致脑膜炎多杀性巴氏杆菌的分离鉴定及全基因组重测序

doi:10.11843/j.issn.0366-6964.2022.12.020

Abstract

Abstract: This study was designed to investigate the biological characteristics of a meningitis causing Pasteurella multocida and the biological basis for this bacterium causing brain infections. Serum bactericidal tests, intracellular survival tests, mouse infection tests, and nanopore sequencing were performed on a P. multocida strain SD001 which was isolated from the brain of a pig with neurological symptoms. Results were as follows: Compared to those of P. multocida isolates from the other organs of pigs, SD001 displayed a stronger anti-serum bactericidal capacity, and a stronger capacity of surviving in mouse macrophages. In mouse models, SD001 formed a higher bacterial load in both blood and brain than a pneumonic P. multocida strain at the same time points post challenge, and the challenge of SD001 led to inflammatory and other pathological damages in the experimental mice. These findings suggest that SD001 may cause bloodstream infections and meningitis animals. Results from nanopore sequencing revealed that the complete genome sequence of SD001 was 2.45 Mb in size, with an average GC content of 40.25%. The complete genome of SD001 encoded 2 281 putative proteins and 77 RNAs. It also contained two CRISPR sequences, five genomic islands, seven prophages, and 233 virulence factor encoding genes. Genotyping using the complete genome sequence showed that SD001 belonged to capsular: LPS: MLST type A: L6: ST10. SD001 infection could cause meningitis and its strong capacity of anti-serum bactericidal activity as well as inducing bloodstream infection may confer the bacterium capacity of causing brain infections. P. multocida infection should be considered for the diagnosis of brain infections in veterinary clinical settings.

Key words: Pasteurella multocida, meningitis, pig, serum bactericidal test, intracellular survival test, mouse infection test, nanopore sequencing

CLC Number:

S852.612

WANG Fei, YANG Jie, Lü Qingjie, WANG Mixue, LIU Peng, ZHANG Ruoyu, SHI Congcong, WANG Xueying, LIN Lin, HUA Lin, SONG Wenbo, LIANG Wan, CHEN Huanchun, WU Bin, PENG Zhong. Isolation and Genomic Characterization of a Meningitis Causing Pasteurella multocida[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4346-4355.

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Isolation and Genomic Characterization of a Meningitis Causing Pasteurella multocida

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