貉源阿留申病毒VP2和NS1蛋白的抗原性预测

doi:10.11843/j.issn.0366-6964.2020.023

Abstract

Abstract: This study aimed to analyze the epitope characteristics of VP2 and NS1 proteins of raccoon dog and arctic fox amdoparvovirus (RFAV), and further select the conserved B- and T-cell epitopes on the VP2 and NS1 proteins of RFAV in Amdoparvovirus genus. In the present study, the RFAV genome except the 5' and 3' end of the genome was cloned and sequenced, then the bioinformatics programs were used to predict the physical and chemical properties, Secondary structure, post-translational modifications and potential B- and T-cell epitopes of VP2 and NS1 proteins, and further compared the potential epitopes and post-translational modifications with the typical members within Amdoparvovirus genus. The 4 327 bp RFAV genomic sequence in length, encoding 636 amino acids of VP2 protein and 641 amino acids of NS1 protein, was obtained. Both VP2 and NS1 proteins are hydrophilic proteins and the secondary structure were mainly composed of random coils. We predicted there were 3 conserved B-cell epitopes, 3 conserved T-cell epitopes and 8 conserved post-translational modification sites on VP2 protein within the Amdoparvovirus genus; while NS1 protein had 1, 2 and 7 in the respective conserved items. In this study, we cloned the main genome sequence of RFAV, and predicted the potential epitopes and post-translational modifications on VP2 and NS1 proteins of RFAV. This study provides fundamental basis for the development of a candidate vaccine and disease diagnosis of amdoparvoviruses.

Key words: amdoparvovirus, VP2 protein, NS1 protein, epitope, post-translational modification sites

CLC Number:

S852.659.2

WU Yanhong, ZHAO Yongqiang, WEI Tao, ZHANG Xiuting, CONG Li, YUE Zhigang, XIAO Jiamei, SHAO Xiqun. Cloning and Epitopes Analysis of VP2 and NS1 Proteins of Raccoon Dog and Arctic Fox Amdoparvovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1399-1407.

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Cloning and Epitopes Analysis of VP2 and NS1 Proteins of Raccoon Dog and Arctic Fox Amdoparvovirus

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