非洲猪瘟病毒诱导猪红细胞凋亡并促进猪外周血单核细胞的吞噬作用

doi:10.11843/j.issn.0366-6964.2022.11.020

Abstract

Abstract: The purpose of this study was to investigate the effect of African swine fever virus (ASFV) on porcine red blood cells (RBCs) and its effect on the porcine peripheral blood mononuclear cells (PBMs). Flow cytometry was used to detect the ability of ASFV to infect porcine primary alveolar macrophages (PAMs) and RBCs, and the percentage of ASFV-induced apoptosis of RBCs was detected. Whether induction of RBCs apoptosis affects the phagocytic ability of PBMs. The results showed that ASFV could not invade erythrocytes, but induced apoptosis of RBCs in a time- and dose-dependent manner. Apoptosis of RBCs at 1, 3, 5 and 7 days after inoculation with varying MOI (0.1,1,3) ASFV were as follows:at the MOI of 0.1, the data were 1.27%, 3.23%, 7.39% and 8.56%, respectively; at the MOI of 1, the data were 1.54%, 3.73%, 8.46% and 10.74%, respectively; at the MOI of 3, the data were 2.65%, 5.01%, 12.44% and 18.61%, respectively. At the same time, apoptotic RBCs can increase the number of yellow-green fluorescent microspheres phagocytosed by PBMs. In conclusion, ASFV cannot invade porcine RBCs, but ASFV induces RBCs apoptosis and promotes the phagocytic ability of PBMs in a time- and dose-dependent manner.

Key words: African swine fever virus, porcine red blood cell, apoptosis, phagocytosis

CLC Number:

S852.659.1

YANG Yunlong, FENG Yongzhi, GAO Qi, ZHENG Jiachen, WANG Heng, ZHANG Guihong, GONG Lang. African Swine Fever Virus Induces Porcine Red Blood Cells Apoptosis and Promotes Phagocytosis of Porcine Peripheral Blood Mononuclear Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3948-3955.

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African Swine Fever Virus Induces Porcine Red Blood Cells Apoptosis and Promotes Phagocytosis of Porcine Peripheral Blood Mononuclear Cells

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