基于UPLC-Q-TOF-MS/MS代谢组学研究土霉素和穿心莲内酯对鸡代谢的影响

doi:10.11843/j.issn.0366-6964.2023.07.038

Abstract

Abstract: This experiment was conducted to establish a technical platform for metabolite analysis based on chromatography-mass spectrometry (UPLC-Q-TOF-MS/MS), three-week-old chickens were feed with equal dose of the Chinese veterinary drug andrographolide (AG) and the broad-spectrum antibiotic oxytetracycline (OTC) respectively, to analyze the metabolite changes in the excretion of chickens after intervention. Through statistical analysis of the data, differential metabolites were obtained, and the intervention mechanism of AG and OTC on chicken gut microbiota metabolism were also explored. The excretory samples of yellow-finned chickens in each group were detected by UPLC-Q-TOF-MS/MS, and the metabolic pathways were enriched and analyzed by MetaboAnalyst platform. The differential metabolites were screened by using QI software. The results showed that compared with the blank group, 12 and 10 differential compounds were found in OTC and AG group, respectively. Fifteen metabolic pathways were involved in the OTC group, of which 2 most relevant metabolic pathways were linoleic acid and glycerophospholipid metabolism; 17 metabolic pathways were involved in the AG group, of which 5 most relevant metabolic pathways were glycerophospholipid metabolism, sphingolipid metabolism, cysteine and methionine metabolism, unsaturated fatty acid biosynthesis and glycine, serine and threonine metabolic pathways. It can be concluded that the mechanism of action of OTC and AG affecting chicken metabolism is very similar, both can affect the normal metabolic function of chickens through changing phospholipid metabolism, fatty acid metabolism and amino acid metabolism, especially the distribution pattern of OTC group is significantly different from the control group.

Key words: metabolomics, oxytetracycline, andrographolide, UPLC-Q-TOF-MS/MS

CLC Number:

S859.7

WANG Ruijie, HONG Zhikai, DONG Yingjiao, CHEN Yao, WANG Jinyu, WANG Guanhua. Effect of Oxytetracycline and Andrographolide on the Metabolism of Chicken Intestinal Tracts Using UPLC-Q-TOF-MS/MS-based Metabolomic Approach[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3078-3090.

