基于UPLC-MS/MS技术研究濒死状态下新生羔羊血清代谢物变化

doi:10.11843/j.issn.0366-6964.2020.12.030

Abstract

Abstract: This study aimed to explore the metabolic differences in sera of antemortem neonatal goats based on an epidemiological perspective. A case-control design was used to discover the metabolic differences in serum between the dead newborn goats suffered from a disease and healthy goats at the same age by using UPLC-MS/MS. A total of 13 compounds were significantly different between healthy and dead newborn goats. KEGG analysis showed that these differential produced metabolites mainly involved the synthesis and catabolism of amino acids. The differential produced metabolites in serum are important for us to understand the agonal reaction at the metabolic level when neonatal goats suffering from a disease. This study also provides an advanced research basis for the establishment of disease-related biomarkers for neonatal goats which may suffer from a disease.

Key words: neonatal goats, metabolomics, UPLC-MS/MS, diagnosis technology

CLC Number:

S857.31

CHENG Yan, YANG Chao, XU Liwei, GENG Meimei, TAN Zhiliang, HE Zhixiong. Serum Metabolic Analysis of Antemortem Neonatal Goats Using the Approach of UPLC-MS/MS[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(12): 3199-3204.

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Serum Metabolic Analysis of Antemortem Neonatal Goats Using the Approach of UPLC-MS/MS

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