11味中药多糖提取物对不同MHC B-LβⅡ基因型鸡新城疫抗体水平和IFN-γ、IL-4含量的影响

doi:10.11843/j.issn.0366-6964.2013.10.024

Abstract

Abstract:

In order to explore the effects of polysaccharide extracted from 11 kinds of traditional Chinese medicines on immunomodulatory in different MHC B-LβⅡ genotype chickens, PCR-RFLP technique was applied to group one-day-old male liangfeng Green-Feet Ma chickens according to different MHC B-LβⅡ genotypes, then they were given high, middle and low dose of polysaccharides extracted from traditional Chinese medicines and saline, respectively. The effects of traditional Chinese medicinal polysaccharide extraction on antibody response kinetics to ND vaccine and IFN-γ and IL-4 contents in serum were determined by ELISA method. The results showed that: (1) Traditional Chinese medicinal polysaccharide extraction at different dose could improve ND antibody level, IFN-γ and IL-4 contents in each MHC B-LβⅡ genotype chicken, and in high dose traditional Chinese medicinal polysaccharide extraction, AA genotype chicken were higher than that of AB, BB genotype, in middle and low dose traditional Chinese medicinal polysaccharide extraction, AB genotype were higher than that of AA, BB genotype; (2) The ND antibody level and IL-4 contents of AA genotype chicken in high dose traditional Chinese medicinal polysaccharide extraction were higher than that in middle and low dose group, the ND antibody level, IFN-γ and IL-4 contents of AB, BB genotype chicken in middle dose traditional Chinese medicinal polysaccharide extraction were higher than that in high and low dose group. These results indicated that the polysaccharide extracted from 11 kinds of traditional Chinese medicines could stimulate ND antibody production and cytokines IFN-γ, IL-4 excretion in different MHC B-LβⅡ genotype chickens, and the optimum immunomodulatory dose were different in each MHC B-LβⅡ genotype chicken.

CLC Number:

S853.7

ZHU Xiao-qing, LI Xiao-zhen, QIAO Hai-bo, JIA Shu-hong, ZHANG Dong-sheng, GU Xin-li. Effects of Polysaccharide Extracted from 11 Kinds of Traditional Chinese Medicines on ND Antibody Level and IFN-γ, IL-4 Contents in Different MHC B-LβⅡ Genotype Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1685-1692.

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Effects of Polysaccharide Extracted from 11 Kinds of Traditional Chinese Medicines on ND Antibody Level and IFN-γ, IL-4 Contents in Different MHC B-LβⅡ Genotype Chickens

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