Kruppel样因子15对和盈黑鸡前体脂肪细胞增殖分化的影响

doi:10.11843/j.issn.0366-6964.2022.07.009

Abstract

Abstract: The purpose of this study was to clarify the tissue and cell expression pattern of KLF15 in Heying Black chickens and to elucidate the effects of interfering KLF15 on the proliferation and differentiation of preadipocytes of Heying Black chickens. RT-qPCR was used to detect the expression of KLF15 in various tissues, the developmental expression pattern in abdominal fat tissues and the expression level in cells at different stages of adipogenic induction differentiation. After interfering with KLF15, cell proliferation was detected by CCK-8 and EdU, triglyceride content was analyzed, Oil red O staining was carried out, and marker genes for adipocyte differen-tiation were detected (PPARγ, C/EBPa and FAS). The effects of interfering with KLF15 on adipogenic differentiation of Heying Black chicken preadipocytes were investigated from morphological and molecular biological perspectives. The results showed that KLF15 was widely expressed in all detected tissues of Heying Black chickens, with higher expression levels in liver, leg muscle and abdominal fat tissues. KLF15 showed "L" and "U" shaped expression trend in abdominal fat of males and females, respectively, and the expression peak appeared at one day of age. KLF15 mRNA increased slowly in the early stage of cell differentiation, and its expression peak occurred in the late stage of cell differentiation. Cell proliferation detection showed that the results of CCK-8 and EdU exhibited the same trend, and interfering with KLF15 highly significantly inhibited the proliferation of preadipocytes (P<0.01), obviously reduced the accumulation of lipid droplets in preadipocytes, extremely significantly reduced triglyceride content (P<0.01). In addition, the mRNA levels of PPARγ and C/EBPa were extremely significantly down-regulated (P<0.01), and FAS mRNA levels were significantly down-regulated (P<0.05). This study revealed the expression pattern of KLF15 in Heying Black chickens and verified that interfering with KLF15 could inhibit the proliferation and differentiation of preadipocytes. It is speculated that KLF15 is a negative regulator of preadipocytes, it possibly regulate the differentiation of preadipocytes through regulating the expression of PPARγ, C/EBPa and FAS genes.

Key words: Heying Black chicken, KLF15, preadipocytes, interference, cell proliferation and differentiation

CLC Number:

S831.2

CHEN Lan, ZHANG Tao, DING Hao, XIE Kaizhou, ZHANG Genxi, WANG Jinyu. Effects of Kruppel-like Factor 15 Gene on Proliferation and Differentiation of Preadipocytes of Heying Black Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2118-2129.

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Effects of Kruppel-like Factor 15 Gene on Proliferation and Differentiation of Preadipocytes of Heying Black Chickens

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