1株H1N1亚型猪流感病毒的全基因组特征及其对小鼠的致病性

doi:10.11843/j.issn.0366-6964.2020.07.030

Abstract

Abstract: This study aimed to understand the epidemiological situation of swine influenza virus (SIV) and analyze its evolutionary and genomic characterization in Henan province. In April 2018, 150 nasal swabs were collected from a pig herd with suspected influenza clinical signs to isolate the virus. The isolated virus was sequenced and analyzed. The pathogenicity was evaluated by infecting BALB/c mice. A strain of H1N1 subtype SIV was isolated and named of A/swine/Henan/NY20/2018(H1N1). Phylogenetic analysis results demonstrated that HA and NA genes of the isolated virus belong to the Eurasian avian H1N1 lineage, PB2, PB1, PA, NP and M genes belonged to the pdm/09 H1N1 lineage, and NS gene belonged to the classical swine H1N1 lineage. The HA protein cleavage site of the isolated is PSIQSR↓GL, which accords with the molecular characteristics of low pathogenic avian influenza virus. It can effectively replicate in the lungs and turbinates of mice and can cause pathological changes in lung tissue. A triple reassortant H1N1 subtype virus was isolated in this study, which has a certain pathogenicity to mice, suggesting that further monitoring of SIV should be strengthened.

Key words: swine influenza virus, H1N1 subtype, pathogenicity

CLC Number:

S852.659.5

ZHAO Yuzhong, DING Guofei, LIU Jiaqi, LI Li, LI Yingchao, WANG Bin, SHAO Qingyuan, FENG Jian, GUO Lihong, LIU Sidang, XIAO Yihong. Genomic and Evolutionary Characterization of a H1N1 Swine Influenza Virus and Its Pathogenicity in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1768-1774.

