奶牛分娩前后瘤胃代谢物变化规律及其代谢通路研究

doi:10.11843/j.issn.0366-6964.2021.011.015

Abstract

Abstract: The aim of this experiment was to clarify the changes of rumen metabolites and their metabolic pathways of dairy cows before and after parturition. Ten healthy Holstein cows with similar body condition and parity were selected to collect fasting rumen fluid before and after parturition. The changes of rumen fermentation products and their metabolic pathways were analyzed by using UPLC-MS/MS metabonomics technology and pathway analysis method with MetaboAnanlyst 5.0. The results showed that the concentration of 57 rumen metabolites in dairy cows changed significantly before and after delivery, of which 34 metabolites were down-regulated and 23 were up-regulated after delivery. And 9 metabolic pathways (taurine and hypotaurine metabolism, arginine and proline metabolism, arginine biosynthesis, D-glutamine and D-glutamate metabolism, alanine, aspartic acid and glutamate metabolism, starch and sucrose metabolism, galactose metabolism, purine metabolism and vitamin B₆ metabolism) consequently underwent strikingly variation. These results indicated that the composition of rumen microflora related to amino acids, sugars, nucleotides and vitamins occured responsive change in dairy cows at 7~10 days before delivery.

Key words: dairy cows parturition, rumen metabolites, metabolomics, pathway analysis, rumen microorganisms

CLC Number:

S823.91
Q936

ZHANG Ruixue, LIU Xin, XU Xiaofeng, ZHANG Bo, TANG Yulin, REN Man, GUO Yansheng. Study on the Changes of Rumen Metabolites and Metabolic Pathways in Dairy Cows before and after Parturition[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3137-3148.

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Study on the Changes of Rumen Metabolites and Metabolic Pathways in Dairy Cows before and after Parturition

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