三氧化二砷对鸡肝脏氧化应激和蛋氨酸亚砜还原酶表达的影响

doi:10.11843/j.issn.0366-6964.2020.11.025

Abstract

Abstract: This study aimed to investigate the effect of arsenic trioxide (ATO) on oxidative stress and methionine sulfoxide reductase (Msrs) expression in chicken liver. A total of 32 one-day-old chicks were divided into control group (saline solution), low-dose group (1 mg·kg^-1 ATO solution), medium-dose group (3 mg·kg^-1 ATO solution) and high-dose group (9 mg·kg^-1 ATO solution). Chickens were treated by gavage with ATO solution of corresponding concentration once a day for 5 weeks. The liver coefficient, histopathological sections, MDA level, SOD activity, Msrs gene and protein expression levels of liver were analyzed. The results showed that compared with the control group, the liver coefficient of the medium- and high-dose groups were significantly increased (P<0.01), and the liver congestion and vesicular degeneration were found in the medium- and high-dose groups. Meanwhile, the experimental groups had significantly increased MDA levels (P<0.01) and decreased SOD activity (P<0.01) as compared with the control group; MsrA, MsrB1, MsrB3 gene mRNA expression levels in the ATO treatment groups were significantly increased compared with the control group (P<0.01 or P<0.001); However, the protein levels of MsrA were reduced significantly (P<0.01). The results indicated that ATO induced oxidative stress in the liver of chickens, and at the same time promoted the expression of Msrs in liver to clear ROS and reduce liver oxidative damage.

Key words: arsenic trioxide, Msrs, chicken, liver, oxidative stress

CLC Number:

S856.9

WU Shaofeng, ZHONG Gaolong, WAN Fang, NING Zhijun, JIANG Xuanxuan, HU Ting, CHEN Zhongwei, ZHAO Wu, LU Bingxia, TANG Zhaoxin, HE Ying, HU Lianmei. Effects of Arsenic Trioxide Treatment on Oxidative Stress and Methionine Sulfoxide Reductase Expression in Chicken Liver[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2867-2874.

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Effects of Arsenic Trioxide Treatment on Oxidative Stress and Methionine Sulfoxide Reductase Expression in Chicken Liver

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