犬慢性肾衰竭进程中肠道菌群代谢物短链脂肪酸水平的变化及其对肾功能的影响

doi:10.11843/j.issn.0366-6964.2021.08.027

Abstract

Abstract: This study aimed to evaluate the levels of short chain fatty acids in dogs with chronic renal failure and healthy dogs, and explore the causes of changes in short chain fatty acids and its impact on renal function. Twenty-two dogs with mild chronic renal failure (M-CRF group), 29 dogs with severe chronic renal failure (S-CRF group) and 26 healthy dogs (HC group) were selected. The diversity of gut microbiota was analyzed by 16S rDNA sequencing technology and the concentration of short chain fatty acids in feces was detected by gas chromatography. The effects of gut microbiota and sodium butyrate on renal function of 5/6 nephrectomized dogs were observed by fecal bacteria transplantation and sodium butyrate supplementation. The results showed that:1) the observed species and Simpson index of gut microflora diversity in S-CRF group were lower than those in HC group (P<0.05). PCoA analysis showed that the gut microflora in S-CRF group was different from that both in M-CRF and HC group. 2) Lefse analysis showed that there were a large number of different flora between S-CRF group and HC group. Seven species (bacteroideae, Bacteroides, Pseudomonas, et al) were enriched in S-CRF group, while eleven species, such as Prevotellaceae, Clostridiaceae, Prevotella, Faecalibacterium and so on, were enriched in HC group. CCA analysis showed that the species richness in S-CRF group and HC group were positively correlated and negatively correlated with renal function indexes, respectively. 3) The fecal concentrations of acetic acid, propionic acid and butyric acid in S-CRF group were significantly lower than those in HC group and M-CRFgroup, and butyric acid concentration in M-CRF group was significantly lower than that in HC group too (P<0.05). Furthermore, butyric acid concentration was negatively correlated with serum cystatin C (Cys-C), creatinine (Cr) and urea nitrogen (BUN),and the correlation coefficients were -0.451, -0.583 and -0.514 (P<0.01), respectively. 4) Compared with chronic renal failure model group (5/6 Nx group), the serum Cr and BUN level of 5/6 nephrectomized dog fed sodium butyrate were significantly decreased after 8 weeks (P<0.05). Serum Cr and BUN level of 5/6 nephrectomized mice transplanted fecal bacteria from dogs with CRF were more higher than those in 5/6 Nx group after 8 weeks (P<0.05), while sodium butyrate could reverse these changes. In summary, chronic renal failure of dogs can lead to the decrease of gut microflora diversity, the change of flora structure and abundance, and the decrease of fecal short chain fatty acids concentration. It provides a new theoretical basis for the prevention and treatment of chronic renal failure in dogs.

Key words: chronic renal failure, dog, gut microbiota, short-chain fatty acids, renal function

CLC Number:

S856.59

LIU Jing, ZHU Daoxian, LU Jinye, ZHANG Yiduo, LU Wei, LU Jiang. Alteration of Short-Chain Fatty Acids Produced by Gut Microflora in Dogs with Chronic Renal Failure and Its Effect on Renal Function[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2334-2343.

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Alteration of Short-Chain Fatty Acids Produced by Gut Microflora in Dogs with Chronic Renal Failure and Its Effect on Renal Function

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