Abstract:

The full genome of waterfowl parvoviruses were amplified by using polymerase chain reaction (PCR) method.The gene fragments were sequenced and analyzed with goose parvovirus (GPV) ZJ，G3，and LJY strains and Muscovy duck parvovirus (MDPV) DK and DYL strains.Sequence analysis demonstrated that NS gene of four isolates is 1 884 bp in length，encoding 627 amino acids，while NS gene of LJY strain has three nucleotide deletions，resulting 626 amino acids in length.The VP gene is 2 199 bp in length，encoding 732 amino acids.The NS protein among GPVs or MDPVs showed 95.9%-99.8% and 96.8%-99.0% homologies，respectively，while the NS between GPVs and MDPVs showed 88.9%-90.4% identities.The VP protein among GPVs or MDPVs showed 90.2%-100.0% and 96.9%-99.3% homologies，respectively，while the VP protein between GPV and MDPV showed only 79.5%-81.6% identities，indicating that antigenicity might be different between GPV and MDPV.Both MDPVs and GPVs have 4 common potential glycosylation sites in NS protein.In VP protein，MDPV DK and DYL strains have 8 potential glycosylation sites，while GPV have only 6.GPV G3 and LJY strains showed deletion of 582-584 NTT and 703-705 NRT potential glycosylation sites，respectively.Phylogenetic analysis of waterfowl parvoviruses indicates that GPVs and MDPVs form two separate evolutionary branches，and recombination does not occur between MDPV and GPV strains.