两株不同J亚群禽白血病毒株诱发组织增生病变和细胞因子表达的比较

doi:10.11843/j.issn.0366-6964.2022.06.023

Abstract

Abstract: The aim of this study was to compare the differences of pathogenicity, innate immune factors and oncogenic factors expressions of chickens induced by two different avian leukosis virus subgroup J (ALV-J) strains. Seventy-five 1-day-old chickens were inoculated with 10³ TCID50 acute fibrosarcoma ALV-J strain (ALV-J-SD1005), 10³ TCID50 myeloma ALV-J strain (ALV-J-NX0101), and same dose sterile PBS saline. The body weight, tissue hyperplasia lesions, mortality, viral loads in blood and subcutaneous fibrous tissues, innate immune factors (MDA5, IRF7, IFN-α/β) and cell proliferation-related factors (TRIM25, P53, 14-3-3σ, STAT1) of the infected chickens were detected at 7 days post inoculation (dpi), 14 dpi, 21 dpi. The results showed that the fibrosarcoma in ALV-J-SD1005 group was firstly observed at 10 dpi, the fibrosarcoma incidence was 100% (18/18) and mortality was 5.2% (1/19) at 14 dpi, and the sarcoma index and the mortality increased continuously from 14 dpi to 21 dpi, and respectively reached 34.4%(74.5/209.5) and 58.3% (7/12) at 21 dpi. Viral loads in blood and subcutaneous fibrous tissues were also significantly increased. Meanwhile, the mRNA expressions of MDA5, IRF7, P53 in the livers were significantly up-regulated, and the mRNA expressions of IFN-α/β and 14-3-3σ genes were significantly down-regulated, while chicken TRIM25 was significantly down-regulated at the early stage of virus infection (at 7 dpi), and significantly up-regulated at the late stage (14~21 dpi). In ALV-J-NX0101 group, the tissue hyperplasia lesions and death were not observed within 21 days after infection, but the viral loads increased significantly, the mRNA expressions of TRIM25, MDA5, IRF7 and IFN-α/β genes were decreased significantly, and the expression of STAT1 gene was up-regulated significantly. It can be concluded that ALV-J-SD1005 strain and ALV-J-NX0101 strain can cause significantly different antiviral immune responses and antitumor responses during their infections, which result into dramatically different virus proliferation and pathogenicity. This study provides scientific basis for further undestanding the tumorigenic mechanism of two ALV-J viruses and exploring new diagnostic markers.

Key words: ALV-J, chicken, fibrous tissue hyperplasia, cell proliferation-related factors, TRIM25

CLC Number:

S852.65

LIU Junhong, ZHAO Yue, LI Hongmei, WANG Ying, WANG Moyu, CHENG Ziqiang, QIU Jianhua, HOU Qiuling, GUO Huijun. Comparison of Tissue Hyperplasia Lesions and Cytokines' Expressions Induced by Two Different Avian Leukosis Virus Subgroup J Strains[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1895-1904.

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Comparison of Tissue Hyperplasia Lesions and Cytokines' Expressions Induced by Two Different Avian Leukosis Virus Subgroup J Strains

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