Acta Veterinaria et Zootechnica Sinica ›› 2023, Vol. 54 ›› Issue (12): 5275-5292.doi: 10.11843/j.issn.0366-6964.2023.12.036

• CLINICAL VETERINARY MEDICINE • Previous Articles     Next Articles

Composition Analysis of Seed of Areca catechu L. in Deep Processing based on UHPLC-QE-Orbitrap-MS Technology

MA Danyang1,2, GAO Xueyan2, PENG Lingfeng2, WU Shaofeng1,2, WANG Qingtao3, HAO Zhihui1,2*   

  1. 1. Sanya Institute of China Agricultural University, Sanya 572025, China;
    2. Chinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
    3. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2023-04-12 Online:2023-12-23 Published:2023-12-26

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Abstract: To study the chemical composition of Areca catechu L. seed in deep processing, UHPLC-QE-Orbitrap-MS technology was used to analyze and identify the composition of Areca catechu L. seed. Gradient elution was performed on ACQUITY UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm) with 0.1% formic acid aqueous solution (A) to 0.1% formic acid acetonitrile solution (B) as mobile phases. The flow rate was 0.3 mL·min-1, the column temperature was 35 ℃, and the sample volume was 10 μL. An electric spray ion source (ESI) was used with a scanning range of 100-1 200 m/z and positive and negative ions were collected simultaneously, and the detection mode was Full MS-ddMS2. The original data were analyzed using Compound Discoverer 3.3 software, and the structures of the compounds were identified using the local database and mzCloud online database. Eighty-five compounds of Areca catechu L. seed in deep processing were detected, including 3 alkaloids, 24 flavonoids, 15 organic acids, 7 phenols, 7 terpenoids, 6 amino acids, 4 carbohydrates, 4 esters, 2 amides, 2 sphingolipids, 1 steroid, 1 phenylpropanoid, 1 coumarin and 8 other species. The UHPLC-QE-Orbitrap-MS detection technology quickly identified the compound of Areca catechu L. seed in deep processing, and the research results provided scientific basis for exploring the application prospects and developing high value-added products of Areca catechu L.

Key words: the seed of Areca catechu L., by-product, UHPLC-QE-Orbitrap-MS, composition

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