β-烟酰胺单核苷酸对牛卵母细胞脂滴含量及冷冻效果的影响

doi:10.11843/j.issn.0366-6964.2023.08.020

Abstract

Abstract: The aim of this study was to investigate the effect of β-nicotinamide mononucleotide (NMN) on the lipid droplet content and vitrification freezing of bovine oocytes. In this study, bovine oocytes were placed in medium containing 1 μmol·L^-1NMN for in vitro maturation (IVM), vitrification and in vitro fertilization (IVF). The lipid droplet content, reactive oxygen species (ROS) level, apoptosis level and developmental ability of bovine oocytes were examined. The group with the addition of NMN was used as a NMN group; The group without the addition of NMN was used as a fresh control group; The group with the addition of NMN and vitrification was used as a NMN vitrification group; The group without the addition of NMN but with vitrification was used as a vitrification control group. The lipid droplet content, ROS level, and apoptosis level in bovine oocytes of the NMN group were significantly lower than those in the fresh control group (P<0.05), and the cleavage rate ((89.57±7.58)%) and blastocyst rate ((45.63±3.78)%) were significantly higher than those of the fresh control group ((80.77±0.70)%, (36.90±1.20)%) (P<0.05). Meanwhile, the ROS level and apoptosis level of bovine oocytes in the NMN vitrification group were significantly lower than those in the vitrification control group (P<0.05), and the survival rate ((97.25±0.11)%), cleavage rate ((75.47±1.11)%) and blastocyst rate ((33.75±1.43)%) after thawing were significantly higher than those in the vitrification control group ((89.29±1.16)%, (52.00±1.26)%, (15.38±1.73)%, P<0.05). In conclusion, the addition of NMN could effectively reduce the lipid droplet content of bovine oocytes and decrease the ROS level and apoptosis level of vitrified bovine oocytes, thus improving the developmental ability of vitrified bovine oocytes.

Key words: NMN, oocyte, lipid droplet, vitrification, ROS, apoptosis

CLC Number:

S823.3

XU Xi, YANG Baigao, ZHANG Hang, FENG Xiaoyi, HAO Haisheng, DU Weihua, ZHU Huabin, ZHANG Peipei, ZHAO Xueming. Effects of NMN on Lipid Droplet Content and Cryopreservation Effect of Bovine Oocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3348-3357.

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Effects of NMN on Lipid Droplet Content and Cryopreservation Effect of Bovine Oocytes

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