CD163基因敲除猪对胸膜肺炎放线杆菌、猪链球菌和副猪嗜血杆菌的易感性研究

doi:10.11843/j.issn.0366-6964.2024.12.014

Abstract

Abstract:

This study aimed to investigate whether pigs with a CD163 gene knockout (CD163-KO) resulting from a single base insertion in the SRCR5 domain of the CD163 gene exhibit altered susceptibility to 3 crucial bacterial pathogens compared to their wild-type (WT) counterparts and to evaluate the biosafety profile of CD163-KO pigs. This study selected a total of 34 Large White pigs, 17 CD163-KO pigs and 17 wild-type pigs, all at the age of 50 days with similar body weights and health statuses. Among them, 2 CD163-KO Large White pigs and 2 WT Large White pigs were used as blank controls without any infection treatment. None of the pigs had a history of infection or vaccination against Actinobacillus pleuropneumoniae (APP), Streptococcus suis (SS), and Haemophilus parasuis (HPS). The pigs were randomly divided into 3 groups of 5 CD163-KO and 5 WT pigs each: group A received APP serotype 1, group B received SS serotype 2, and group C received HPS serotype 13. The pigs were observed continuously for 8 days, the clinical symptoms and mortality were recorded. Upon death, the pigs were promptly necropsied to observe lesions in critical organs such as the heart, thorax, and lungs. Lung samples were collected, and bacterial DNA was extracted from the lungs for quantitative PCR analysis. The relative DNA copy numbers of the 3 bacterial species in the lungs of deceased CD163-KO and WT pigs were statistically analyzed and compared. The primary aim of this study was to evaluate whether CD163-KO pigs exhibit altered susceptibility to these 3 key bacterial pathogens compared to their WT counterparts, ultimately assessing the biosafety profile of CD163-KO pigs. Based on the clinical manifestations observed in the experimental pigs, all groups successfully contracted the respective bacterial infections, and there were no significant differences in survival rates between CD163-KO and WT pigs within each group (P>0.05). Furthermore, according to the pathological findings in the lungs of deceased pigs and the results of quantitative PCR analysis for bacterial DNA, no significant differences were detected in the relative DNA copy numbers of APP serotype 1, SS serotype 2, and HPS serotype 13 strains in the lungs between deceased CD163-KO and WT pigs (P>0.05). The findings of this study serve as robust evidence for the biological safety of CD163-KO Large White pigs, demonstrating that the knockout of the CD163 gene does not enhance the susceptibility of pigs to APP, SS, and HPS, nor does it significantly compromise their ability to resist these bacterial infections, which provides strong support for the biological safety assessment of CD163-KO pigs.

Key words: CD163, gene knockout, Large White pig, porcine reproductive and respiratory syndrome virus, Actinobacillus pleuropneumoniae, Streptococcus suis, Haemophilus parasuis, biosafety assessment

CLC Number:

S828.2

LIANG Guohao, HU Dandan, ZHONG Haiwen, ZHANG Jian, YANG Dehong, WU Zhenfang, YANG Huaqiang, ZHANG Xianwei. Study on the Susceptibility of CD163 Gene Knockout Pigs to Actinobacillus pleuropneumoniae, Streptococcus suis, and Haemophilus parasuis[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5478-5488.

Figures/Tables 7

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References 33

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组别 Group		数量 Number	菌株 Strain	注射量/(mL·头^-1) Injection volume	菌液浓度/(cfu·mL^-1) Concentration of bacterium	攻毒方式 Attack mode
A	A1	5头CD163-KO型	APP101	2	2.2×10⁸^[24]	气管注射
	A2	5头WT型
B	B1	5头CD163-KO型	SS201	2	8.0×10⁷^[25]	腹腔注射
	B2	5头WT型
C	C1	5头CD163-KO型	HPS1301	2	3.6×10⁹^[26]	腹腔注射
	C2	5头WT型

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Study on the Susceptibility of CD163 Gene Knockout Pigs to Actinobacillus pleuropneumoniae, Streptococcus suis, and Haemophilus parasuis

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