金黄色葡萄球菌外毒素及荚膜特异性卵黄抗体联合治疗奶牛乳房炎效果研究

doi:10.11843/j.issn.0366-6964.2013.12.023

Abstract

Abstract:

The objective of this study was to evaluate the efficacy of a combination of egg yolk immunoglobulins (IgYs) specific for exotoxins (IgY-toxins) and serotypes 5, 8 and 336 capsules of Staphylococcus aureus (IgY-T5, IgY-T8, IgY-T336, respectively) in the treatment of bovine mastitis. A lactate dehydrogenase assay indicated that IgY-toxins prevented mammary epithelial cells (MAC-T) from lysing. An internalization assay demonstrated that 5 mg·mL^-1 IgY-T336 markedly blocked the invasion of a GFP-expressing S. aureus RN6390 to MAC-T cells, and a combination of 5 mg·mL^-1 of IgY-T336 and 0.5 mg·mL^-1 of IgY-toxins provided the cells with greater protection and improved growth morphology compared with the IgY-T336 treatment alone. A field trial for clinical mastitis indicated that two doses of a combination of 20 mg each of IgY-T5, IgY-T8 and IgY-T336 plus 20 mg of IgY-toxins daily for six days significantly reduced somatic cell counts (SCC) compared with PBS control (P < 0.001). Treatment with IgY-T5, IgY-T8 and IgY-T336 or IgY-toxins alone produced a slight reduction in SCC compared with PBS control. However, the reduction was much lower than that in IgY combination treatment (P < 0.01). Both surface capsules and secreted exotoxins should be targeted in the treatment of S. aureus-caused bovine mastitis with IgY.

CLC Number:

S857.26

WANG Lin-hui, LI Xiao-yu, JIN Li-ji, LI Shu-ying, XU Yong-ping. Efficacy of a Combination of Egg Yolk Immunoglobulins against Staphylococcus aureus Exotoxins and Capsules in the Treatment of Bovine Mastitis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 2016-2021.

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Efficacy of a Combination of Egg Yolk Immunoglobulins against Staphylococcus aureus Exotoxins and Capsules in the Treatment of Bovine Mastitis

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