携带pVAX1-GPV-P32重组减毒沙门菌诱导山羊免疫应答研究

doi:10.11843/j.issn.0366-6964.2013.12.016

Abstract

Abstract:

The aim of this study was to study the immune response effect of the recombinant attenuated Salmonella carrying pVAX1-GPV-P32 to goats. The 60 black goats were randomly divided into three groups as recombinant bacteria group, attenuated vaccine group and the control group, which the goats were fed by the recombinant attenuated Salmonella in recombinant bacteria group, and injected by the goat pox attenuated vaccine in the attenuated vaccine group, but without any treatment in the control group. Then the samples of the blood and respiratory/digestive tract secretion and tissue were collected respectively in the different periods after immunization and used for detection and analysis. The results were showed as follows: (1)The contents of the specific SIgA in recombinant bacteria group was significant higher than that in the attenuated vaccine group (P<0.05) in 7, 15, 30 and 45 d post-immunization (PI), and which reached the highest value (18.172±0.122 μg·L^-1) in 45 d; (2) The contents of anti-GPV P32 antibody in the recombinant bacteria group were slightly lower than that in the attenuated vaccine group, but the difference between groups was not significant (P>0.05); (3)The transformation efficiency of the specific lymphocyte in the recombinant bacteria group was slightly lower than that in the attenuated vaccine group from 0 to15 d PI, but higher than that in the attenuated vaccine group in 30 d and 45 d PI, but the difference was not significant (P>0.05). (4)The IL-2 content of the goat serum in the recombinant bacteria group were less than that in the attenuated vaccine group and the difference was very significant (P<0.01), and the IL-5 levels were higher than that in the attenuated vaccine group and the difference was very significant (P<0.01) too, and the TNF-β levels were higher than that in the attenuated vaccine group and the difference was significant (P<0.05), and the content of IFN-γ and TNF-α were not significant with that in the attenuated vaccine group (P>0.05). These results indicated that the recombinant attenuated Salmonella carrying the pVAX1-GPV-P32 made the goat have a better immune responses.

CLC Number:

XIONG Chao-li, ZHANG Hua, CHENG Zhen-tao, ZHOU Bi-jun,WANG Kai-gong, WEN Ming. Study on Immunity Response in Goat Induced by the Attenuated Salmonella typhimurium Carrying pVAX1-GPV-P32[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1970-1975.

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Study on Immunity Response in Goat Induced by the Attenuated Salmonella typhimurium Carrying pVAX1-GPV-P32

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