References 29

[1]	张黎. 叶酸对鸡肝细胞脂肪代谢的作用[J]. 畜牧兽医科学, 2020(17):177-178.ZHANG L. Effects of folic acid on lipid metabolism in chicken liver cells[J]. Graziery Veterinary Sciences, 2020(17):177-178. (in Chinese)
[2]	ХОМЕНКО Н Р, 谢敏康. 呋喃唑酮对鸡氨基酸代谢的影响[J]. 国外畜牧学, 1988(3):42-43.ХОМЕНКО Н Р, XIE M K. Effect of furazolidone on amino acid metabolism in chickens[J]. Animal Science Abroad, 1988(3):42-43. (in Chinese)
[3]	VLASOVA A N, TAKANASHI S, MIYAZAKI A, et al. How the gut microbiome regulates host immune responses to viral vaccines[J]. Curr Opin Virol, 2019, 37:16-25.
[4]	PREIDIS G A, HILL C, GUERRANT R L, et al. Probiotics, enteric and diarrheal diseases, and global health[J]. Gastroenterology, 2011, 140(1):8-14.
[5]	胡滨, 陈一资, 辜雪冬. 鸡土霉素急性、蓄积性毒性试验研究[J]. 广东农业科学, 2010, 37(2):140-143.HU B, CHEN Y Z, GU X D. Experiment on acute and accumulative toxicity test of Oxytetracycline in chicken[J]. Guangdong Agricultural Sciences, 2010, 37(2):140-143. (in Chinese)
[6]	曾晓, 张清伦, 龙林. 穿心莲对普定高脚雏鸡生长及胃肠道发育的影响[J]. 湖南农业科学, 2021(11):69-70, 81.ZENG X, ZHANG Q L, LONG L. Effects of Andrographis paniculata on growth and gastrointestinal health of Puding Long-Leg chicken[J]. Hunan Agricultural Sciences, 2021(11):69-70, 81. (in Chinese)
[7]	陈奇. 中药新药药理研究方法的特点与注意事项[J]. 中药新药与临床药理, 1998, 9(2):72-73.CHEN Q. Characteristics and considerations of pharmacological research methods for new Chinese medicines[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 1998, 9(2):72-73. (in Chinese)
[8]	罗和古, 陈家旭. 代谢组学技术与中医证候的研究[J]. 中国中医药信息杂志, 2007, 14(5):3-5.LUO H G, CHEN J X. Metabolomics technology and the study of Chinese medical evidence[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2007, 14(5):3-5. (in Chinese)
[9]	SEARS C L. A dynamic partnership:celebrating our gut flora[J]. Anaerobe, 2005, 11(5):247-251.
[10]	TAYLOR J, KING R D, ALTMANN T, et al. Application of metabolomics to plant genotype discrimination using statistics and machine learning[J]. Bioinformatics, 2002, 18(Suppl 2):S241-S248.
[11]	AON M A, BERNIER M, MITCHELL S J, et al. Untangling determinants of enhanced health and lifespan through a multi-omics approach in mice[J]. Cell Metab, 2020, 32(1):100-116.
[12]	TSUKAHARA T, MATSUDA Y, HANIU H. Lysophospholipid-related diseases and PPARγ signaling pathway[J]. Int J Mol Sci, 2017, 18(12):2730.
[13]	ZHANG X J, CHOI F F K, ZHOU Y, et al. Metabolite profiling of plasma and urine from rats with TNBS-induced acute colitis using UPLC-ESI-QTOF-MS-based metabonomics-a pilot study[J]. FEBS J, 2012, 279(13):2322-2338.
[14]	SPIEGEL S, MERRILL A H JR. Sphingolipid metabolism and cell growth regulation[J]. FASEB J, 1996, 10(12):1388-1397.
[15]	MURASE T, AOKI M, WAKISAKA T, et al. Anti-obesity effect of dietary diacylglycerol in C57BL/6J mice:dietary diacylglycerol stimulates intestinal lipid metabolism[J]. J Lipid Res, 2002, 43(8):1312-1319.
[16]	FAROOQUI A A, HORROCKS L A, FAROOQUI T. Glycerophospholipids in brain:their metabolism, incorporation into membranes, functions, and involvement in neurological disorders[J]. Chem Phys Lipids, 2000, 106(1):1-29.
[17]	MUNOZ-OLAYA J M, MATABOSCH X, BEDIA C, et al. Synthesis and biological activity of a novel inhibitor of dihydroceramide desaturase[J]. ChemMedChem, 2008, 3(6):946-953.
[18]	LI X, BECKER K A, ZHANG Y. Ceramide in redox signaling and cardiovascular diseases[J]. Cell Physiol Biochem, 2010, 26(1):41-48.
[19]	YANG Z D, CAI X Z, XU X X, et al. Urinary metabolomics identified metabolic disturbance associated with polycystic ovary syndrome[J]. Anal Biochem, 2022, 647:114665.
[20]	D'ANGELO G, MOORTHI S, LUBERTO C. Role and function of sphingomyelin biosynthesis in the development of cancer[J]. Adv Cancer Res, 2018, 140:61-96.
[21]	KASUMOV T, LI L, LI M, et al. Ceramide as a mediator of non-alcoholic Fatty liver disease and associated atherosclerosis[J]. PLoS One, 2015, 10(5):e0126910.
[22]	BASSAGANYA-RIERA J, VILADOMIU M, PEDRAGOSA M, et al. Probiotic bacteria produce conjugated linoleic acid locally in the gut that targets macrophage PPAR γ to suppress colitis[J]. PLoS One, 2012, 7(2):e31238.
[23]	夏珺, 郑明月, 李灵杰, 等. 共轭亚油酸改善肥胖糖尿病小鼠的糖脂代谢[J]. 南方医科大学学报, 2019, 39(6):740-746.XIA J, ZHENG M Y, LI L J, et al. Conjugated linoleic acid improves glucose and lipid metabolism in diabetic mice[J]. Journal of Southern Medical University, 2019, 39(6):740-746. (in Chinese)
[24]	SPINDLER S A, CLARK K S, BLACKBURN M L, et al. Occurrence of 13(S)-Hydroxyoctadecadienoic acid in biological samples[J]. Prostaglandins, 1997, 54(6):875-880.
[25]	INOUYE M, MIO T, SUMINO K. Formation of 9-hydroxy linoleic acid as a product of phospholipid peroxidation in diabetic erythrocyte membranes[J]. Biochim Biophys Acta Mol Cell Biol Lipids, 1999, 1438(2):204-212.
[26]	YOSHIDA Y, SAITO Y, HAYAKAWA M, et al. Levels of lipid peroxidation in human plasma and erythrocytes:comparison between fatty acids and cholesterol[J]. Lipids, 2007, 42(5):439-449.
[27]	廖丽姿, 刘磊, 田卫东. PPARγ天然激动剂的研究进展[J]. 现代生物医学进展, 2009, 9(7):1381-1383.LIAO L Z, LIU L, TIAN W D. Research progress in the natural agonists of PPARγ[J]. Progress in Modern Biomedicine, 2009, 9(7):1381-1383. (in Chinese)
[28]	罗新新, 朱水兰, 李冰涛, 等. 葛根芩连汤激活PPARγ上调脂联素和GLUT4表达改善脂肪胰岛素抵抗[J]. 中国中药杂志, 2017, 42(23):4641-4648.LUO X X, ZHU S L, LI B T, et al. Gegen Qinlian decoction activates PPARγ to ameliorate Adipocytic insulin resistance in diabetic SD rats and IR-3T3-L1 adipocytes[J]. China Journal of Chinese Materia Medica, 2017, 42(23):4641-4648. (in Chinese)
[29]	秦慧真, 林思, 邓玲玉, 等. 穿心莲内酯药理作用及机制研究进展[J]. 中国实验方剂学杂志, 2022, 28(6):272-282.QIN H Z, LIN S, DENG L Y, et al. Pharmacological effect and mechanism of andrographolide:a review[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28(6):272-282. (in Chinese)

Effect of Oxytetracycline and Andrographolide on the Metabolism of Chicken Intestinal Tracts Using UPLC-Q-TOF-MS/MS-based Metabolomic Approach

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