References 40

[1]	FONI E, GARBARINO C, CHIAPPONI C, et al. Epidemiological survey of swine influenza A virus in the wild boar population of two Italian provinces[J]. Influenza Other Respir Viruses, 2013, 7(S4):16-20.
[2]	WEBSTER R G, BEAN W J, GORMAN O T, et al. Evolution and ecology of influenza A viruses[J]. Microbiol Rev, 1992, 56(1):152-179.
[3]	MUKHERJEE T R, AGRAWAL A S, CHAKRABARTI S, et al. Full genomic analysis of an influenza A (H1N2) virus identified during 2009 pandemic in Eastern India:evidence of reassortment event between co-circulating A(H1N1)pdm09 and A/Brisbane/10/2007-like H3N2 strains[J]. Virol J, 2012, 9:233.
[4]	FOUCHIER R A M, MUNSTER V, WALLENSTEN A, et al. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls[J]. J Virol, 2005, 79(5):2814-2822.
[5]	TONG S X, ZHU X Y, LI Y, et al. New world bats harbor diverse influenza A viruses[J]. PLoS Pathog, 2013, 9(10):e1003657.
[6]	TONG S X, LI Y, RIVAILLER P, et al. A distinct lineage of influenza A virus from bats[J]. Proc Natl Acad Sci U S A, 2012, 109(11):4269-4274.
[7]	YANG H L, CHEN Y, QIAO C L, et al. Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses[J]. Proc Natl Acad Sci U S A, 2016, 113(2):392-397.
[8]	ZHAO N, MARTIN B E, YANG C K, et al. Association analyses of large-scale glycan microarray data reveal novel host-specific substructures in influenza A virus binding glycans[J]. Sci Rep, 2015, 5:15778.
[9]	SUZUKI Y, ITO T, SUZUKI T, et al. Sialic acid species as a determinant of the host range of influenza A viruses[J]. J Virol, 2000, 74(24):11825-11831.
[10]	VAN REETH K, BROWN I H, DVRRWALD R, et al. Seroprevalence of H1N1, H3N2 and H1N2 influenza viruses in pigs in seven European countries in 2002-2003[J]. Influenza Other Respir Viruses, 2008, 2(3):99-105.
[11]	CHUTINIMITKUL S, THIPPAMOM N, DAMRONGWATANAPOKIN S, et al. Genetic characterization of H1N1, H1N2 and H3N2 swine influenza virus in Thailand[J]. Arch Virol, 2008, 153(6):1049-1056.
[12]	KONG W L, HUANG L Z, QI H T, et al. Genetic characterization of H1N2 influenza a virus isolated from sick pigs in Southern China in 2010[J]. Virol J, 2011, 8:469.
[13]	VINCENT A L, PEREZ D R, RAJAO D, et al. Influenza A virus vaccines for swine[J]. Vet Microbiol, 2017, 206:35-44.
[14]	KONG W L, HUANG Y M, CAO N, et al. Isolation and phylogenetic analysis of H1N1 swine influenza virus from sick pigs in Southern China[J]. Indian J Virol, 2011, 22(1):66-71.
[15]	ZHU W F, YANG S, GUO Y J, et al. Imported pigs may have introduced the first classical swine influenza viruses into Mainland China[J]. Infect Genet Evol, 2013, 17:142-146.
[16]	ZHU H, WEBBY R, LAM T T, et al. History of Swine influenza viruses in Asia[J]. Curr Top Microbiol Immunol, 2013, 370:57-68.
[17]	FAN X H, ZHU H C, ZHOU B P, et al. Emergence and dissemination of a swine H3N2 reassortant influenza virus with 2009 pandemic H1N1 genes in pigs in China[J]. J Virol, 2012, 86(4):2375-2378.
[18]	WEBSTER R G, ROTT R. Influenza virus a pathogenicity:the pivotal role of hemagglutinin[J]. Cell, 1987, 50(5):665-666.
[19]	KLENK H D, ROTT R. The molecular biology of influenza virus pathogenicity[J]. Adv Virus Res, 1988, 34:247-281.
[20]	HORIMOTO T, KAWAOKA Y. Pandemic threat posed by avian influenza A viruses[J]. Clin Microbiol Rev, 2001, 14(1):129-149.
[21]	刘清政, 王宏宇, 杜以军, 等. 一株猪流感病毒的遗传进化分析及其致病力研究[J]. 中国预防兽医学报, 2017, 39(12):1022-1025.LIU Q Z, WANG H Y, DU Y J, et al. Genetic evolution analysis and pathogenicity of a swine influenza virus[J]. Chinese Journal of Preventive Veterinary Medicine, 2017, 39(12):1022-1025. (in Chinese)
[22]	WANG Z, YANG H L, CHEN Y, et al. A single-amino-acid substitution at position 225 in hemagglutinin alters the transmissibility of Eurasian avian-like H1N1 swine influenza virus in guinea pigs[J]. J Virol, 2017, 91(21):e00800-17.
[23]	李婷, 朱启运, 冉多良, 等. 猪流感病毒分离株A/Swine/Fujian/F1/2001(H5N1)表面蛋白基因序列分析[J]. 新疆农业大学学报, 2006, 29(3):1-4.LI T, ZHU Q Y, RAN D L, et al. Sequence analysis on the surface glycoprotein genes of a swine influenza virus isolate A/Swine/Fujian/F1/2001(H5N1)[J]. Journal of Xinjiang Agricultural University, 2006, 29(3):1-4. (in Chinese)
[24]	ZHU W F, LI L, YAN Z G, et al. Dual E627K and D701N mutations in the PB2 protein of A(H7N9) influenza virus increased its virulence in mammalian models[J]. Sci Rep, 2015, 5:14170.
[25]	HE P, WANG G J, MO Y N, et al. Novel triple-reassortant influenza viruses in pigs, Guangxi, China[J]. Emerg Microbes Infect, 2018, 7(1):85.
[26]	LI X D, LIAO H, LIU Y, et al. Drug-resistant and genetic evolutionary analysis of influenza virus from patients during the 2013 and 2014 influenza season in Beijing[J]. Microb Drug Resist, 2017, 23(2):253-260.
[27]	IKEMATSU H, KAWAI N, IWAKI N, et al. In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against influenza virus isolates in the 2011-2012 season in Japan[J]. J Infect Chemother, 2014, 20(2):77-80.
[28]	GARTEN R J, DAVIS C T, RUSSELL C A, et al. Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans[J]. Science, 2009, 325(5937):197-201.
[29]	ITO T, COUCEIRO J N, KELM S, et al. Molecular basis for the generation in pigs of influenza A viruses with pandemic potential[J]. J Virol, 1998, 72(9):7367-7373.
[30]	GAMMELIN M, MANDLER J, SCHOLTISSEK C. Two subtypes of nucleoproteins (NP) of influenza A viruses[J]. Virology, 1989, 170(1):71-80.
[31]	VAN POUCKE S G, NICHOLLS J M, NAUWYNCK H J, et al. Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution[J]. Virol J, 2010, 7:38.
[32]	NONTHABENJAWAN N, CHANVATIK S, CHAIYAWONG S, et al. Genetic diversity of swine influenza viruses in Thai swine farms, 2011-2014[J]. Virus Genes, 2015, 50(2):221-230.
[33]	SONG Y F, ZHANG Y, ZHANG B, et al. Identification, genetic analysis, and pathogenicity of classical swine H1N1 and human-swine reassortant H1N1 influenza viruses from pigs in China[J]. Viruses, 2020, 12(1):55.
[34]	SMITH G J D, VIJAYKRISHNA D, BAHL J, et al. Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic[J]. Nature, 2009, 459(7250):1122-1125.
[35]	HOWDEN K J, BROCKHOFF E J, CAYA F D, et al. An investigation into human pandemic influenza virus (H1N1) 2009 on an Alberta swine farm[J]. Can Vet J, 2009, 50(11):1153-1161.
[36]	NElSON M I, VINCENT A L, KITIKOON P, et al. Evolution of novel reassortant A/H3N2 influenza viruses in North American swine and humans, 2009-2011[J]. J Virol, 2012, 86(16):8872-8878.
[37]	VIJAYKRISHNA D, POON L L M, ZHU H C, et al. Reassortment of pandemic H1N1/2009 influenza A virus in swine[J]. Science, 2010, 328(5985):1529.
[38]	NELSON M I, VINCENT A L. Reverse zoonosis of influenza to swine:new perspectives on the human-animal interface[J]. Trends Microbiol, 2015, 23(3):142-153.
[39]	WATSON S J, LANGAT P, REID S M, et al. Molecular epidemiology and evolution of influenza viruses circulating within European swine between 2009 and 2013[J]. J Virol, 2015, 89(19):9920-9931.
[40]	LEWIS N S, RUSSELL C A, LANGAT P, et al. The global antigenic diversity of swine influenza A viruses[J]. eLife, 2016, 5:e12217.

Genomic and Evolutionary Characterization of a H1N1 Swine Influenza Virus and Its Pathogenicity in Mice

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