北京黑猪NR6A1、VSX2、VRTN、LTBP2基因多态性与脊椎数及胴体性状的关联分析

doi:10.11843/j.issn.0366-6964.2022.06.033

Abstract

Abstract: The aim of this study was to investigate the association between polymorphisms of NR6A1, VSX2, VRTN and LTBP2 genes and vertebral number and carcass traits (carcass length, straight length, diagonal length and carcass weight) in Beijing black pigs. In this study, DNA was extracted from ear tissue of 410 healthy Beijing black pigs at the age of (210±7) days. The exon regions of 4 genes were genotyped by PCR and Sanger sequencing. The distribution of genotype frequency and allele frequency of the mutated loci were calculated, and the correlation between polymorphisms of mutated loci and vertebral number and carcass traits was analyzed. A total of 15 SNPs were detected in VSX2, VRTN and LTBP2 genes, including one synonymous mutation in CDS region and 14 3'UTR mutations. Statistical analysis using Duncan's multiple test revealed that a mutation of VSX2 c.536 G > A was significantly associated with vertebral number (P < 0.05). Four mutations in VRTN 3'UTR were significantly associated with traits (P < 0.05). Two mutations, c.2598 A > G and c.2607 G > T, were significantly related with vertebral number, carcass length, carcass straight length and carcass diagonal length(P < 0.05). A mutation, c.3087 G > C, was significantly associated with carcass diagonal length(P < 0.05). A mutation, c.3094 A > G, was significantly related with vertebral number and carcass diagonal length(P < 0.05). Six mutants in LTBP2 3'UTR, c.7158 A > G, c.6869 C > T, c.6319 G > C, c.6246 G > T, c.6170 A > G, c.6079 G > T, were significantly associated with vertebral number (P < 0.05), but no significant association with carcass traits. In conclusion, 10 SNPs in VSX2, VRTN and LTBP2 genes were significantly associated with vertebral number (P < 0.05), 4 SNPs in VRTN were significantly associated with carcass traits (P < 0.05). Above all SNPs could be used as candidate functional loci for the variation of vertebral number and carcass traits of Beijing black pigs.

Key words: Beijing black pigs, gene, vertebral number, carcass traits, association

CLC Number:

S828.2

NIU Naiqi, LIU Qian, HOU Xinhua, LIU Xin, WANG Ligang, ZHAO Fuping, GAO Hongmei, SHI Lijun, WANG Lixian, ZHANG Longchao. Association of Polymorphisms of NR6A1, VSX2, VRTN, LTBP2 Genes with Vertebral Number and Carcass Traits in Beijing Black Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 2005-2014.

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Association of Polymorphisms of NR6A1, VSX2, VRTN, LTBP2 Genes with Vertebral Number and Carcass Traits in Beijing Black Pigs

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