奶牛乳腺炎差异表达lncRNA BCL2对炎症及凋亡相关mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2022.07.013

Abstract

Abstract: The aim of this study was to investigate the role of long non-coding RNA BCL2 (lncRNA BCL2) in inflammation and apoptosis of dairy cow mammary epithelial cells. The lncRNA BCL2 sponge adsorbed miRNAs and the mRNAs they targeted were analyzed using RNAhybrid, Targetscan and miRwalk softwares, and KEGG pathway enrichment analysis was performed on these target genes; lncRNA BCL2 expression in mammary tissue and mammary epithelial cell inflammation and its effect on the expression of genes involved in inflammation and apoptosis in mammary epithelial cells were studied using qPCR and lncRNA super expression technology. The results showed that, compared with control group, the lncRNA BCL2 was significantly down-regulated (P<0.05) in mammary gland tissues of clinical mastitis cow and inflammatory mammary epithelial cells induced by LPS. In the presence of lncRNA BCL2 overexpression, the expression of the inflammatory cytokines IL-1βand IL-8 and the key gene NF-κB (p65/p50) of the inflammatory signaling pathway were significantly up-regulated (P<0.05). The expression of CASP3 and CASP9 genes related to apoptosis were significantly decreased (P<0.05), BCL2 expression was significantly up-regulated (P<0.001), and the ratio of BCL2 to BAX was greater than 2. The above results were consistent with the results of bioinformatics analysis, which indicated that lncRNA BCL2 might regulate inflammation and apoptosis of bovine mammary epithelial cells through lncRNA BCL2-miRNA-BCL2 (NLRP1, CASP1, NLRP3) network. It can conclude from the above results that in the process of cow mastitis, the expression of lncRNA BCL2 is down-regulated, which weakens its effect on increasing the expression of IL-1β, IL-8, and NF-κB, subsequently alleviates mammary inflammation. At the same time, the decrease of lncRNA BCL2 expression attenuates its role in down-regulating CASP3 and CASP9 gene expression and up-regulating BCL2 gene expression, which might promote the apoptosis of cow mammary epithelial cells.

Key words: lncRNA BCL2, mammary epithelial cells, cellular inflammation, apoptosis, mastitis

CLC Number:

JIAO Peng, WANG Xingping, WANG Shuzhe, WANG Jinpeng, LUORENG Zhuoma, JIA Li, WEI Dawei, MA Yun. Effect of Differentially Expressed lncRNA BCL2 in Dairy Cow with Mastitis on the Expression of Inflammation and Apoptosis Related mRNA[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2160-2171.

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Effect of Differentially Expressed lncRNA BCL2 in Dairy Cow with Mastitis on the Expression of Inflammation and Apoptosis Related mRNA

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