犊牛腹泻粪便样本中纽布病毒VP1和RNA依赖性RNA聚合酶基因的序列分析

doi:10.11843/j.issn.0366-6964.2021.08.029

Abstract

Abstract: Nebovirus (NeV) is an emerging diarrhea-causing pathogen in calves in China. Its VP1 protein contains receptor binding sites and neutralizing antigen epitopes, which are closely related to virus infection and immunity. The purpose of this study was to analyze the molecular characteristics of VP1 genotype 1.2 strains. In this study, diarrhea fecal samples of calves were collected from Ningxia and Henan in 2019 for detecting NeV by RT-PCR, and the complete major capsid proteins (VP1) and RNA-dependent RNA polymerase (RdRp) were amplified from tested positive samples. The results showed that the NeV detection rates in Ningxia and Henan were 11.32% and 8.62%, respectively. The complete VP1 and RdRp sequences of genotype 1.2 were successfully obtained from 4 positive samples, which shared 75.4%-97.8% amino acid identity with 73 complete VP1 sequences available in GenBank. Compared with all other 3 VP1 of genotype 1.2 in GenBank, the 4 strains in this study shared 1 identical amino acid variation in P2 domain and 2 identical amino acid variations in P1 domain. Compared with genotype 1.1, 1.3 and 1.4 strains from China, the 4 strains shared 9, 18 and 14 identical amino acid variations in P2 domain, respectively, and shared 2 identical amino acid variations in P1 domain and 1 identical amino acid variations in S domain, respectively. The 4 complete RdRp sequences in this study were NB-like genotype and shared 67.2%-94.8% nucleotide identity with all other 8 complete RdRp sequences in GenBank. This is firstly detection of VP1 1.2 genotype NeV strains in China, and 4 complete VP1 and RdRp sequences of genotype 1.2 strains were successfully obtained, contributing to better understanding molecular prevalence of NeVs.

Key words: Nebovirus, detection, VP1, RdRp, genotype 1.2

CLC Number:

S852.659.6

LI Siyi, YUE Hua, TANG Cheng. Sequences Analysis of Nebovirus VP1 and RNA-dependent RNA Polymerase in Fecal Samples from Calf with Diarrhea[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2354-2360.

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Sequences Analysis of Nebovirus VP1 and RNA-dependent RNA Polymerase in Fecal Samples from Calf with Diarrhea

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