牛病毒性腹泻病毒E2基因在烟草中的表达

doi:10.11843/j.issn.0366-6964.2014.09.013

Abstract

Abstract:

The current study was undertaken to study expression of E2 gene of bovine viral diarrhea virus (BVDV) in tobacco and to analyze reactionogenicity of the expressed products.A pair of primers was designed according to the major antigen region of the E2 gene derived from GenBank.The plant constitutive expression vector pBI121-E2 containing E2 gene was constructed，and was then transformed into tobacco plants via Agrobacterium tumefaciens.The transgenic anti-type tobacco plants were screened by screening with kanamycin.PCR amplification and real-time PCR analysis indicated that the E2 gene has been a low-copy in five tobacco plants.Western bolt analysis showed that the E2 protein could be specifically recognized by anti BVDV positive serum.Our study provides a basis for further analysis on plants as bioreactors development and the production of E2 oral vaccines.

CLC Number:

S852.659.6

WANG Sheng，XIE Zhi-xun，FAN Qing，XIE Li-ji，DENG Xian-wen，HUANG Li，XIE Zhi-qin，LIU Jia-bo，PANG Yao-shan，LUO Si-si. Expression of E2 Gene of Bovine Viral Diarrhea Virus in Tobacco[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1474-1480.

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Expression of E2 Gene of Bovine Viral Diarrhea Virus in Tobacco

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