256.
53	王蕾, 孙娅静. 枯草芽孢杆菌对AA肉仔鸡血清抗氧化功能和肉品质的影响[J]. 饲料研究, 2020, 43 (9): 36- 39.
	WANG L , SUN Y J . Effect of Bacillus subtilis on serum antioxidant function and meat quality of AA broilers[J]. Feed Research, 2020, 43 (9): 36- 39.
54	黄嘉訸. 日粮添加枯草芽孢杆菌对生长育肥猪生长性能和肉品质的影响[D]. 杨凌: 西北农林科技大学, 2023.
	HUANG J H. Effect of dietary Bacillus subtilis supplementation on growth performance and meat quality of growing-finishing pigs[D]. Yangling: Northwest A & F University, 2023. (in Chinese)
55	韩鹏敏, 张然, 边世雄, 等. 屎肠球菌和枯草芽孢杆菌对乳鸽生长性能、屠宰性能和免疫功能的影响[J]. 畜牧兽医学报, 2022, 53 (11): 3880- 3891.
	HAN P M , ZHANG R , BIAN S X , et al. Effects of Enterococcus faeciumand and Bacillus subtilis on growth performance, carcass traits and immune function of squabs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53 (11): 3880- 3891.
56	袁建彬, 郑树森, 杨焕民, 等. 急性期蛋白作为动物应激诊断标志物的研究进展[J]. 中国生物制品学杂志, 2018, 31 (10): 1156- 1159.
	YUAN J B , ZHENG S S , YANG H M , et al. Advances in study of acute phase protein as a marker for diagnosis of animal stress[J]. Chinese Journal of Biologicals, 2018, 31 (10): 1156- 1159.
57	CECILIANI F , CERON J J , ECKERSALL P D , et al. Acute phase proteins in ruminants[J]. J Proteomics, 2012, 75 (14): 4207- 4231.

项目 Item	含量/% Content
饲粮组成 Ingredient
白酒糟 Bistillers’ grains	34.39
啤酒糟 Brewer’s spent grains	10.28
小麦秸秆 Wheat stover	3.85
青贮皇竹草 Pennisetum hydridum silage	2.93
玉米 Corn	29.15
豆粕 Soybean meal	10.08
棉籽粕 Cottonseed meal	4.95
小苏打 NaHCO₃	0.95
食盐 NaCl	0.50
预混料¹ Premix	2.65
合计 Total	100
营养成分² Nutrient composition
干物质 DM	92.50
有机物 OM	90.47
粗蛋白 CP	13.62
粗脂肪 EE	4.66
中性洗涤纤维 NDF	33.44
酸性洗涤纤维 ADF	19.03
钙 Ca	0.69
磷 P	0.29

项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	P值 P-value
项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	Treat	L	Q
初重/kg Initial weight	561.21	558.18	557.20	552.49	23.429	0.973	0.633	0.894
末重/kg Final weight	648.00	661.33	658.50	668.50	28.125	0.829	0.385	0.691
干物质采食量/(kg·d^－1) DMI	10.61	11.08	10.74	10.54	0.586	0.646	0.939	0.573
平均日增重/(kg·d^－1) ADG	0.97^b	1.15^ab	1.13^ab	1.29^a	0.169	0.049	0.005	0.024
料重比F/G	11.10^a	9.74^ab	9.73^ab	8.19^b	1.759	0.015	0.002	0.010
胸深/cm Chest depth	73.33	72.50	73.67	74.17	3.947	0.921	0.629	0.827
体斜长/cm Body length	151.83	153.67	152.67	152.67	10.853	0.995	0.943	0.979
胸围/cm Chest girth	217.33	208.00	208.17	209.33	9.467	0.293	0.183	0.168
尻宽/cm Hip width	25.83	26.33	27.67	26.33	2.000	0.471	0.460	0.428
尻长/cm Hip length	23.17	23.50	26.67	24.67	2.582	0.080	0.113	0.158
干物质/% DM	70.71	69.47	69.96	69.54	0.914	0.514	0.295	0.478
有机物/% OM	74.72	73.64	74.90	73.44	1.465	0.679	0.577	0.844
粗脂肪/% EE	71.56	71.77	74.12	70.80	2.608	0.820	0.991	0.635
粗蛋白/% CP	66.08	64.34	65.31	64.28	2.206	0.617	0.518	0.793
中性洗涤纤维/% NDF	56.33	56.82	58.38	55.38	2.447	0.668	0.865	0.593
酸性洗涤纤维/% ADF	53.84	49.74	53.61	51.51	2.524	0.339	0.703	0.806
钙/% Ca	47.57	40.33	46.64	42.28	4.307	0.236	0.498	0.720
磷/% P	55.29	50.79	54.60	53.02	2.305	0.296	0.695	0.645

