茵山莲水提物对四氯化碳诱导小鼠急性肝损伤的保护作用

doi:10.11843/j.issn.0366-6964.2022.07.030

Abstract

Abstract: The aim of this study was to explore the protective effect and possible mechanism of aqueous extract of Yinshanlian (YSL) on acute liver injury induced by carbon tetrachloride (CCl₄) in mice, so as to provide theoretical guidance for further research and clinical transformation of YSL. Sixty ICR mice were randomly divided into 6 groups (n=10):control group, model group, low (1.17 mg·g^-1), medium (2.34 mg·g^-1) and high (4.68 mg·g^-1) YSL dosage groups. The drugs were administered by gavage once a day for 7 days. One hour after the last administration, except the control group, the mice in other groups were injected intraperitoneally with 0.2% CCl₄ to establish the model of acute liver injury. After 12 hours, blood was collected from mouse eyeballs, serum was isolated, and liver tissue was collected. The serum total protein (TP) and activity of glutathione S transferase (GST), as well as the activity of GST, succinate dehydrogenase (SDH), catalase (CAT) and total superoxide dismutase (T-SOD) in liver tissue were detected by biochemical analysis. The liver pathology and liver fibrosis were observed by HE staining and Masson staining. Expression and localization of IL-1β, tumor necrosis factor alpha (TNF-α), angiogenesis factor A (VEGFA), matrix metalloproteinase 9 (MMP9) and transcription factor NF-κBp65, protein kinase IKK, and p38MAPK in liver tissue were detected by Western blot and immunohistochemistry. The results showed that 2.34 mg·g^-1 YSL aqueous extract could significantly reduce the level of serum GST and significantly increase the level of intrahepatic CAT compared with the model group. There was no significant difference in T-SOD among these groups. There was no significant difference in the levels of liver GST, SDH and serum TP between 2.34 mg·g^-1 YSL aqueous extract group and control group or model group. Besides, 2.34 mg·g^-1 YSL aqueous extract could alleviate liver pathological injury and fibrosis induced by CCl₄, and decrease the expression of liver IL-1β, TNF-α, VEGFA, NF-κBp65 and IKK. It is suggested that the aqueous extract of YSL probably inhibits liver oxidative stress and inflammation through NF-κB and p38MAPK pathways to alleviate acute liver injury in mice.

Key words: aqueous extract of Yinshanlian, acute liver injury, CCl₄, mechanism

CLC Number:

S853.74

WEI Yuanyuan, FAN Yimeng, YUAN Yanyan, GA Yu, ZHANG Yannan, HAN Juncheng, HAO Zhihui. Protective Effect of Aqueous Extract of Yinshanlian on Acute Liver Injury Induced by Carbon Tetrachloride in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2333-2342.

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Protective Effect of Aqueous Extract of Yinshanlian on Acute Liver Injury Induced by Carbon Tetrachloride in Mice

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