Enterobacter cloacae Z0206细菌富硒多糖对Ⅱ型糖尿病KKAy小鼠肾脏病变的影响

doi:10.11843/j.issn.0366-6964.2014.02.021

Abstract

Abstract:

This experiment was conducted to study the effect of the bacterial enriched-selenium polysaccharide enterobacter cloacaeZ0206 on the renal disease of the Type Ⅱ diabetic KKAy mice.Six to eight week-old male KKAy mice were allocated in diabetes group (Model)，Z0206 bacterial enriched-selenium polysaccharide group (CEPS) and Z0206 purified enriched-selenium polysaccharide group (PEPS) based on the blood glucose concentration after two weeks pre-feeding，8 mice in each group.The groups received 0.5 mL distilled water，10 mg·mL^－1 CEPS，10 mg·mL^－1 PEPS respectively.Eight 7-week-old C57/BL mice were used as normal control group (Control).The feeding trial lasted for 42 days.Compared with the control group，the activity of Glutathione Peroxidase (GSH-Px) and Superoxide Dismutase (SOD) in Model group were significantly decreased (P<0.05)，the mRNA transcription of interleukin-1 (IL-1)，interleukin-6 (IL-6)，monocyte chemotactic protein 1 (MCP-1)，nuclear factor-κB (NF-κB)，transforming growth factor-β (TGF-β)，plasminogen activator inhibitor 1 (PAI-1)，plateletderived growth factor β (PDGF-β) and Collagen Type Ⅳ (Col-Ⅳ) were significantly increased (P<0.05).The level of MDA were significantly increased (P<0.05).Compared with the Model group，the enriched-selenium polysaccharide groups resulted in significant repair effect，the activity of GSH-Px and SOD in both polysaccharide groups were significantly increased (P<0.05)，the mRNA transcription of IL-1，IL-6，MCP-1，NF-κB，TGF-β，PAI-1，PDGF-β and Col-Ⅳ were significantly decreased (P<0.05)，the level of MDA were significantly decreased (P<0.05)，matrix proliferation was significantly reduced.It is suggested that the polysaccharide can enhance the antioxidant function of the Type Ⅱ diabetic KKAy mice and repair the damage caused by diabetes to it.

CLC Number:

S856.5

YANG Hang-xian，WANG Feng-qin，ZHOU Xi-hong，WANG Yi-zhen. Effect of Enterobacter cloacae Z0206 Enriched-selenium Polysaccharide on the Renal Disease of the Type Ⅱ Diabetic KKAy Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 313-319.

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Effect of Enterobacter cloacae Z0206 Enriched-selenium Polysaccharide on the Renal Disease of the Type Ⅱ Diabetic KKAy Mice

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