项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	P值 P-value
项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	Treat	L	Q
胴体重/kg Carcass weight	378.87	376.53	385.87	400.73	33.290	0.633	0.236	0.417
屠宰率/% Dressing percentage	53.00	53.61	55.00	55.09	2.060	0.305	0.043	0.129
眼肌面积/cm² Eye muscle area	143.70	141.66	141.46	151.35	20.348	0.845	0.561	0.672
背膘厚/mm Backfat thickness	11.34	10.23	10.73	12.66	2.382	0.358	0.324	0.192
西冷部位重量/kg Weight of sirloin	2.935	2.990	2.957	3.019	0.397	0.988	0.776	0.961
剪切力/N Shear force	37.05	43.11	34.60	40.49	5.374	0.680	0.940	0.997
蒸煮损失/% Cooking loss	26.54	27.82	25.49	27.61	7.332	0.965	0.957	0.998
滴水损失/% Drip loss	1.37	1.75	0.86	1.80	1.371	0.673	0.930	0.909
45 min pH	6.49	6.36	6.60	6.57	0.321	0.619	0.436	0.694
45 min亮度 L_{45 min}^*	18.86	21.04	20.08	19.91	3.468	0.790	0.345	0.634
45 min红度 a_{45 min}^*	9.84	11.49	11.47	10.41	2.295	0.563	0.702	0.354
45 min黄度 b_{45 min}^*	2.81	3.00	2.87	3.06	0.757	0.948	0.675	0.918
水分/% Moisture	75.79	75.84	76.33	75.89	1.100	0.856	0.684	0.823
粗脂肪/% EE	1.64	2.09	1.38	2.08	0.074	0.312	0.745	0.901
粗蛋白/% CP	19.71	20.19	19.59	19.49	0.060	0.259	0.323	0.336
粗灰分/% Ash	0.99	0.97	0.99	0.93	0.051	0.303	0.164	0.281

项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	P值 P-value
项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	Treat	L	Q
pH	6.30	6.99	6.82	6.61	0.513	0.111	0.483	0.072
氨态氮/(mg·dL^－1) NH₃-N	12.92	13.08	12.83	13.07	0.220	0.169	0.672	0.839
微生物蛋白/(mg·dL^－1) MCP	141.59	164.48	124.12	147.15	18.62	0.222	0.707	0.933
总挥发性脂肪酸/(mmol·L^－1) Total VFA	38.96	23.70	21.20	30.69	13.743	0.114	0.296	0.047
乙酸/% Acetate	58.14	60.60	62.73	61.96	5.796	0.584	0.216	0.380
丙酸/% Propionate	21.83	21.49	20.21	19.84	5.890	0.937	0.523	0.819
丁酸/% Butyrate	14.75^a	10.58^b	9.61^b	12.17^ab	2.931	0.008	0.113	0.002
异丁酸/% Isobutyrate	1.89	2.80	2.74	2.17	0.971	0.110	0.687	0.178
异戊酸/% Isovalerate	2.57	3.60	3.95	2.96	0.289	0.329	0.454	0.048
戊酸/% Valerate	0.82	0.93	0.76	0.90	1.065	0.759	0.915	0.991
乙酸/丙酸 Acetate: Propionate	4.28^b	5.87^a	6.56^a	5.27^ab	1.412	0.031	0.170	0.012

项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	P值 P-value
项目 Item	对照组 CON	低剂量组 LAL	中剂量组 LAM	高剂量组 LAH	标准误 SEM	Treat	L	Q
总抗氧化能力/(mmol·L^－1) T-AOC	0.12^b	0.17^a	0.12^b	0.10^b	0.035	< 0.001	0.084	0.007
丙二醛/(nmol·mL^－1) MDA	7.35	7.29	6.63	8.05	1.514	0.552	0.640	0.503
超氧化物歧化酶/(U·mL^－1) SOD	206.05	204.28	208.72	208.52	26.119	0.986	0.783	0.960
免疫球蛋白A/(mg·mL^－1) IgA	2.72	2.62	3.09	3.15	0.691	0.337	0.109	0.268
免疫球蛋白G/(mg·mL^－1) IgG	7.83	8.23	9.24	9.41	1.438	0.078	0.010	0.039
脂多糖结合蛋白/(μmol·mL^－1) LBP	12.95	13.67	16.75	14.89	3.236	0.099	0.087	0.124
肿瘤坏死因子-α/(ng·L^－1) TNF-α	95.55	99.69	100.87	112.46	15.442	0.161	0.034	0.086
白细胞介素-1β/(ng·L^－1) IL-1β	20.60	20.94	21.51	23.25	2.961	0.307	0.073	0.163
白细胞介素-2/(ng·L^－1) IL-2	44.30	46.23	54.89	56.05	11.535	0.095	0.015	0.055
白细胞介素-6/(ng·L^－1) IL-6	88.24	90.87	94.38	102.98	14.481	0.208	0.038	0.101
白细胞介素-8/(ng·L^－1) IL-8	94.60	98.19	102.48	110.73	15.307	0.189	0.030	0.089
结合珠蛋白/(μg·mL^－1) Hp	265.40^b	291.25^ab	333.97^ab	378.12^a	87.225	0.049	0.005	0.018
干扰素-γ/(ng·L^－1) IFN-γ	71.46	75.15	76.46	82.22	16.106	0.639	0.199	0.438
淀粉样蛋白/(μg·mL^－1) ASAA	32.77^b	32.71^b	40.74^ab	47.04^a	9.601	0.003	0.001	0.001

Effects of Bacillus Subtilis Preparations on Performance, Ruminal Fermentation, Blood Biochemical and Immune Indices of Beef Cattle

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