我国猪群中多杀性巴氏杆菌的基因型分析

doi:10.11843/j.issn.0366-6964.2019.05.017

Abstract

Abstract:

The aim of this study was to understand the genotypical characteristics of swine P. mul-tocida currently circulating in China. Capsular genotyping, LPS genotyping, MLST genotyping were performed on P. multocida isolates from the clinical samples including lungs, nasal swabs, tracheas, livers, and other tissues of pigs with respiratory disorders in different farms in different regions of mainland China; virulence genotyping based on the detection of different virulence gene profiles was also set for the isolates recovered herein. The results showed that the capsular genotypes of the swine P. multocida currently circulating in China were A (48.85%), D (42.75%), and F (2.67%); the LPS genotypes were L3 (25.00%) and L6 (75.00%); the MLST genotypes were ST3 (21.25%), ST10 (27.50%), ST11 (42.50%), ST12 (2.50%), ST16 (2.50%), ST74 (1.25%), and ST75 (2.50%). When combining the capsular genotypes with the LPS genotypes and the MLST genotypes, it was found that the predominant capsule:LPS:MLST genotypes prevalent in pigs in China were A:L3:ST3 (20.00%), A:L6:ST10 (26.25%), and D:L6:ST11 (42.50%). During the virulence genotyping, some virulence-associated genes were found to show a certain level of "genotype-preference", which means the detection rate of those genes in some genotypes is significantly higher than their detection in other genotypes. These results indicate that a capsule:LPS:MLST genotype D:L6:ST11 of P. multocida is likely to be strongly associated with swine respiratory disease in China.

CLC Number:

S852.612

PENG Zhong, LIANG Wan, AI Weicheng, WANG Fei, HUA Lin, TANG Xibiao, CHEN Huanchun, WU Bin. Genotypical Characteristics of Swine Pasteurella multocida in China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1064-1072.

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Genotypical Characteristics of Swine Pasteurella multocida in China

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