鸡与鹌鹑杂交种早期胚胎与性分化相关的ER等基因的表达

doi:10.11843/j.issn.0366-6964.2014.02.022

Abstract

Abstract:

This study aimed to detect expression of genes related to early embryonic development and sexual differentiation in the chicken-quail hybrids.First，the whole embryos were collected during the hybrid early embryonic development at different times(72，78，84，90，96，102，108，114，120，144，168 and 192 h).Then，based on the identification of early hybrid embryo sexes，Real-time PCR method was used to detect the expressions of sex differentiation associated genes (3β-HSD，P-450c17，P-450arom and ER) during the embryonic development of male and female hybrids at different times.The results showed that in chicken-quail hybrid embryos，both of 3β-HSD and ER genes expression in females were generally significantly higher than that in males.The lowest expression of 3β-HSD occured at 72 h in male，while the highest expression occurred at 96 h in female and male embryos.Within 90 h，the gene expression level of the male and female hybrid embryos P-450c17 was higher，peaking at 78 h，and there were significantly differences(P<0.01) in females and males at each time point within 90 h.P-450arom hybrid embryo gene expression could be detected in females，but the expression of the males could not be detected.There were two peaks，respectively，at 72 and 108 h expression of ER gene in early hybrid embryonic development.

CLC Number:

S813.3

LIU Xian-xia，FENG Xin-lu，ZHAO Zong-sheng，LI Da-quan，GAO Shu. Expression Analysis of Genes Related to Sexual Differentiation during the Early Development of Chicken-Quail Hybrid Embryos[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 320-326.

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Expression Analysis of Genes Related to Sexual Differentiation during the Early Development of Chicken-Quail Hybrid Embryos

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