基于16S rRNA测序分析PRRSV感染仔猪肺和肠道中微生物菌群的变化

doi:10.11843/j.issn.0366-6964.2023.04.030

Abstract

Abstract: The purpose of this study was to investigate the effect of porcine reproductive and respiratory syndrome virus infection on the flora in the lungs and intestines of piglets and the histological changes in the lungs and intestines. Fourteen weaned healthy piglets of 35 day old were randomly divided into infection group (n=7) and control group (n=7) after 7 days of adaptive feeding. The piglets in the infection group were inoculated with 2 mL 1×10⁵ TCID₅₀·mL^-1 PRRSV JTS virus solution, and the piglets in the control group were inoculated with 2 mL DMEM. Three piglets in the infection group died on 10, 12, and 19 days, respectively. The surviving piglets in the experimental group and the control group were euthanized on 21 days, and lung, intestinal tissue samples and intestinal contents were collected. Immunohistochemical staining and HE staining were used to observe the histopathological changes of lung and intestine, and high-throughput sequencing based on 16S rRNA Gene was used to analyze the flora structure of piglet lung and intestine. The results showed that the virus was distributed in the lungs and intestines of piglets in the infection group; Compared with the control group, there were significant inflammatory reactions in the lungs and intestines of the infected group. The analysis of microbial composition, structure and diversity showed that the indexes of Chao1 and ACE of piglets in the infection group increased in the lung and decreased in the intestine, while the indexes of Shannon and Simpon increased in the lung and decreased in most intestines. At the phylum level, the proportion of harmful microorganisms such as Proteus increased, while the proportion of beneficial microorganisms such as Firmicutes decreased; In the infection group, the relative proportion of pasteurebacteriaceae, duodenal lactobacilliaceae, ileal Enterobacteriaceae, cecum, colon and rectum decreased significantly, and the proportion of Pseudomonas in lung and duodenum increased significantly; In terms of genus level, the level of beneficial bacteria and lactic acid bacteria in the infection group decreased significantly, the β diversity proves that the clustering effect of small intestine and large intestine is consistent. It can be seen that PRRSV can cause lung lesions and intestinal inflammation in piglets, and affect the composition, abundance and function of lung and intestinal flora.

Key words: porcine reproductive and respiratory syndrome virus, pulmonary microorganisms, intestinal microorganisms, 16S rRNA gene sequencing

CLC Number:

S852.659.6

LU Mengqi, YANG Wengjie, LI Ping, YU Peng, DONG Ling, NIU Xiaoyu, YANG Keli, ZOU Weihua, SONG Hui. Changes of PRRSV Infection on Microorganisms in Lungs and Intestines of Piglets based on 16S rRNA Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1664-1678.

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Changes of PRRSV Infection on Microorganisms in Lungs and Intestines of Piglets based on 16S rRNA Sequencing

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