1株多杀性巴氏杆菌的全基因组序列及致病相关基因分析

doi:10.11843/j.issn.0366-6964.2022.06.019

Abstract

Abstract: This study aimed to investigate the pathogenicity of Pasteurella multocida CS strain by analyzing virulence genes of its complete genome. The bacteria strain was isolated from swine pasteurellosis by conventional methods and its virulence was tested by mice. The complete genome of P. multocida CS strain was sequenced based on the second generation sequencing platform, and analyzed its differences with others submitted to GenBank from different regions and hosts by comparative genomics methods. The LD₅₀ of P. multocida CS strain was 5×10² CFU·mL^-1, which showed strongly toxic to mice. The genomic information of CS strain was submitted to NCBI, and the accession number was SUB11119617. The analysis results of the complete genome sequence of P. multocida CS strain showed that the total genome size of CS strain was 2 599 048 bp, the content of G+C was 39.932%, the CDs encoding gene was 2 580, accounting for 69.6% of the whole genome length. The average length of CDs encoding gene was 952 bp. Besides, there were 53 tRNA and 3 rRNA genes. 87.95% of the coding genes could be assigned as COGs, and the functions of 29 genes were unknown. Phylogenetic analysis using maximum likelihood method showed that CS strain was closely related to 1 pig origin serotype A strain and 2 cattle origin serotype A strain and 1 pig origin serotype D strain. The P. multocida CS strain contained 254 virulence-related genes which were divided into 4 major classes and 11 subclasses. The distribution of several genes related to iron uptake system, adhesion system, secretion system and two-component regulatory system was different among different serotypes. By analyzing the frequency of these gene occurrence of different P. multocida serotypes, it was found that tbpA and iron complex outer membrane receptor protein gene (irp) related with iron uptake system, and ppdD and ompA genes related with adhesion appeared to be associated with bacterial toxic differention among different strains. The secretory system of P. multocida CS strain consisted of three types: type I (hly gene), Tat type and Sec-SRP type. The frequency of hlyD gene among different serotypes was different. P. multocida CS strain's two-component signal transduction system (TCSs) consisted of 16 regulatory genes, 12 induction-related genes and 2 hybrid genes. The relationship between the polymorphism of TCSS genes in different serotypes and virulence of different strains needs to be further studied. To sum up, the gene frequency of iron uptake system, adhesion system, secretion system and two-component regulatory system had polymorphism among different serotypes and different isolates of the same serotypes. These may be one of the reasons for the virulence differences among different P. multocida strains.

Key words: Pasteurella multocida CS strain, complete genome sequence, pathogenomics, polymorphism

CLC Number:

S852.612

XU Wenbo, WU Limei, LIU Xin, LI Sheng, HUANG Fushen, LI Runcheng. Analysis on Complete Genome Sequence and Pathogenic Genes of a Pasteurella multocida Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1858-1869.

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Analysis on Complete Genome Sequence and Pathogenic Genes of a Pasteurella multocida Strain

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