8株分离自四川、贵州地区猪繁殖与呼吸综合征病毒毒株的全基因组特征分析

doi:10.11843/j.issn.0366-6964.2020.021

Abstract

Abstract: To understand the molecular epidemiology and genetic variation of porcine reproductive and respiratory syndrome virus (PRRSV) in Sichuan and Guizhou provinces, Southwest China in recent years, 8 PRRSV positive samples collected from the pig farms during 2016-2018 were used for viral isolation and full-length genomic sequences amplification, the genomic recombinations of these sequences were analyzed by using RDP4 and Simplot biological softwores. The results showed that the full length of the genome of 8 PRRSV strains were 15 010-15 321 nt, and the homologies among these strains were 80.9%-91.7%. The amino acid (aa) analysis of Nsp2 showed that four strains showed the same 30 aa deletion pattern as HP-PRRSV, and the other four strains showed the same 131 aa deletion pattern as NADC30. Among them, GZgy17 exhibited a novel "1+19+29 aa" deletion pattern. The ORF5 amino acid analysis showed that a new 1 aa deletion at site 33 was emerging in three isolates. Furthermore, recombination analysis showed that the eight strains exhibited six recombination patterns among different lineages:(1) SCnj16:L8+L1; (2) SCxyz17:L1+L5; (3) SCya18:L1+L3; (4) GZgy17:L8+L3; (5) SCcd16 and Cya17:L1+L8+L5; (6) SCcd16 and SCya17:L1+L8+L3. The results indicated that there are not only multiple lineages of PRRSV strains coexisted in Southwest China, but also complex genetic recombination patterns in the genomes of the isolates. The epidemic situation of PRRSV strains with the above complex genome in China deserves great attention.

Key words: porcine reproductive and respiratory syndrome virus, virus isolation and identification, whole genome, deletion, recombination

CLC Number:

S852.659.6

ZHOU Long, KANG Runmin, ZHANG Yi, XIE Bo, YU Jifeng, LI Chun, ZHANG Bin, TANG Cheng, WANG Hongning. Whole Genome Analysis of Eight Porcine Reproductive and Respiratory Syndrome Viruses Isolated in Sichuan and Guizhou Provinces[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1382-1390.

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Whole Genome Analysis of Eight Porcine Reproductive and Respiratory Syndrome Viruses Isolated in Sichuan and Guizhou Provinces

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