1株H1N1亚型猪流感病毒的全基因组特征及其对小鼠的致病性

doi:10.11843/j.issn.0366-6964.2020.07.030

Abstract

Abstract: This study aimed to understand the epidemiological situation of swine influenza virus (SIV) and analyze its evolutionary and genomic characterization in Henan province. In April 2018, 150 nasal swabs were collected from a pig herd with suspected influenza clinical signs to isolate the virus. The isolated virus was sequenced and analyzed. The pathogenicity was evaluated by infecting BALB/c mice. A strain of H1N1 subtype SIV was isolated and named of A/swine/Henan/NY20/2018(H1N1). Phylogenetic analysis results demonstrated that HA and NA genes of the isolated virus belong to the Eurasian avian H1N1 lineage, PB2, PB1, PA, NP and M genes belonged to the pdm/09 H1N1 lineage, and NS gene belonged to the classical swine H1N1 lineage. The HA protein cleavage site of the isolated is PSIQSR↓GL, which accords with the molecular characteristics of low pathogenic avian influenza virus. It can effectively replicate in the lungs and turbinates of mice and can cause pathological changes in lung tissue. A triple reassortant H1N1 subtype virus was isolated in this study, which has a certain pathogenicity to mice, suggesting that further monitoring of SIV should be strengthened.

Key words: swine influenza virus, H1N1 subtype, pathogenicity

CLC Number:

S852.659.5

ZHAO Yuzhong, DING Guofei, LIU Jiaqi, LI Li, LI Yingchao, WANG Bin, SHAO Qingyuan, FENG Jian, GUO Lihong, LIU Sidang, XIAO Yihong. Genomic and Evolutionary Characterization of a H1N1 Swine Influenza Virus and Its Pathogenicity in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1768-1774.

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Genomic and Evolutionary Characterization of a H1N1 Swine Influenza Virus and Its Pathogenicity in Mice

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