ChMDA5通路参与传染性法氏囊病病毒致雏鸡法氏囊损伤的研究

doi:10.11843/j.issn.0366-6964.2020.10.024

Abstract

Abstract: Infectious bursal disease virus (IBDV), a double-stranded RNA virus, can cause the infection of chickens with bursal lesions, leading to immunosuppression; melanoma differentiation-associated gene 5 (MDA5) is a pattern recognition receptor that specifically recognises double-stranded RNA viruses. In order to investigate the mechanism of the chicken MDA5 (chMDA5) signalling pathway in pathological injury in IBDV-infected chickens, fifty 14-day-old specific pathogen-free (SPF) chickens were allocated into 2 groups (infected and control) and twenty-five chickens were in each group. Each chicken of infected group was inoculated with 0.6 mL IBDV JIC7 virus solution intraocularly and intranasally. The chickens in control group were administered sterile phosphate buffered saline (PBS) in the same manner simultaneously. The chickens’ bursa of Fabricius was isolated at the 1st, 4th, 7th, 21st and 35th day after infection. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the IBDV load, and mRNA expression of chMDA5, chMDA signal pathway adaptor protein (chIPS-1), transcription factor (chIRF3, chNF-κB) and downstream product cytokines (chIFN-β, chTNF-α, chIL-1β and chIL-6). Indirect immunofluorescence assay was performed for the expression of chMDA5 protein in chicken bursa of Fabricius. Conventional pathological methods were used to examine the pathological changes in the bursa of Fabricius. The results showed that the expression of chMDA5 as well as chIPS-1, chIRF3, chNF-κB, chIFN-β, chTNF-α, chIL-1β and chIL-6 were significantly higher than those in the control group, and morphological damage occurred in the bursal tissue in the infected group. The aforementioned trend was consistent with the change in IBDV load. The results indicated that chMDA5 and its signal transduction pathway could be activated by IBDV, which involves the bursa of Fabricius injury and anti-injury process in chickens infected with IBDV.

Key words: IBDV, bursa of Fabricius, chMDA5 signal transduction, pathology morphological damage, chicken

CLC Number:

S852.65⁺9.4

LUAN Ya'nan, XU Danlei, GE Ming, TANG Zequn, ZHAO Xia, ZHANG Ruili. Study on the Involvement of Chicken Melanoma Differentiation-Associated Gene 5 Pathway in Bursa Injury of Chicken Induced by Infectious Bursal Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2557-2566.

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Study on the Involvement of Chicken Melanoma Differentiation-Associated Gene 5 Pathway in Bursa Injury of Chicken Induced by Infectious Bursal Disease Virus

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