干扰HNRNPAB对牛骨骼肌卫星细胞增殖与分化的作用研究

doi:10.11843/j.issn.0366-6964.2022.10.014

Abstract

Abstract: The study aimed to explore the effect of HNRNPAB (heterogeneous nuclear ribonucleoprotein AB) on the proliferation and differentiation of bovine skeletal muscle satellite cells. In this study, primary skeletal muscle satellite cells of Luxi cattle fetus isolated and cultured in vitro were used as experimental materials. The myogenic differentiation in vitro was induced, and the cells before differentiation and on day 1, 2, and 3 after differentiation were obtained. RNA (4 replicates per group) or protein (3 replicates per group) were extracted, qRT-PCR and Western blot were used to detect the expression of HNRNPAB before and after myogenic differentiation. Then the interfering RNA of HNRNPAB was synthesized and transfected into bovine skeletal muscle satellite cells to interfere with the expression of HNRNPAB, and a negative control group was set up; In the experimental group and the control group, EdU was used to detect cell proliferation, qRT-PCR and Western blot were used to detect the mRNA and protein expression levels of HNRNPAB, proliferation marker factor Pax7, Cyclin D1 and differentiation marker factor MyoG and MyHC. The mRNA expression of HNRNPAB in the process of myogenic differentiation of bovine skeletal muscle satellite cells showed a trend of first increase and then decrease, the highest expression was on the first day of differentiation, and the protein expression levels before and after differentiation were also significantly different. After interfering with HNRNPAB, the positive rate of EdU cells increased significantly (P<0.01). The mRNA levels of proliferation marker factor Pax7 and Cyclin D1 significantly decreased compared with the control group (P<0.01). The protein expression level of Pax7 increased significantly (P<0.01). Compared with the control group, the mRNA expression levels of differentiation marker factor MyoG and MyHC were significantly increased (P<0.01), and the protein expression levels of them were also significantly increased (P<0.05). The results of this study showed that interfering with HNRNPAB decreased the transcription level of the proliferation marker factors of bovine skeletal muscle satellite cells, increased the protein expression level of Pax7, and promoted the myogenic differentiation process of bovine skeletal muscle satellite cells.

Key words: HNRNPAB, bovine skeletal muscle, cell proliferation, myogenic differentiation

CLC Number:

S823.81

YANG Guang, YANG Xu, ZI Jingjing, YU Jianjie, GUO Hong, DING Xiangbin, LIU Xinfeng, ZHANG Linlin. Effects of Interfering HNRNPAB on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3412-3420.

References 32

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Effects of Interfering HNRNPAB on Proliferation and Differentiation of Bovine Skeletal Muscle Satellite Cells

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